Longitudinal alterations in bone morphometry, mechanical integrity and composition in Type-2 diabetes in a Zucker diabetic fatty (ZDF) rat

Monahan, Genna E; Schiavi-Tritz, Jessica; Britton, Marissa; Vaughan, Ted J.

doi:10.1016/j.bone.2023.116672

Cited by 7 publications

(1 citation statement)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of such islands has been previously reported in ZDF rats 45 . Here, the higher prevalence of hypermineralised cartilage remnants in the sections of LepR −/− animals may be related to the impaired longitudinal growth of long bones in these animals 36 , a feature also observed in ZF (Zucker fatty) 60 and ZDF rats 61 . A recent study by Liddell et al 62 demonstrated continuous distal drift of the implants, with implants placed in the proximal tibial metaphysis of Wistar rats drifting to the diaphysis by 168 days of healing as a consequence of a normal endochondral ossification process at the epiphyseal growth plate.…”

Section: Discussionmentioning

confidence: 66%

Leptin receptor gene deficiency minimally affects osseointegration in rats

Jolic,

Ruscsák,

Emanuelsson

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Metabolic syndrome represents a cluster of conditions such as obesity, hyperglycaemia, dyslipidaemia, and hypertension that can lead to type 2 diabetes mellitus and/or cardiovascular disease. Here, we investigated the influence of obesity and hyperglycaemia on osseointegration using a novel, leptin receptor-deficient animal model, the Lund MetS rat. Machined titanium implants were installed in the tibias of animals with normal leptin receptor (LepR+/+) and those harbouring congenic leptin receptor deficiency (LepR−/−) and were left to heal for 28 days. Extensive evaluation of osseointegration was performed using removal torque measurements, X-ray micro-computed tomography, quantitative backscattered electron imaging, Raman spectroscopy, gene expression analysis, qualitative histology, and histomorphometry. Here, we found comparable osseointegration potential at 28 days following implant placement in LepR−/− and LepR+/+ rats. However, the low bone volume within the implant threads, higher bone-to-implant contact, and comparable biomechanical stability of the implants point towards changed bone formation and/or remodelling in LepR−/− rats. These findings are corroborated by differences in the carbonate-to-phosphate ratio of native bone measured using Raman spectroscopy. Observations of hypermineralised cartilage islands and increased mineralisation heterogeneity in native bone confirm the delayed skeletal development of LepR−/− rats. Gene expression analyses reveal comparable patterns between LepR−/− and LepR+/+ animals, suggesting that peri-implant bone has reached equilibrium in healing and/or remodelling between the animal groups.

show abstract