Loss of Cep72 affects the morphology of spermatozoa in mice

Chen, Zhe; Xu, Yating; Ma, Dupeng; Li, Changrong; Yu, Ziqi; Liu, Cong; Jin, Tingyu; Du, Ziye; Li, Zejia; Sun, Qi; Xu, Youyong; Liu, Rong; Wu, Yuerong; Luo, Mengcheng

doi:10.3389/fphys.2022.948965

Front. Physiol.

2022

DOI: 10.3389/fphys.2022.948965

|View full text |Cite

Loss of Cep72 affects the morphology of spermatozoa in mice

Zhe Chen

Yating Xu²,

Dupeng Ma³

et al.

Abstract: The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although Cep72 knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

Sadamitsu,

Velilla,

Shinya

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Inbred strains of organisms are genetically highly uniform and thus useful for life science research. We have previously reported the ongoing generation of the zebrafish IM strain from the India (IND) strain through full sib-pair mating for 16 generations. However, the IM fish laid a small number of offspring and had a short lifespan, implying the need for discreet care in breeding. Here, we report the subsequent establishment of IM strain as well as the generation of a new inbred zebrafish strain, Mishima-AB (M-AB). M-AB was derived from the *AB strain by full sib-pair mating for over 20 generations, which fulfills the general criterion for the establishment of an inbred strain. In contrast to the IM case, maintenance of the M-AB strain by sib-pair mating required almost no special handling. Genome sequencing of IM individuals from the 47th generation and M-AB individuals from the 27th generation revealed that SNP-based genomic heterogeneity across whole-genome nucleotides was 0.008% and 0.011%, respectively. These percentages were much lower than those of the parental IND (0.197%) and *AB (0.086%) strains. These results indicate that the genomes of these inbred strains were highly homogenous. We also demonstrated the successful microinjection of antisense morpholinos, CRISPR/Cas9, and foreign genes into M-AB embryos at the 1-cell stage. Overall, we report the establishment of a zebrafish inbred strain, M-AB, which is capable of regular breeding and genetic manipulation. This strain will be useful for the analysis of genetically susceptible phenotypes such as behaviors, microbiome features and drug susceptibility.

show abstract

Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

Sadamitsu,

Velilla,

Shinya

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

Impact of air pollution from different sources on sperm DNA methylation

Vozdova,

Kubickova,

Kopecka

et al. 2024

International Journal of Environmental Health Research

View full text Add to dashboard Cite

PWWP3A deficiency accelerates testicular senescence in aged mice

Chen,

Liu,

et al. 2024

Andrology

View full text Add to dashboard Cite

BackgroundThe PWWP domain‐containing proteins are involved in chromatin‐associated biological processes, including transcriptional regulation and DNA repair, and most of them are significant for gametogenesis and early embryonic development in mammals. PWWP3A, one of the PWWP domain proteins, is a reader of H3K36me2/H3K36me3 and a response factor to DNA damage. However, the physiological role of PWWP3A in spermatogenesis and fertility remains unclear.ObjectiveThe goal of this study was to explore the function and mechanism of PWWP3A in the process of spermatogenesis.Materials and methodsWe generated V5‐Pwwp3a KI mice and PWWP3A polyclonal antibody to observe the localization of PWWP3A in vivo. Meanwhile, Pwwp3a KO mice was used to explore the function in spermatogenesis.ResultsWe reported that PWWP3A is a predominant expression in the testis of mice. During spermatogenesis, PWWP3A exhibits the temporal expression from early‐pachytene to the round spermatids. The results of spermatocyte spreading and immunostaining showed that PWWP3A aggregated on the XY body, which then diffused as the XY chromosome separated at late‐diplotene. Although the depletion of PWWP3A had no obvious reproductive defects in young male mice, there were observed morphological abnormalities in sperm heads. Immunoprecipitation demonstrated the interaction of PWWP3A with DNA repair proteins SMC5/6; however, PWWP3A deficiency did not result in any meiotic defects. Notably, the testes of aged male Pwwp3a KO mice displayed pronounced degeneration, and were characterized by the presence of vacuolated seminiferous tubules. Furthermore, RNA‐seq analysis revealed an upregulation in the expression of genes which may be involving in immunoregulatory and inflammatory response pathways in aged Pwwp3a KO mice with testicular degeneration.ConclusionsOur study showed that PWWP3A was highly enriched in the mouse testis, and the Pwwp3a KO mice were fertile. However, the aged Pwwp3a KO male mice displayed testicular atrophy that may be due to changes in the immune micro‐environment or abnormal repair of DNA damage.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Loss of Cep72 affects the morphology of spermatozoa in mice

Cited by 3 publications

References 41 publications

Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

Impact of air pollution from different sources on sperm DNA methylation

PWWP3A deficiency accelerates testicular senescence in aged mice

Contact Info

Product

Resources

About