Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing

Aguilar, Dora Trejo; Lara-Capistrán, Liliana; Maldonado-Mendoza, Ignacio E.; Zulueta-Rodríguez, Ramón; Sangabriel-Conde, Wendy; Mancera-López, María Elena; Negrete‐Yankelevich, Simoneta; Barois, Isabelle

doi:10.5598/imafungus.2013.04.02.01

Cited by 31 publications

(31 citation statements)

References 36 publications

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“…Importantly, A. colombiana was dominant in all three study areas and was persistently found and easily produced in trap culture. Abundance and persistence of AMF species are very important for efficient AMF species selection to ensure potential inocula are not lost during trap culture propagation (Trejo-Aguilar et al, 2013). This is essential, as the most widespread method for inoculum propagation is the use of trap plants (Berruti et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Selecting Native Arbuscular Mycorrhizal Fungi to Promote Cassava Growth and Increase Yield under Field Conditions

et al. 2016

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The use of arbuscular mycorrhizal fungal (AMF) inoculation in sustainable agriculture is now widespread worldwide. Although the use of inoculants consisting of native AMF is highly recommended as an alternative to commercial ones, there is no strategy to allow the selection of efficient fungal species from natural communities. The objective of this study was (i) to select efficient native AMF species (ii) evaluate their impact on nematode and water stresses, and (iii) evaluate their impact on cassava yield, an important food security crop in tropical and subtropical regions. Firstly, native AMF communities associated with cassava rhizospheres in fields were collected from different areas and 7 AMF species were selected, based upon their ubiquity and abundance. Using these criteria, two morphotypes (LBVM01 and LBVM02) out of the seven AMF species selected were persistently dominant when cassava was used as a trap plant. LBVM01 and LBVM02 were identified as Acaulospora colombiana (most abundant) and Ambispora appendicula, respectively, after phylogenetic analyses of LSU-ITS-SSU PCR amplified products. Secondly, the potential of these two native AMF species to promote growth and enhance tolerance to root-knot nematode and water stresses of cassava (Yavo variety) was evaluated using single and dual inoculation in greenhouse conditions. Of the two AMF species, it was shown that A. colombiana significantly improved the growth of the cassava and enhanced tolerance to water stress. However, both A. colombiana and A. appendicula conferred bioprotective effects to cassava plants against the nematode Meloidogyne spp., ranging from resistance (suppression or reduction of the nematode reproduction) or tolerance (low or no suppression in cassava growth). Thirdly, the potential of these selected native AMF to improve cassava growth and yield was evaluated under field conditions, compared to a commercial inoculant. In these conditions, the A. colombiana single inoculation and the dual inoculation significantly improved cassava yield compared to the commercial inoculant. This is the first report on native AMF species exhibiting multiple benefits for cassava crop productivity, namely improved plant growth and yield, water stress tolerance and nematode resistance.

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Section: Discussionmentioning

confidence: 99%

Selecting Native Arbuscular Mycorrhizal Fungi to Promote Cassava Growth and Increase Yield under Field Conditions

et al. 2016

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“…One of the common practices to maintain the propagated spores is trap culture method. The use of single host for several plant cycles reduce the diversity of AMF spores (Trejo-Aguilar et al, 2013), however, plant rotation from C3 to C4 or vice versa may promote diverse AMF species (INVAM -International Culture Collection of (Vesicular) Arbuscular Mycorrhizal fungi). In the present study, we used two different host plants which were commonly used for AMF propagation.…”

Section: Effect Of Mycorrhizal Inoculation On the Growth Parametersmentioning

confidence: 99%

“…In addition, there is no proper procedure for long-term storage of this propagated AMF. Trejo-Aguilar et al (2013) reported that long-term subculturing reduced the diversity of AMF spores in trap culture when a single host was used. During continuous propagation cycle, dilution of this trap culture inoculum may favor the diverse AMF species than the field (Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Selvakumar¹,

Kim²,

Walitang³

et al. 2016

Korean Journal of Soil Science and Fertilizer

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Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

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“…Trejo‐Aguilar et al . () found that utilization of multiple AMF species from different families to initiate spore production displayed better results; however, certain AMF species, especially from Glomeraceae family were predominantly present in the produced AMF culture than other AMF species. These reports are a clear indication of the myriad number of players involved in the generation of pure AMF cultures.…”

Section: Discussionmentioning

confidence: 99%

“…In a study by Gryndler et al (2003), it was reported that the frequency of root colonization, arbuscules abundance and growth of extraradical mycelium decreased after 11 months of cultivation. Trejo-Aguilar et al (2013) found that utilization of multiple AMF species from different families to initiate spore production displayed better results; however, certain AMF species, especially from Glomeraceae family were predominantly present in the produced AMF culture than other AMF species. These reports are a clear indication of the myriad number of players involved in the generation of pure AMF cultures.…”

Section: Gigaspora Margarita T-42 (Kp677602)mentioning

confidence: 94%

Arbuscular mycorrhizal fungi spore propagation using single spore as starter inoculum and a plant host

et al. 2018

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Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing

Cited by 31 publications

References 36 publications

Selecting Native Arbuscular Mycorrhizal Fungi to Promote Cassava Growth and Increase Yield under Field Conditions

Selecting Native Arbuscular Mycorrhizal Fungi to Promote Cassava Growth and Increase Yield under Field Conditions

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Arbuscular mycorrhizal fungi spore propagation using single spore as starter inoculum and a plant host

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