2015
DOI: 10.1097/mpa.0000000000000238
|View full text |Cite
|
Sign up to set email alerts
|

Longitudinal Bioluminescence Imaging of Primary Versus Abdominal Metastatic Tumor Growth in Orthotopic Pancreatic Tumor Models in NSG Mice

Abstract: Objectives The purpose of the present study was to develop and validate noninvasive bioluminescence imaging methods for differentially monitoring primary and abdominal metastatic tumor growth in mouse orthotopic models of pancreatic cancer. Methods A semiautomated maximum entropy segmentation method was implemented for the primary tumor region-of-interest, and a rule-based method for manually drawing a region-of-interest for the abdominal metastatic region was developed for monitoring tumor growth in orthoto… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

0
10
0

Year Published

2015
2015
2024
2024

Publication Types

Select...
6
1

Relationship

1
6

Authors

Journals

citations
Cited by 9 publications
(10 citation statements)
references
References 28 publications
0
10
0
Order By: Relevance
“…Additional cell lines expressing shMdm2 constructs (TRCN0000003376 and TRCN0000003377) were also examined to confirm the phenotypes were due to knockdown of Mdm2. Previously described vectors include shp53 (15,16), MCHERRY (17), and EGFP (17). Lentivirus was produced as previously described (16).…”
Section: Methodsmentioning
confidence: 99%
“…Additional cell lines expressing shMdm2 constructs (TRCN0000003376 and TRCN0000003377) were also examined to confirm the phenotypes were due to knockdown of Mdm2. Previously described vectors include shp53 (15,16), MCHERRY (17), and EGFP (17). Lentivirus was produced as previously described (16).…”
Section: Methodsmentioning
confidence: 99%
“…AsPC1, BxPC3, and Paca2 cells were obtained from the American Type Culture Collection (Rockville, MD), human pancreatic normal epithelial (HPNE) and Panc1 cells were a gift from Prof. Murray Korc (21). The normal human dermal fibroblasts (NHF544) were provided by Dr. Dan Spandau, and GFP-expressing neonatal dermal fibroblasts (GFP-HNDFs) were purchased from Angioproteomie (Boston, MA).…”
Section: Methodsmentioning
confidence: 99%
“…Orthotopic tumor mouse models are generally preferred over heterotopic subcutaneously-located pancreatic tumors because they offer tissue site-specific pathology, allow studies of metastasis, and are deemed more clinically relevant 33 , while the development of pancreatic tumors expressing luciferase/fluorescent proteins has facilitated the longitudinal monitoring of orthotopically located pancreatic tumors 34 . We next evaluated the efficacy of CK21 in an orthotropic xenograft model, using luciferase-transfected AsPC-1 implanted into the pancreas of nude mice and allowing the tumor to develop for 1-2 weeks before initiating CK21 treatment.…”
Section: Resultsmentioning
confidence: 99%
“…Human tumor organoids were obtained from patients with pancreatic ductal adenocarcinoma, confirmed to be tumor based on pathologic assessment, and developed into organoid culture according to established protocols 39 . Luciferase-transfected AsPC-1 tumors 34 , and mouse tumors from genetically KPC mice that spontaneously develop pancreatic cancer 47 have been previously described. CK21 was synthesized as described below.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation