Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Abstract

Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed.

A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene

“…Results indicate that this method is as specific as standard PCR methods and is extremely sensitive. The detection limit is 7.3 × 10 1 CFU/mL, which is more sensitive than that in many previous reports ( Yang et al., 2018 ) and is 1,000-fold greater than that in the national standard PCR method. The comparison between results of the clinical test used the visualized LAMP method, and those using the national standard PCR method showed that both methods caused missed detections.…”

“…Existing methods of LAMP observation mainly include turbidity analysis, gel electrophoresis, the addition of fluorescent dyes, and colorimetry. Furthermore, some methods also require special expensive monitoring equipment or the opening of tube lids after the reaction ( Yang et al., 2018 ). This can easily cause aerosol contamination and is not suitable for clinical use.…”

<p>Establishment and Evaluation of a Novel Method Based on Loop-Mediated Isothermal Amplification for the Rapid Diagnosis of Thalassemia Genes</p>

“…In recent years, the LAMP assay has been widely used in food safety, pathogen and microbiological testing, clinical diagnosis, and so on. [6][7][8][9] LAMP assays are also an effective tool for screening genetic mutations. 10 A previous study detected the -SEA (Southeast Asian) deletion, and IVSII-I G>A mutation indicating thalassemia using LAMP assays.…”

Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis

“…Various commercial products combining isothermal DNA amplification assays with portable platforms have become available in recent years. While most of them detect causative agents in clinical specimens, some platforms also employ ready-to-use test kits for applications in food and environmental analysis [ 24 ]. This includes the Loopamp Realtime Turbidimeter, an instrument for measuring the turbidity generated by the LAMP reaction in real time in combination with reagent kits to detect pathogens in foods and water (e.g., Salmonella , Listeria , Legionella , and Cryptosporidium ).…”

“…Among the four steps covered in this section, this is where most of the work has been done in recent years. Moreover, many isothermal DNA amplification methods targeting important pathogens have already been diligently studied or evaluated, such as LAMP assays to detect Salmonella in food and feed by the Food and Drug Administration of the United States [ 24 ]. Although the abovementioned MIQE guidelines have been defined primarily for qPCR experiments, it will be of utmost importance to extend them to isothermal DNA amplification assays to establish certain quality standards in the future.…”