Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model

Park, Hyunjoon; Yeo, Soyoung; Kang, Seok‐Won; Huh, Chul Sung

doi:10.3390/microorganisms9020370

Cited by 23 publications

(27 citation statements)

References 85 publications

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“…DSS could decrease the abundance of Bacteroidetes and increase the abundance of Firmicutes , while Dou et al [ 33 ] found that DSS could increase the abundance of Bacteroidetes . Park et al [ 34 ] concluded that the alteration of the composition of the gut microbiota by different concentrations of DSS was highly variable. This likely explains why this experimental data differed from previous studies.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Alleviates DSS-Induced Anxiety-Like Behaviors via the Microbial-Brain-Gut Axis

Zhang

Zhou

Chen

et al. 2022

Oxidative Medicine and Cellular Longevity

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The anxiety and depression caused by inflammatory bowel diseases (IBD) are known to greatly affect the mental health of patients. The mechanism of psychiatric disorders caused by IBD is not fully understood. Previous research has suggested that the gut microbiome plays a key role in IBD. Curcumin is a yellow polyphenol extracted from the rhizome of the ginger plant, which has been shown to have effects against both depression and anxiety. Research has indicated that curcumin affects the gut microbiome and exerts antianxiety and neuroprotective effects through the microbiota-gut-brain axis (MGB). However, whether curcumin can alleviate the psychiatric disorders caused by IBD and how curcumin affects the MGB axis through the gut microbiota have not been fully understood. Therefore, this study was aimed at determining the metabolic parameters and microbiological environment in the peripheral and central nervous system to determine the effects of curcumin against anxiety induced by dextran sulfate sodium salt (DSS) in mice. To elaborate on the link between the gut microbiota and how curcumin alleviates anxiety-like behaviors, we performed a fecal microbiota transplantation (FMT) experiment. The results suggested that curcumin can effectively relieve anxiety-like behaviors caused by DSS in mice. Further, curcumin treatment can alleviate disturbances in the gut microbiota and systemic disorders of lipid metabolism caused by DSS. Finally, through FMT, we verified that curcumin increased phosphatidylcholine in the prefrontal cortex of the mice and alleviated DSS-induced anxiety-like behaviors by modulating specific gut microbiota. We also revealed that Muribaculaceae may be a key part of the gut microbiota for curcumin to alleviate DSS-induced anxiety-like behaviors through the MGB axis.

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Section: Discussionmentioning

confidence: 99%

Curcumin Alleviates DSS-Induced Anxiety-Like Behaviors via the Microbial-Brain-Gut Axis

Zhang

Zhou

Chen

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Although Axl has been known to act as a negative regulator in the pathogenesis of IBD 18 , to the best of our knowledge, the effect of Axl on changes in the composition of the gut microbiota has not been investigated to date. Previous studies have demonstrated that IBD patients show a reduced abundance of the genera Bacteroides 65 , 66 , Alloprevotella 67 , Odoribacter 68 , Bifidobacterium , Eggerthella 69 , and Olsenella but an increased in Escherichia , Shigella , and Actinobacillus 70 . In addition, the species such as B. vulgatus , B. caccae , B. bifidum , and B. longum 68 are decreased in patients with IBD, whereas the species S. enterica 71 , E. coli 67 , A. segnis , H. parainfluenzae , and E. corrodens 68 are enhanced.…”

