2021
DOI: 10.1177/19476035211044820
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Loss of Autophagy Causes Increased Apoptosis of Tibial Plateau Chondrocytes in Guinea Pigs with Spontaneous Osteoarthritis

Abstract: Objective The goal of the present study was to observe the effect of autophagy in tibial plateau chondrocytes on apoptosis in spontaneous knee osteoarthritis (OA) in guinea pigs. Design Fifty 2-month-old female Hartley guinea pigs were divided into a normal group (10 animals, all euthanized after 7 months) and an OA group (40 animals, 10 of which were euthanized after 10 months). Immunohistochemistry, RT-qPCR and Western blotting were used to evaluate autophagy levels, intracellular glycogen accumulation and a… Show more

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Cited by 5 publications
(2 citation statements)
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“…The i.a. injections of Rapa after 1 month reduced the OARSI score MMP13, Glycogenin 1, and Tunel, and increased aggrecan and Beclin 1 compared to 3-methyladenine (3-MA) and saline solution [40].…”
Section: Injective Treatmentsmentioning
confidence: 95%
“…The i.a. injections of Rapa after 1 month reduced the OARSI score MMP13, Glycogenin 1, and Tunel, and increased aggrecan and Beclin 1 compared to 3-methyladenine (3-MA) and saline solution [40].…”
Section: Injective Treatmentsmentioning
confidence: 95%
“…Chondrocyte proliferation, differentiation, hypertrophy, and ECM formation are essential for skeletal development and linear growth (Berendsen and Olsen, 2015). Autophagy is required for the conversion of mesenchymal stem cells to proliferating chondrocytes, as reflected by the completion of a multi-step differentiation process from mesenchymal condensation to calcification during normal ATDC5 cell culture, whereas COL2A1 expression is completely blocked during the culture of Atg7-deficient ATDC5 cells (Wang X. J. et al, 2021). Even if the transformation can be completed, deletion of Atg5 or Atg7 in the chondrocyte will also result in decreased chondrocyte proliferation and higher apoptosis, as well as related growth retardation and shorter bone length in mice (Vuppalapati et al, 2015;Wang X. J. et al, 2021).…”
Section: Autophagy In Chondrocytesmentioning
confidence: 99%