Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection

Maenaka, Shiori; Hikichi, Tetsurou; Imai, Miwa; Minamoto, Toshinari; Kawahara, Ei

doi:10.3892/or.15.4.791

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…In support of our finding, other investigators have recently reported a high percentage of LOI in CRCs (54%) using laser microdissection [47]. Contamination of the tumor tissues with normal cells may result in underestimation of the percentage of LOI.…”

Section: Discussionsupporting

confidence: 75%

Genetic alterations in colorectal cancers with demethylation of insulin-like growth factor II

et al. 2008

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Section: Discussionsupporting

confidence: 75%

Genetic alterations in colorectal cancers with demethylation of insulin-like growth factor II

et al. 2008

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“…Our technique, which has been modified from previous approaches [10], [11], provides a generalizable strategy for identifying cell class-specific imprinted genes. Such studies can help bring resolution to the important debate about the number and identity of imprinted genes in the brain [7]–[9].…”

Section: Discussionmentioning

confidence: 99%

Snx14 Regulates Neuronal Excitability, Promotes Synaptic Transmission, and Is Imprinted in the Brain of Mice

et al. 2014

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Genomic imprinting describes an epigenetic process through which genes can be expressed in a parent-of-origin-specific manner. The monoallelic expression of imprinted genes renders them particularly susceptible to disease causing mutations. A large proportion of imprinted genes are expressed in the brain, but little is known about their functions. Indeed, it has proven difficult to identify cell type-specific imprinted genes due to the heterogeneity of cell types within the brain. Here we used laser capture microdissection of visual cortical neurons and found evidence that sorting nexin 14 (Snx14) is a neuronally imprinted gene in mice. SNX14 protein levels are high in the brain and progressively increase during neuronal development and maturation. Snx14 knockdown reduces intrinsic excitability and severely impairs both excitatory and inhibitory synaptic transmission. These data reveal a role for monoallelic Snx14 expression in maintaining normal neuronal excitability and synaptic transmission.

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“…Additionally, positive staining for IGF‐II correlates with increased tumor progression, increased proliferating cell nuclear antigen staining, and decreased patient survival in colorectal cancers (Wang et al . 2005; Maenaka et al . 2006).…”

Section: Discussionmentioning

confidence: 99%

Secreted CREG inhibits cell proliferation mediated by mannose 6‐phosphate/insulin‐like growth factor II receptor in NIH3T3 fibroblasts

et al. 2008

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Cellular repressor of E1A‐stimulated genes (CREG) is a recently described glycoprotein that plays a critical role in keeping cells or tissues in mature, homeostatic states. To understand the relationship between CREG and its membrane receptor, mannose 6‐phosphate/insulin‐like growth factor II receptor (M6P/IGF2R), we first generated stable NIH3T3 fibroblasts by transfection of pDS_shCREGs vectors, which produced an approximately 80% decrease in CREG levels both in the lysate and in the media. We used fluorescence activated cell sorting and a bromide deoxyuridine incorporation assay to identify whether CREG knockdown promoted the cell proliferation associated with the increase of IGF‐II in NIH3T3 fibroblasts. Proliferation was markedly inhibited in a concentration‐dependent manner by re‐addition of recombinant CREG protein into the media, and this was mediated by the membrane receptor M6P/IGF2R. We subsequently confirmed the direct interaction of CREG and M6P/IGF2R by both immunoprecipitation‐Western blotting and immunofluorescence staining. We found that expression of CREG correlated with localization of the receptor in NIH3T3 fibroblasts but did not affect its expression. Our findings indicated that CREG might act as a functional regulator of M6P/IGF2R to facilitate binding and trafficking of IGF‐II endocytosis, leading to growth inhibition.

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Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection

Cited by 10 publications

References 22 publications

Genetic alterations in colorectal cancers with demethylation of insulin-like growth factor II

Genetic alterations in colorectal cancers with demethylation of insulin-like growth factor II

Snx14 Regulates Neuronal Excitability, Promotes Synaptic Transmission, and Is Imprinted in the Brain of Mice

Secreted CREG inhibits cell proliferation mediated by mannose 6‐phosphate/insulin‐like growth factor II receptor in NIH3T3 fibroblasts

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