Longitudinal Evaluation of Myocardial Fatty Acid and Glucose Metabolism in Fasted and Nonfasted Spontaneously Hypertensive Rats Using MicroPET/CT

Huber, J.; Hernandez, Andrew M.; Janabi, Mustafa; O’Neil, James P.; Brennan, Kathleen; Murphy, Stephanie T.; Seo, Youngho; Gullberg, Grant T.

doi:10.1177/1536012117724558

Cited by 6 publications

(8 citation statements)

References 41 publications

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“…These animals were obtained from an unrelated longitudinal study of perfusion, and fatty acid and glucose metabolism dysfunction using PET/CT. 16 The hearts of all SHRs exhibited LVH, confirmed by in vivo myocardial positron emission tomography studies. 16 In a second 2-year study from 2011 to 2012, it was again confirmed that the SHR model exhibits abnormal perfusion and metabolism.…”

Section: Imaging Protocolmentioning

confidence: 65%

“…16 The hearts of all SHRs exhibited LVH, confirmed by in vivo myocardial positron emission tomography studies. 16 In a second 2-year study from 2011 to 2012, it was again confirmed that the SHR model exhibits abnormal perfusion and metabolism. 17 Anesthetic overdose of isoflurane was used after which the heart was removed and the chambers flushed with saline solution delivered through the arterial valves.…”

Section: Imaging Protocolmentioning

confidence: 65%

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Quantitative analysis of hypertrophic myocardium using diffusion tensor magnetic resonance imaging

et al. 2016

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Systemic hypertension is a causative factor in left ventricular hypertrophy (LVH). This study is motivated by the potential to reverse or manage the dysfunction associated with structural remodeling of the myocardium in this pathology. Using diffusion tensor magnetic resonance imaging, we present an analysis of myocardial fiber and laminar sheet orientation in ex vivo hypertrophic (6 SHR) and normal (5 WKY) rat hearts using the covariance of the diffusion tensor. First, an atlas of normal cardiac microstructure was formed using the WKY b0 images. Then, the SHR and WKY b0 hearts were registered to the atlas. The acquired deformation fields were applied to the SHR and WKY heart tensor fields followed by the preservation of principal direction (PPD) reorientation strategy. A mean tensor field was then formed from the registered WKY tensor images. Calculating the covariance of the registered tensor images about this mean for each heart, the hypertrophic myocardium exhibited significantly increased myocardial fiber derangement (p ¼ 0.017) with a mean dispersion of 38.7 deg, and an increased dispersion of the laminar sheet normal (p ¼ 0.030) of 54.8 deg compared with 34.8 deg and 51.8 deg, respectively, in the normal hearts. Results demonstrate significantly altered myocardial fiber and laminar sheet structure in rats with hypertensive LVH.

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Section: Imaging Protocolmentioning

confidence: 65%

Section: Imaging Protocolmentioning

confidence: 65%

Quantitative analysis of hypertrophic myocardium using diffusion tensor magnetic resonance imaging

et al. 2016

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“…32 The discrepancy among these investigations may be attributable to the severity of HF, where in the early stages there is a normal (or slightly elevated) rate of fatty acid oxidation, with a downregulation in advanced stage of HF. 10 In all of our imaging studies 33,34 glucose metabolism was consistently elevated in the hypertensive SHRs compared to the normotensive WKY controls. When fed, the fatty acid metabolism in the SHRs was less than with the WKY controls.…”

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confidence: 75%

“…33 Whereas with fasting, there was an observed increase (though not significant) in fatty acid metabolism in the SHR model compared with WKY controls. 34 Metabolic changes appear to precede mechanical changes of LVH progression in the SHR model.…”

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confidence: 98%

PET imaging of glucose and fatty acid metabolism for NAFLD patients

Gullberg

Shrestha

Seo

2020

Journal of Nuclear Cardiology

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“… 14 Another group observed in older SHR (8–22 months of age), by longitudinal imaging with small animal positron emission tomography (PET)/computed tomography, increased glucose and fatty acid utilization at 8 months of age that was sustained up to 20 months. 15 , 16 Decreased systolic function and increased cardiac volume occurred only at 20 months.…”

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confidence: 99%

Progressive Cardiac Metabolic Defects Accompany Diastolic and Severe Systolic Dysfunction in Spontaneously Hypertensive Rat Hearts

Li,

Minczuk,

Huang

et al. 2023

JAHA

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Background Cardiac metabolic abnormalities are present in heart failure. Few studies have followed metabolic changes accompanying diastolic and systolic heart failure in the same model. We examined metabolic changes during the development of diastolic and severe systolic dysfunction in spontaneously hypertensive rats (SHR). Methods and Results We serially measured myocardial glucose uptake rates with dynamic 2‐[ 18 F] fluoro‐2‐deoxy‐ d ‐glucose positron emission tomography in vivo in 9‐, 12‐, and 18‐month‐old SHR and Wistar Kyoto rats. Cardiac magnetic resonance imaging determined systolic function (ejection fraction) and diastolic function (isovolumetric relaxation time) and left ventricular mass in the same rats. Cardiac metabolomics was performed at 12 and 18 months in separate rats. At 12 months, SHR hearts, compared with Wistar Kyoto hearts, demonstrated increased isovolumetric relaxation time and slightly reduced ejection fraction indicating diastolic and mild systolic dysfunction, respectively, and higher (versus 9‐month‐old SHR decreasing) 2‐[ 18 F] fluoro‐2‐deoxy‐ d ‐glucose uptake rates (Ki). At 18 months, only few SHR hearts maintained similar abnormalities as 12‐month‐old SHR, while most exhibited severe systolic dysfunction, worsening diastolic function, and markedly reduced 2‐[ 18 F] fluoro‐2‐deoxy‐ d ‐glucose uptake rates. Left ventricular mass normalized to body weight was elevated in SHR, more pronounced with severe systolic dysfunction. Cardiac metabolite changes differed between SHR hearts at 12 and 18 months, indicating progressive defects in fatty acid, glucose, branched chain amino acid, and ketone body metabolism. Conclusions Diastolic and severe systolic dysfunction in SHR are associated with decreasing cardiac glucose uptake, and progressive abnormalities in metabolite profiles. Whether and which metabolic changes trigger progressive heart failure needs to be established.

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Longitudinal Evaluation of Myocardial Fatty Acid and Glucose Metabolism in Fasted and Nonfasted Spontaneously Hypertensive Rats Using MicroPET/CT

Cited by 6 publications

References 41 publications

Quantitative analysis of hypertrophic myocardium using diffusion tensor magnetic resonance imaging

Quantitative analysis of hypertrophic myocardium using diffusion tensor magnetic resonance imaging

PET imaging of glucose and fatty acid metabolism for NAFLD patients

Progressive Cardiac Metabolic Defects Accompany Diastolic and Severe Systolic Dysfunction in Spontaneously Hypertensive Rat Hearts

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