Loquat (Eriobotrya Lindl.)

Badenes, María Luisa; Lin, Shunquan; Yang, Xianghui; Liu, Chengming; Huang, Xuming

doi:10.1007/978-0-387-77491-6_25

Cited by 14 publications

(8 citation statements)

References 25 publications

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“…Although the crop improvement of loquat is being carried out both by breeding programs and selection accessions from germplasm resources, the varieties grown mostly come from selections (Badenes et al, 2009). One reason for the slow breeding program in loquat is the existing of its long juvenile phase that last for several years.…”

Section: Discussionmentioning

confidence: 99%

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

et al. 2017

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As a master regulator involved in flower development, LEAFY-like gene has been demonstrated to play a key role in the flowering process regulation of angiosperms. Expression analysis of EjLFY-1, a LEAFY (LFY) homolog of loquat (Eriobotrya japonica Lindl.), indicated its participation in the regulation of flowering in loquat. To verify its function and potential value in the genetic engineering to shorten the juvenile phase, ectopic expression of EjLFY-1 in strawberry (Fragaria × ananassa) was achieved using Agrobacterium-mediated gene transfer of a plant expression vector with the loquat EjLFY-1 gene driven by the CaMV 35S promoter. Totally 59 plantlets were verified to be the transformants. The presence, expression and integration of EjLFY-1 in the transformants were assessed by PCR, quantitative real-time PCR and Southern blot, respectively. Constitutive expression of EjLFY-1 in strawberry accelerated the flowering process in strawberry with the shorten necessary period for flowering induction, development of flower and fruit set. While vegetative growth habits of the transformants in the first cropping season were consistent with the WT ones. Meanwhile, both the flowers and fruits of the transformants were also as same as those of the WT ones. Furthermore, the early-flowering habit was maintained in their asexual progeny, the runner plants. While with continuous asexual propagation, the clones showed a more strengthen early-flowering phenotype, such as the reduced vegetative growth and the abnormal floral organs in individual plantlets. These results demonstrated the function of this gene and at the same time provided us new insights into the utilization potential of such genes in the genetic engineering of perennial fruits.

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Section: Discussionmentioning

confidence: 99%

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

et al. 2017

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“…Loquat sets fruit in fall or early winter and as such provides appealing, nutritious and succulent pome fruits in the off-season during spring to early summer [1]; thus, it is a promising fruit tree for cultivation worldwide in the next decades [3]. In addition to classical breeding, molecular studies aim to develop or identify molecular markers/genes associated with specific traits that contributes to improving this fruit.…”

Section: Discussionmentioning

confidence: 99%

“…Loquat is primarily cultivated in Southeast Asia and Mediterranean regions [1] and differs from temperate-zone relatives, such as apple, pear and peach, which normally flower in the spring, by flowering in fall or early winter [2]. Loquat fruits mature during the slack season of the fresh fruit market in late spring or early summer, and the nutritious and succulent fruits are attracting an increasing number of consumers worldwide [3]. In addition, loquat is also used in traditional Chinese medicine due to an abundance of therapeutic compounds and secondary metabolites in its leaves and other tissues, as well as the presence of anti-cancer compounds [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Yuan

