Loop-mediated isothermal amplification: Beyond microbial identification

Kundapur, Rajesh R.; Nema, Vijay

doi:10.1080/23312025.2015.1137110

Cited by 22 publications

(13 citation statements)

References 26 publications

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“…Applications of LAMP also extend to many other fields beyond in vitro diagnostics, as summarized in several recent reviews, such as species authentication and microbiological quality/safety assessment in meats (Kumar et al , 2017 ), and testing for genetically modified organisms (GMOs), allergens, pesticides, and drug resistance (Kundapur and Nema, 2016 ). A quick PubMed search using the term “loop-mediated isothermal amplification” returned >2100 articles, highlighting the great interest in LAMP within the scientific community.…”

Section: Lamp In a Nutshellmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed.

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Section: Lamp In a Nutshellmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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“…This assay is currently used as standalone diagnostic test for Clostridium difficile infection [ 34 ]. In addition to above developments, LAMP assay can be extensively applied in the field of molecular diagnosis of cancer, identification of genetically modified organisms, detection of food adulteration, eutrophication, food allergens, pesticides, identification of medicinal plants, drug resistance, and DNA methylation studies [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases

et al. 2016

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India being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be a development of rapid, reliable, robust diagnostic technique, which can work with high degree of sensitivity and specificity. Loop mediated isothermal amplification assay is a rapid gene amplification technique that amplifies nucleic acid under an isothermal condition with a set of designed primers spanning eight distinct sequences of the target. This assay can be used as an emerging powerful, innovative gene amplification diagnostic tool against various pathogens of livestock diseases. This review is to highlight the basic concept and methodology of this assay in livestock disease.

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“…As a result, LAMP has been used in a wide range of applications to overcome the limitations of PCR (Becherer et al 2020;Kundapur and Nema 2016;Martzy et al 2019;Mori et al 2013;Tasrip et al 2019). Among those applications, LAMP can be combined with other systems to allow better detection of the amplified products.…”

Section: Introductionmentioning

confidence: 99%

Combination of loop-mediated isothermal amplification and AuNP-oligoprobe colourimetric assay for pork authentication in processed-meat products

Thangsunan¹,

Temisak²,

Morris³

et al. 2020

Preprint

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Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well as those who are allergic to pork meat and its derivatives. Accurate pork authentication is of great importance to assist this demographic group of people in making decision on their product purchase. The aim of this study was to develop a new analytical method for pork authentication in processed-meat products based on a combination of loop-mediated isothermal amplification (LAMP) and AuNP-nanoprobe colourimetric assay. The LAMP conditions were first optimised to obtain the highest yield of amplified DNA products within the shortest time. Oligoprobe-functionalised AuNPs were then hybridised with LAMP-DNA amplicons, and subsequently challenged with MgSO4 at a high concentration to induce AuNP aggregation. In the presence of pork DNA, the colloidal AuNPs-probe remained unchanged in its red colour, which indicates the dispersion of AuNPs. In contrast, in the absence of pork DNA, the colour was changed to colourless as a result from the aggregation of AuNPs. The LAMP-AuNP nanoprobe assay offers a high sensitivity with a limit of detection as low as 100 pg of pork DNA. The assay is highly specific to pork content without crossreactivity with the other meat species tested. The assay developed herein can become a simple, inexpensive, precise, and rapid analytical tool for small laboratories or the general public interested in halal food authentication. Keywords: pork authentication; pork adulteration; halal food authentication; loop-mediated isothermal amplification; AuNP-nanoprobe colourimetric assay

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Loop-mediated isothermal amplification: Beyond microbial identification

Cited by 22 publications

References 26 publications

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases

Combination of loop-mediated isothermal amplification and AuNP-oligoprobe colourimetric assay for pork authentication in processed-meat products

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