Longitudinal study on the effects of intramammary infection with non-aureus staphylococci on udder health and milk production in dairy heifers

Valckenier, Dimitri; Piepers, Sofie; Schukken, Y.H.; Visscher, Alex De; Boyen, Filip; Haesebrouck, Freddy; Vliegher, Sarne De

doi:10.3168/jds.2020-18685

Cited by 27 publications

(22 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study relate to the findings of Piessens et al ( 2011 ) that identified S. chromogenes as the most prominent NAS and S. epidermidis, S haemolyticus and S. simulans as frequently isolated by other studies (Dalen et al 2017 ; Vanderhaeghen et al 2015 ). The three species of NAS isolates identified in this study agreed with the findings from other studies conducted in countries such as Canada, Belgium (4 studies), Finland, the Netherlands, the United States, Poland and Argentina (Condas et al 2017 ; De Visscher et al 2016 , 2017 ; Jenkins et al 2019 ; Valckenier et al 2021 ). The prevalence of each species of NAS in this study (Appendix 1, Table S3) differed from that found in a study performed in Switzerland, which found that the most prevalent NAS species were S. xylosus (35%), S. vitulinus (10%) and S. chromogenes (7%), whereas S. chromogenes (5%), S. xylosus (5%) and S. haemolyticus (4%) isolates were least prevalent (Jenkins et al 2019 ).…”

Section: Prevalence and Species Identification Of Non-aureus Staphylo...supporting

confidence: 90%

Species identification and cow risks of non-aureus staphylococci from South African dairy herds

Petzer

Labuschagne

Phophi

et al. 2022

Onderstepoort j. vet. res.

View full text Add to dashboard Cite

Detailed information on specific species of non-aureus staphylococci (NAS) has become a necessity for effective udder health control programs in South Africa. The main objective of this preliminary study was to identify the different NAS species and strains present in dairy herds in South Africa using a cost-effective method. A further objective was to investigate the effects of cow risk factors and farming systems on the NAS isolates identified. A total of 214 NAS, isolated from milk collected from 17 South African dairy herds, were identified using three diagnostic tests (API Staph test, MALDI-TOF and 16s rRNA). There was a good observed agreement between the MALDI-TOF and 16S rRNA sequencing (92.2%) and a poor observed agreement between the MALDI-TOF and API Staph (25.7%). The genetic relatedness within species was investigated in 128 of these isolates using random polymorphic amplified deoxyribonucleic acid (DNA) (RAPD), verified by multilocus sequence typing (MLST), and phylogenetic analysis and cow risk factors were investigated on species level. The main NAS species isolated were Staphylococcus chromogenes (75.2%), Staphylococcus epidermidis (9.4%) and Staphylococcus haemolyticus (8.9%). The RAPD test identified 34 Staphylococcus chromogenes, 13 Staphylococcus epidermidis and nine Staphylococcus haemolyticus strains, indicating genetic diversity amongst strains and herds. The presence of NAS intramammary infections was found to be significantly related to the farming systems, composite cow milk somatic cell count (SCC), parity and days in milk (DIM). Significantly more NAS were isolated from primiparous and from older cows. This knowledge could assist with the management of NAS on dairy farms.

show abstract

Section: Prevalence and Species Identification Of Non-aureus Staphylo...supporting

confidence: 90%

Species identification and cow risks of non-aureus staphylococci from South African dairy herds

Petzer

Labuschagne

Phophi

et al. 2022

Onderstepoort j. vet. res.