Zhang

et al. 2019

Plant Methods

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BackgroundLoquat (Eriobotrya japonica) is a subtropical tree bearing fruit that ripens during late spring and early summer, which is the off-season for fruit production. The specific flowering habit of loquat, which starts in fall and ends in winter, has attracted an increasing number of researchers who believe that it may represent an ideal model for studying flowering shift adaptations to climate change in Rosaceae. These studies require an understanding of gene expression patterns within the fruit and other tissues of this plant. Although ACTINs (ACTs) have previously been used as reference genes (RGs) for gene expression studies in loquats, a comprehensive analysis of whether these RGs are optimal for normalizing RT-qPCR data has not been performed.ResultsIn this study, 11 candidate RGs (RIBOSOMAL-LIKE PROTEIN4 (RPL4), RIBOSOMAL-LIKE PROTEIN18 (RPL18), Histone H3.3 (HIS3), Alpha-tubulin-3 (TUA3), S-Adenosyl Methionine Decarboxylase (SAMDC), TIP41-like Family Protein (TIP41), (UDP)-glucose Pyrophosphorylase (UGPase), 18S ribosomal RNA (18S), Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Plasma Intrinsic Protein 2 (PIP2) and ACTIN(ACT)) were assessed to determine their expression stability in 23 samples from different tissues or organs of loquat. Integrated expression stability evaluations using five computational statistical methods (GeNorm, NormFinder, ΔCt, BestKeeper, and RefFinder) suggested that a RG set, including RPL4, RPL18, HIS3 and TUA3, was the most stable one across all of the tested loquat samples. The expression pattern of EjCDKB1;2 in the tested loquat tissues normalized to the selected RG set demonstrated its reliability.ConclusionsThis study reveals the reliable RGs for accurate normalization of gene expression in loquat. In addition, our findings demonstrate an efficient system for identifying the most effective RGs for different organs, which may be applied to related rosaceous crops.Electronic supplementary materialThe online version of this article (10.1186/s13007-019-0391-2) contains supplementary material, which is available to authorized users.

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“…However, the ovule of its fruit becomes yellow and sheds at − 3 °C, resulting in heavy losses [4]. Therefore, breeding freezing-resistant varieties is important for the industrial development of loquat [1,5,6].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional reprogramming of the bud-mutation loquat YongLu emerging freezing resistant

Chen

Wang

et al. 2020

Preprint

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Background: Freezing seriously affects loquat, an originally subtropical fruit. Here, a wide-spread cultivar loquat bud mutation ‘Yonglu’ (YL) was found to be more resistant to freezing than the parental ‘Ruantiaobaisha’ (RT), especially the fruits. The freezing resistance mechanism of YL was analyzed by physiological and transcriptomic methods. Results: After freezing treatment, only the fruits of YL showed a significant accumulation of proline (which increased by 1.5-fold). Interestingly, abscisic acid (ABA) accumulation in YL showed no difference after freezing treatment. Moreover, the stomatal density, area, and apertures of YL significantly decreased. All these results suggested that the proline content and stomatal closure contributed to the enhanced freezing tolerance. Further, the transcriptional profiles of freezing tissues were obtained and compared. Functional enrichment analysis showed that aside from basic nutrients and energy metabolisms, such as citrate cycle and oxidative phosphorylation, the fruits and flowers of YL have different systems to enhance freezing tolerance, suggesting a progressive freezing-resistant network. Signaling transduction of nearly all hormones, including ABA, was significantly differentially expressed. Several key genes, such as PP2C and SnRK2, which are crucial for transducing ABA signals, were significantly down- and upregulated, respectively. But the genes of neither ABA biosynthesis nor ABA transduction pathway significantly differentially expressed between two freezing varieties, suggesting they contribute limit in enhancing freezing tolerance of YL. Conclusion: Concludingly, the bud mutation YL loquat has stronger freezing tolerance than its parental RT due to the increasing proline and more effective regulation of stomatal closure. Furthermore, the bud mutation YL loquat reprogrammed its entire transcriptional profiles to enhance their abilities to adapt to freezing stress. However, ABA biosynthesis, ABA transduction pathway and CBF pathway, which are common reasons in cold response, were not the main reason for the increasing freezing tolerance of YL. There must be unknown regulation mechanisms for the bud mutation to freezing resistant.

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Loquat (Eriobotrya Lindl.)

Cited by 14 publications

References 25 publications

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

Over-expression of EjLFY-1 Leads to an Early Flowering Habit in Strawberry (Fragaria × ananassa) and Its Asexual Progeny

Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Transcriptional reprogramming of the bud-mutation loquat YongLu emerging freezing resistant

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