Section: Discussionmentioning

confidence: 96%

Axl alleviates DSS-induced colitis by preventing dysbiosis of gut microbiota

Yee

Choi

Seon

et al. 2023

Sci Rep

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Axl is a tyrosine kinase receptor, a negative regulator for innate immune responses and inflammatory bowel disease (IBD). The gut microbiota regulates intestinal immune homeostasis, but the role of Axl in the pathogenesis of IBD through the regulation of gut microbiota composition remains unresolved. In this study, mice with DSS-induced colitis showed increased Axl expression, which was almost entirely suppressed by depleting the gut microbiota with antibiotics. Axl−/− mice without DSS administration exhibited increased bacterial loads, especially the Proteobacteria abundant in patients with IBD, significantly consistent with DSS-induced colitis mice. Axl−/− mice also had an inflammatory intestinal microenvironment with reduced antimicrobial peptides and overexpression of inflammatory cytokines. The onset of DSS-induced colitis occurred faster with an abnormal expansion of Proteobacteria in Axl−/− mice than in WT mice. These findings suggest that a lack of Axl signaling exacerbates colitis by inducing aberrant compositions of the gut microbiota in conjunction with an inflammatory gut microenvironment. In conclusion, the data demonstrated that Axl signaling could ameliorate the pathogenesis of colitis by preventing dysbiosis of gut microbiota. Therefore, Axl may act as a potential novel biomarker for IBD and can be a potential candidate for the prophylactic or therapeutic target of diverse microbiota dysbiosis-related diseases.

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“…Another potential source of differential effects lies with the effects upon the gut microbiome, because different forms of BG might differently affect the gut microbiome composition [ 69 , 70 ]. While no differences in the healthy mice with BG (without DSS), Pachyman + DSS demonstrated the lowest beneficial Firmicute bacteria [ 40 , 71 ], and WGP + DSS produced the lowest Bacteroidetes [ 40 , 72 , 73 ], and Oat-BG + DSS did not alter the fecal microbiome. These subtle differences of bacterial abundance between BG + DSS vs. DSS alone were accompanied by a similar taxonomic diversity among these groups, which were supported by the ex vivo incubation of mouse feces with different BGs.…”

Section: Discussionmentioning

confidence: 99%

More Prominent Inflammatory Response to Pachyman than to Whole-Glucan Particle and Oat-β-Glucans in Dextran Sulfate-Induced Mucositis Mice and Mouse Injection through Proinflammatory Macrophages

Hiengrach

Visitchanakun

Finkelman

et al. 2022

IJMS

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(1→3)-β-D-glucans (BG) (the glucose polymers) are recognized as pathogen motifs, and different forms of BGs are reported to have various effects. Here, different BGs, including Pachyman (BG with very few (1→6)-linkages), whole-glucan particles (BG with many (1→6)-glycosidic bonds), and Oat-BG (BG with (1→4)-linkages), were tested. In comparison with dextran sulfate solution (DSS) alone in mice, DSS with each of these BGs did not alter the weight loss, stool consistency, colon injury (histology and cytokines), endotoxemia, serum BG, and fecal microbiome but Pachyman–DSS-treated mice demonstrated the highest serum cytokine elicitation (TNF-α and IL-6). Likewise, a tail vein injection of Pachyman together with intraperitoneal lipopolysaccharide (LPS) induced the highest levels of these cytokines at 3 h post-injection than LPS alone or LPS with other BGs. With bone marrow-derived macrophages, BG induced only TNF-α (most prominent with Pachyman), while LPS with BG additively increased several cytokines (TNF-α, IL-6, and IL-10); inflammatory genes (iNOS, IL-1β, Syk, and NF-κB); and cell energy alterations (extracellular flux analysis). In conclusion, Pachyman induced the highest LPS proinflammatory synergistic effect on macrophages, followed by WGP, possibly through Syk-associated interactions between the Dectin-1 and TLR-4 signal transduction pathways. Selection of the proper form of BGs for specific clinical conditions might be beneficial.

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Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model

Cited by 23 publications

References 85 publications

Curcumin Alleviates DSS-Induced Anxiety-Like Behaviors via the Microbial-Brain-Gut Axis

Curcumin Alleviates DSS-Induced Anxiety-Like Behaviors via the Microbial-Brain-Gut Axis

Axl alleviates DSS-induced colitis by preventing dysbiosis of gut microbiota

More Prominent Inflammatory Response to Pachyman than to Whole-Glucan Particle and Oat-β-Glucans in Dextran Sulfate-Induced Mucositis Mice and Mouse Injection through Proinflammatory Macrophages

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