View full text Add to dashboard Cite

show abstract

“…The role of NAS for bovine udder health is under scrutiny, with recent work discussing a wide diversity between species and even strains in epidemiology, ecology, virulence, and host-interaction [ 17 – 21 ]. Previous studies reported NAS as a relevant cause of bovine mastitis [ 17 , 22 , 23 ], while others reported only a slight increase of the milk somatic cell count (SCC) as an indicator of mammary gland inflammation [ 24 , 25 ] and no impact on milk yield, despite the elevated SCC [ 26 ]. A protective effect of NAS intramammary infections or NAS teat apex colonization against intramammary infections caused by major pathogens has also been reported [ 27 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

Silva

Souza

Piepers

et al. 2021

Vet Res

View full text Add to dashboard Cite

Communications via quorum sensing (QS) between non-aureus staphylococci (NAS) and Staphylococcus (S.) aureus in the bovine mammary gland remains largely unexplored. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices were able to regulate the QS of S. aureus, and if so, how in vitro growth inhibition of S. aureus by NAS, or NAS metabolites, or NAS cells themselves play a role in this process. In co-culture with S. aureus we observed that these 3 NAS species in general downregulated the expression of rnaIII, the effector molecule of the QS system, but this effect was more pronounced in S. chromogenes and S. simulans isolates than in S. epidermidis isolates. In vitro growth inhibition of S. aureus by NAS resulted in a small underestimation of the downregulating effect of NAS on rnaIII expression of S. aureus. Additionally, the culture supernatant of these NAS isolates and supernatant treated with proteinase K expressed greater regulatory activity over S. aureus virulence genes rnaIII, hla, and spa than washed NAS cells suspended in sterile water. These microbial interactions may influence S. aureus virulence and pathogenesis within the host. Isolation and identification of NAS metabolites affecting the QS system of S. aureus might help to develop alternative strategies for treatment and control of S. aureus mastitis.

show abstract

“…Even though differences between bovine NAS species and strains have been extensively studied, several of their characteristics, including the interaction with the host, remain undefined. Several NAS typically cause a moderate increase in milk somatic cell count (SCC) [14] yet do not negatively impact milk yield [15][16][17], and can inhibit major mastitis pathogen growth in vitro [18]. A protective effect against clinical mastitis has been suggested as well [16,19,20].…”

Section: Introductionmentioning

confidence: 99%

Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Beuckelaere

Visscher

Souza

et al. 2021

Vet Res

View full text Add to dashboard Cite

Although extensive research has been performed on bovine non-aureus staphylococci (NAS), several aspects such as bacteria-host interaction remain largely unstudied. Moreover, only a few mastitis pathogen challenge studies in cows have been conducted in the dry period, an important period that allows intramammary infection (IMI) to cure and new IMI to occur. We challenged 16 quarters of 4 Holstein Friesian cows at dry off with 100; 100 000 or 10 000 000 CFU of the udder-adapted S. chromogenes IM strain. Four quarters from one cow served as negative controls. Internally sealed quarters remained untouched, whereas non-sealed quarters were sampled 3 times during the dry period. After parturition, colostrum and daily milk samples were taken during the first week of lactation of all quarters. In total, 8 quarters appeared to be colonized, since S. chromogenes IM was recovered at least once during the experiment, as substantiated using Multilocus Sequence Typing. S. chromogenes IM shedding was highest in dry quarters inoculated with 10 000 000 CFU. Colonized quarters had the highest quarter somatic cell count (qSCC) in early lactation. Inoculated quarters (both colonized and non-colonized) had lower IL-6 and IL-10 concentrations in the dry period, whilst IFN-γ levels tended to be higher in colonized quarters compared to non-inoculated quarters. Also, IgG2 levels were higher in inoculated compared to non-inoculated quarters and the IgG2/IgG1 ratio was on average above 1. To conclude, we showed that dry quarters can be colonized with S. chromogenes IM, resulting in a shift towards a Th1 response in late gestation and early lactation characterised by an increased IgG2 concentration. However, further research is needed to confirm our findings.

show abstract

Longitudinal study on the effects of intramammary infection with non-aureus staphylococci on udder health and milk production in dairy heifers

Cited by 27 publications

References 72 publications

Species identification and cow risks of non-aureus staphylococci from South African dairy herds

Species identification and cow risks of non-aureus staphylococci from South African dairy herds

Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro

Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Contact Info

Product

Resources

About