Longterm Exercise-Derived Exosomal miR-342-5p

Hou, Zuoxu; Qin, Xinghua; Hu, Yuanyuan; Xing, Zhen; Li, Guohua; Wu, Jie; Li, Jia; Sha, Jianding; Chen, Jiangwei; Xia, Jielai; Wang, Lifeng; Gao, Feng

doi:10.1161/circresaha.118.314635

Cited by 236 publications

(142 citation statements)

References 45 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…Among those differentially expressed exosomal miRNAs, a few studies on their functions and mechanisms in diseases had been performed. For instance, exosomal miR‐342‐5p was confirmed to protect the heart against the myocardial ischemia/reperfusion injury 22 . CCAT1/miR‐185‐3p/MLCK signaling pathway promotes the inflammatory bowel disease malignancy by destroying the intestinal barrier 23 .…”

Section: Discussionmentioning

confidence: 99%

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

et al. 2020

View full text Add to dashboard Cite

Microscopic polyangiitis (MPA) is a systemic autoimmune disease that primarily affects the small and medium blood vessels. Endothelial injury is one of the pathological hallmarks of MPA. However, the pathogenesis for this has not yet been fully elucidated. Exosomal microRNAs (miRNAs) have recently emerged as a new molecular pattern involved in the endothelial injury in other diseases. Hence, we speculated that MPA plasma-derived exosomes (MPA-exo) could induce the endothelial injury, which was likely to be aroused by the dysregulated exosomal miRNAs in MPA. In the present study, plasma-derived exosomes were isolated and identified. MPA-exo could be internalized by human renal glomerular endothelial cells (HRGECs) in vitro and induced HRGECs injury. Subsequently, a series of differentially expressed miRNAs in MPAexo were identified by high-throughput sequencing analysis. Further bioinformatics analysis for the target genes of these differentially expressed miRNAs showed a potential mechanism for their possible role in MPA endothelial injury. Notably, we revealed a considerable correlation between miR-185-3p, miR-125a-3p, and clinical parameters. In conclusion, the current study revealed that differentially expressed miRNAs in MPA-exo are associated with the endothelial injury. Our results suggested that these miRNAs and their target genes might be involved in the inflammation process of MPA. K E Y W O R D Shigh-throughput sequencing, microRNA profile, plasma exosomes 6216 | WANG et Al.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Serum‐free conditioned medium was collected after treatment of ECs. Exosomes were also isolated with the ExoQuick Plasma prep and the Exosomes Precipitation Kit according to the manufacturer's instructions (System Biosciences) …”

Section: Methodsmentioning

confidence: 99%

Exosome derived from CD137‐modified endothelial cells regulates the Th17 responses in atherosclerosis

Geng

Zang

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

| 4659 wileyonlinelibrary.com/journal/jcmm 1 | INTRODUC TI ON T helpers 17 (Th17) cells have been identified as a new subset of lymphocytes which are shown to promote atherosclerosis (AS), and their associated cytokines in the pathogenesis of AS appear to be contradictory at first glance. 1-3 Recently, growing attention has been paid to the relative contribution of the local vs. peripheral inflammation to the procession of AS. Here, we focused on the communication between peripheral inflammation and vascular inflammation of Th17 cell differentiation during AS.Exosomes are a subset of extracellular vesicles (EVs) released by almost all types of cells, and they play a significant role in cellular proliferation, apoptosis, stress response and differentiation in the initial stage and development of AS. 4,5 In the present study, we investigated exosome-mediated cell-to-cell communication, which induced Th17 cell differentiation and promoted progression of the atherosclerotic plaque. AbstractThe role of exosomes derived from endothelial cells (ECs) in the progression of atherosclerosis (AS) and inflammation remains largely unexplored. We aimed to investigate whether exosome derived from CD137-modified ECs (CD137-Exo) played a major role in AS and to elucidate the potential mechanism underlying the inflammatory effect. Exosomes derived from mouse brain microvascular ECs treated with agonist anti-CD137 antibody were used to explore the effect of CD137 signalling in AS and inflammation in vitro and vivo. CD137-Exo efficiently induced the progression of AS in ApoE −/− mice. CD137-Exo increased the proportion of Th17 cells both in vitro and vivo. The IL-6 contained in CD137-Exo which is regulated by Akt and NF-КB pathway was verified to activate Th17 cell differentiation. IL-17 increased apoptosis, inhibited cell viability and improved lactate dehydrogenase (LDH) release in ECs subjected to inflammation induced by lipopolysaccharide (LPS). The expression of soluble intercellular adhesion molecule1 (sICAM-1), monocyte chemoattractant protein-1 (MCP-1) and E-selectin in the supernatants of ECs after IL-17 treatment was dramatically increased. CD137-Exo promoted the progression of AS and Th17 cell differentiation via NF-КB pathway mediated IL-6 expression. This finding provided a potential method to prevent local and peripheral inflammation in AS. K E Y W O R D S atherosclerosis, endothelial cells; Th17 cells, exosomes

show abstract

“…It has been well established that in vitro H/R model can follow in vivo myocardial I/R injury [26,27]. In the current study, we performed the hypoxia/reoxygenation model, one that has been widely used to investigate the mechanisms of myocardial I/R injury [26,27].…”

Section: Introductionmentioning

confidence: 99%

Critical roles of microRNA-141-3p and CHD8 in hypoxia/reoxygenation-induced cardiomyocyte apoptosis

et al. 2020

View full text Add to dashboard Cite

Background: Cardiovascular diseases are currently the leading cause of death in humans. The high mortality of cardiac diseases is associated with myocardial ischemia and reperfusion (I/R). Recent studies have reported that micro-RNAs (miRNAs) play important roles in cell apoptosis. However, it is not known yet whether miR-141-3p contributes to the regulation of cardiomyocyte apoptosis. It has been well established that in vitro hypoxia/reoxygenation (H/R) model can follow in vivo myocardial I/R injury. This study aimed to investigate the effects of miR-141-3p and CHD8 on cardiomyocyte apoptosis following H/R. Results:We found that H/R remarkably reduces the expression of miR-141-3p but enhances CHD8 expression both in mRNA and protein in H9c2 cardiomyocytes. We also found either overexpression of miR-141-3p by transfection of miR-141-3p mimics or inhibition of CHD8 by transfection of small interfering RNA (siRNA) significantly decrease cardiomyocyte apoptosis induced by H/R. Moreover, miR-141-3p interacts with CHD8. Furthermore, miR-141-3p and CHD8 reduce the expression of p21. Conclusion:MiR-141-3p and CHD8 play critical roles in cardiomyocyte apoptosis induced by H/R. These studies suggest that miR-141-3p and CHD8 mediated cardiomyocyte apoptosis may offer a novel therapeutic strategy against myocardial I/R injury-induced cardiovascular diseases.

show abstract

Longterm Exercise-Derived Exosomal miR-342-5p

Cited by 236 publications

References 45 publications

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

Exosome derived from CD137‐modified endothelial cells regulates the Th17 responses in atherosclerosis

Critical roles of microRNA-141-3p and CHD8 in hypoxia/reoxygenation-induced cardiomyocyte apoptosis

Contact Info

Product

Resources

About