Looking at mechanobiology through an evolutionary lens

Radin, Ivan; Haswell, Elizabeth S.

doi:10.1016/j.pbi.2021.102112

Cited by 4 publications

(1 citation statement)

References 62 publications

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“…Alternatively, structural stabilization of chloroplasts should be considered a major property of the plastidic PGN layers. Another hypothesis in this regard has been suggested recently 47 that the PGN layer, in combination with the plastoskeleton, contributes to the integrity and mechanical stability of chloroplasts.…”

Section: Because Of the Normal Chloroplast Division In The Cells Of A...mentioning

confidence: 97%

The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

Tran

Keskin

Winkler

et al. 2023

Preprint

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Plastids in plants are assumed to have evolved from cyanobacteria as they have maintained several bacterial features. Recently, peptidoglycans, as bacterial cell wall components, have been shown in the envelopes of moss chloroplasts. Phylogenomic comparisons of bacterial and plant genomes have raised the question of whether such structures are also part of chloroplasts in angiosperms. To address this question, we visualized canonical amino acids of peptidoglycan around chloroplasts of Arabidopsis and Nicotiana via click chemistry and fluorescence microscopy. Additional detection by different peptidoglycan-binding proteins from bacteria and animals supported this observation. Further Arabidopsis experiments with D-cycloserine and AtMurE knock-out lines, both affecting putative peptidoglycan biosynthesis, revealed a central role of this pathway in plastid genesis and division. Taken together, these results indicate that peptidoglycans are integral parts of plastids in the whole plant lineage. Elucidating their biosynthesis and further roles in the function of these organelles is yet to be achieved.

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Section: Because Of the Normal Chloroplast Division In The Cells Of A...mentioning

confidence: 97%

The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

Tran

Keskin

Winkler

et al. 2023

Preprint

View full text Add to dashboard Cite

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Rapid Stiffness Mapping in Soft Biologic Tissues With Micrometer Resolution Using Optical Multifrequency Time‐Harmonic Elastography

Jordan,

Jaitner,

Meyer

et al. 2024

Advanced Science

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Rapid mapping of the mechanical properties of soft biological tissues from light microscopy to macroscopic imaging can transform fundamental biophysical research by providing clinical biomarkers to complement in vivo elastography. This work introduces superfast optical multifrequency time‐harmonic elastography (OMTHE) to remotely encode surface and subsurface shear wave fields for generating maps of tissue stiffness with unprecedented detail resolution. OMTHE rigorously exploits the space‐time propagation characteristics of multifrequency time‐harmonic waves to address current limitations of biomechanical imaging and elastography. Key solutions are presented for stimulation, wave decoding, and stiffness reconstruction of shear waves at multiple harmonic frequencies, all tuned to provide consistent stiffness values across resolutions from microns to millimeters. OMTHE's versatility is demonstrated by simulations, phantoms, Bacillus subtilis biofilms, zebrafish embryos and adult zebrafish, reflecting the diversity of biological systems from a mechanics perspective. By zooming in on stiffness details from coarse to finer scales, OMTHE has the potential to advance mechanobiology and offers a way to perform biomechanics‐based tissue histology that consistently matches in vivo time‐harmonic elastography in patients.

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Exploring Force-Driven Stochastic Folding Dynamics in Mechano-Responsive Proteins and Implications in Phenotypic Variation

Rakshit,

Saha,

Vashisht

et al. 2024

Preprint

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Single-point mutations are pivotal in molecular zoology, shaping functions and influencing genetic diversity and evolution. Here we study three such genetic variants of a mechano-responsive gating-spring protein, cadherin-23, that uphold the structural integrity of the protein, but showcase distinct genotypes and phenotypes. All-atom simulations indicated marginal deviations in the transient intra-domain interactions among the variants leading to variations in the anti-cross correlated motions among constituent β-strands. In nature, the variants experience declining functions with aging at different rates. We expose these variants to constant and oscillatory forces using magnetic tweezer, and measure variations in stochastic folding dynamics. All variants exhibit multiple microstates under force. However, the protein variant with higher number of intra-domain contacts exhibits transitions among the heterogeneous microstates for larger extent of forces and persisted longer. Conversely, the protein variant with weaker inter-strand correlations exhibits greater unfolding cooperativity and faster intrinsic folding, although its folding-energy landscape is more susceptible to distortion under tension. Our study thus deciphers the molecular mechanisms underlying the variations in force-adaptations and propose a mechanical relation between genotype and phenotype.

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Looking at mechanobiology through an evolutionary lens

Cited by 4 publications

References 62 publications

The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

The Chloroplast Envelope of Angiosperms Contains a Peptidoglycan Layer

Rapid Stiffness Mapping in Soft Biologic Tissues With Micrometer Resolution Using Optical Multifrequency Time‐Harmonic Elastography

Exploring Force-Driven Stochastic Folding Dynamics in Mechano-Responsive Proteins and Implications in Phenotypic Variation

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