Loop-mediated  isothermal  amplification  (LAMP) technique  in  microdevice  format   (Review)

Zubik, A. N.; Rudnitskaya, G. E.; Evstrapov, A. A.

doi:10.18358/np-31-1-i343

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…As a rule, during the process of DNA analysis, amplification of certain DNA sectors occurs, which are further investigated [9]. There are also methods that allow amplifying DNA at a constant temperature, without any cyclic change in temperature, peculiar for PCR, for example, such methods include loopmediated isothermal amplification (LAMP) [8,17]. Other similar methods are cross-priming amplification (CPA), recombinase polymerase amplification (RPA) and SEA amplification (Denaturation Bubble-mediated Strand Exchange Amplification) [18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Review of new technologies used for meat identification

Kornienko

Fomina²,

Minaev

2022

Teor. prakt. pererab. mâsa (Print)

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The present article represents an analysis of trends in development of test-systems for identification of meat. These test systems are commonly used in food production and research laboratories. The relevance of development of methods for identification of meat kinds is related not only to the food restrictions, which are practiced in some religions and related to consumption of certain types of meat, but also with the hygienic aspects of food production. Also, this research is inspired also by the acute issue of food products adulteration and the replacement of one type of meat with another one. The article considers the trends in the develop ment of microanalysis method that use immunochromatic research, i. e. methods based on molecular biology. Also this article considers the devices that do not use chromatographic methods of analysis. Examples of the development of test systems based on various methods of analysis for the identification of meat are given below. Attention is focused on the prospects of combining these methods, including colorimetric methods for identification of meat. It is also specified that the emergence of new dyes and new enzyme systems, suitable for use in enzyme-immunoassay, can enhance the sensitivity of these test systems. It is also noted that the development of technologies associated with sorbents can contribute to a better separation of the test substrates and this way to increase the sensitivity of the test in case of small amounts of test substrate. It is also noted that the use of various types of iso thermal amplification can reduce the analysis time necessary for meat identification. Various schemes of devices for microanalysis are given; their advantages and disadvantages are listed. An example of proteomes application for meat identification is given. It is shown that this method can also be applied in the heat treatment of meat. The prospects for the development of such devices are analyzed. It is concluded that the development of systems for microanalysis in the form of quick tests is quite relevant and promis ing. It is indicated that theoretically in the future such analytical systems, due to the use of microfluidic technologies, will be able to combine several methods. The authors proposed to use machine-aided cognition methods to analyze data obtained from similar test systems in order to increase their sensitivity.

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Section: Introductionmentioning

confidence: 99%

Review of new technologies used for meat identification

Kornienko

Fomina²,

Minaev

2022

Teor. prakt. pererab. mâsa (Print)

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Experience in developing reagent kits for identifying pathogens of tick-borne infections using isothermal amplification

Komarovsky,

Gorbenko,

Vasilyeva

et al. 2024

Lab. Service

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Directions of the laboratory diagnostics improving of the socially significant hemocontact viral infections in the military medical organizations

Orlova¹,

Bulan'kov²,

Sechin³

2022

Russian Military Medical Academy Reports

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In this study we have shown the relevance of the nucleic acid testing for the diagnosis of the human immunodeficiency virus infection and hemocontact viral hepatitis in the military medical organizations as well as the experience of majority of the foreign countries that actively apply modern molecular methodologies for the diagnosis of infectious diseases. The possibility of using the rapid tests, molecular biology techniques and an alternative carrier of the biological material the dry drop method, the potential of this method of biological material preparation in infectious diseases diagnostics in case of laboratory remoteness and for follow-up observation of patients with socially significant hemocontact viral infections is shown. New algorithm of human immunodeficiency virus infection laboratory testing using polymerase chain reaction as an expert test followed by methods of determination of recency of acquisition of human immunodeficiency virus antibodies especially in questionable laboratory result cases are proposed. Promising molecular genetic methods for implementation in laboratories in military medical organizations such as nester-polymerase chain reaction, loop-mediated isothermal amplification, digital polymerase chain reaction, mass spectrometry of polymerase chain reaction amplicons, Deoxyribonucleic acid sequence are observed. We have defined the main approaches to improve laboratory diagnostics of social significant hemocontact viral infections in military medical organizations: optimization of logistics of pre-analytical step with expansion of validated biomaterial samples storage and delivery methods (including dry drop); reduction in the duration of analytical phase through increase in the clinical use of polymerase chain reaction testing (including isothermic variant); improving the sensitivity and accuracy of serological and molecular diagnostics; development of validation methodology and laboratory tests results interpretation While ensuring military medical expertise. The necessity of specialists training in new diagnostics techniques and result interpretation, improving regulatory framework on examination and registration of human immunodeficiency virus positive military officers, determination of the legal status of cases with unfinished human immunodeficiency virus diagnostic process in order to ensure timely preventive and treatment care events and to objectify statistical recording of this category.

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Loop-mediated isothermal amplification (LAMP) technique in microdevice format (Review)

Cited by 5 publications

References 2 publications

Review of new technologies used for meat identification

Review of new technologies used for meat identification

Experience in developing reagent kits for identifying pathogens of tick-borne infections using isothermal amplification

Directions of the laboratory diagnostics improving of the socially significant hemocontact viral infections in the military medical organizations

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