Loss of O157 O Antigenicity of Verotoxin-Producing
            <i>Escherichia coli</i>
            O157:H7 Surviving under Starvation Conditions

Hara-Kudo, Yukiko; Miyahara, Makoto; Kumagai, Shinya

doi:10.1128/aem.66.12.5540-5543.2000

Cited by 23 publications

(15 citation statements)

References 16 publications

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“…Moreover, enterobacteria exposed to LSMMG appear to have enhanced stress resistance (Lynch et al 2004) and other stress conditions have been known to affect antibodybased detection of bacteria (Hara-Kudo et al 2000;Shelton and Karns 2001;Padhye and Doyle 1991;Geng et al 2006). In contrast to the present work, loss of O-antigenicity, measured by agglutination, was observed when E. coli O157:H7 was grown in starvation conditions for months (Hara-Kudo et al 2000). Most importantly, the lost Oantigenicity was not recovered by culture in TSB.…”

Section: Discussioncontrasting

confidence: 84%

“…The finding that growth in LSMMG did not significantly alter ELISA detection of Salmonella Typhimurium was surprising because LSMMG, produced in the same manner and for the same time period as the present paper, changed Salmonella Typhimurium LPS gene expression resulting in a significant loss of LPS molecules per bacterium and loss of O-antigen (Wilson et al 2002;Nickerson et al 2003). Moreover, enterobacteria exposed to LSMMG appear to have enhanced stress resistance (Lynch et al 2004) and other stress conditions have been known to affect antibodybased detection of bacteria (Hara-Kudo et al 2000;Shelton and Karns 2001;Padhye and Doyle 1991;Geng et al 2006). In contrast to the present work, loss of O-antigenicity, measured by agglutination, was observed when E. coli O157:H7 was grown in starvation conditions for months (Hara-Kudo et al 2000).…”

Section: Discussioncontrasting

confidence: 51%

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Antibody-based Detection of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium Grown in Low-shear Modeled Microgravity

Nyquist-Battie

Freeman

Leckband

et al. 2007

Microgravity Sci. Technol

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With the advent of prolonged spaceflights, it is important to determine if antibody-based assays can be used to monitor food and water for bacterial contaminants. In the present work, a ground-based high aspect ratio vessel (HARV) was used to determine if low shear modeled microgravity (LSMMG) alters antibody-binding to E. coli O157:H7 and Salmonella enterica serovar Typhimurium. Antibody-bacteria binding was similar under LSMMG and normal gravity because there was no difference in amount of captured bacteria measured by colony forming units (CFU) between assays conducted in the HARV and a conventional roller flask. The ability of E. coli O157:H7 and Salmonella Typhimurium grown in LSMMG to bind specific antibodies was also studied. After incubations of 4, 18 or 36 h in the HARV or a shaking incubator, bacteria were harvested for enzyme-linked immunosorbent assays (ELISA). In the E. coli O157:H7 ELISA using a goat polyclonal primary antibody, LSMMG did not alter the linear range of detection (10 5 -10 7 cells/ml) nor the signal to noise ratio at any bacterial concentration. Although insignificant changes in signal to noise ratios were evident, LSMMG did not alter the range of detection (10 5 -10 7 cells/ml) for Salmonella Typhimurium in ELISAs using either a polyclonal or a monoclonal antibody. These results suggest that immunoassays may be used in spacecrafts because LSMMG does not have significant deleterious effects on antibody-binding to bacteria nor does it significantly alter surface antigens necessary for antibody-based methods.

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Section: Discussioncontrasting

confidence: 84%

Section: Discussioncontrasting

confidence: 51%

Antibody-based Detection of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium Grown in Low-shear Modeled Microgravity

Nyquist-Battie

Freeman

Leckband

et al. 2007

Microgravity Sci. Technol

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“…Starvation of E. coli O157:H7 in water has, however, been shown to influence the expression of the O157 antigen (12) and the development of a chlorine-resistant phenotype (20). Other studies also point to the importance of the physiological condition of E. coli O157:H7 with regard to the expression of cellular adhesion factors (13).…”

Section: Discussionmentioning

confidence: 99%

A Stable Bioluminescent Construct of Escherichia coli O157:H7 for Hazard Assessments of Long-Term Survival in the Environment

Ritchie

Campbell

Shepherd

et al. 2003

Appl Environ Microbiol

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A chromosomally lux-marked (Tn5 luxCDABE) strain of nontoxigenic Escherichia coli O157:H7 was constructed by transposon mutagenesis and shown to have retained the O157, H7, and intimin phenotypes. The survival characteristics of this strain in the experiments performed (soil at ؊5, ؊100, and ؊1,500 kPa matric potential and artificial groundwater) were indistinguishable from the wild-type strain. Evaluation of potential luminescence was found to be a rapid, cheap, and quantitative measure of viable E. coli O157:H7 Tn5 luxCDABE populations in environmental samples. In the survival studies, bioluminescence of the starved populations of E. coli O157:H7 Tn5 luxCDABE could be reactivated to the original levels of light emission, suggesting that these populations remain viable and potentially infective to humans. The attributes of the construct offer a cheap and low-risk substitute to the use of verocytotoxin-producing E. coli O157:H7 in long-term survival studies.

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“…Viable cell counts were performed at days 0, 7, 14, 21, and 28 as described above. DW was used, because the population of STEC decreases very slowly in other nutrient-depleted media, such as phosphate-buffered saline (11), and it is not appropriate to measure an inactivation rate. Therefore, this assay evaluated the bacterial response against starvation in the environment as well as low osmotic pressure.…”

Section: Methodsmentioning

confidence: 99%

Variation in Stress Resistance Patterns among stx Genotypes and Genetic Lineages of Shiga Toxin-Producing Escherichia coli O157

Lee

French

Jones

et al. 2012

Appl Environ Microbiol

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ABSTRACTTo evaluate the relationship between bacterial genotypes and stress resistance patterns, we exposed 57 strains of Shiga toxin-producingEscherichia coli(STEC) O157 to acid, freeze-thaw, heat, osmotic, oxidative, and starvation stresses. Inactivation rates were calculated in each assay and subjected to univariate and multivariate analyses, including principal component analysis (PCA) and cluster analysis. Thestxgenotype was determined for each strain as was the lineage-specific polymorphism assay (LSPA6) genotype. In univariate analyses, strains of thestx1stx2genotype showed greater resistance to heat than strains of thestx1stx2cgenotype; moreover, strains of thestx1stx2genotype showed greater resistance to starvation than strains of thestx2orstx2cgenotypes. LSPA6 lineage I (LI) strains showed greater resistance to heat and starvation than LSPA6 lineage II (LII) strains. PCA revealed a general trend that a strain with greater resistance to one type of stress tended to have greater resistance to other types of stresses. In cluster analysis, STEC O157 strains were grouped into stress-resistant, stress-sensitive, and intermediate clusters. Instxgenotypes, all strains of thestx1stx2genotype were grouped with the stress-resistant cluster, whereas 72.7% (8/11) of strains of thestx1stx2cgenotype grouped with the stress-sensitive cluster. In LI strains, 77.8% (14/18) of the strains were grouped with the stress-resistant cluster, whereas 64.7% (11/17) of LII strains were grouped with the stress-sensitive cluster. These results indicate that the genotypes of STEC O157 that are frequently associated with human illness, i.e., LI or thestx1stx2genotype, have greater multiple stress resistance than do strains of other genotypes.

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Loss of O157 O Antigenicity of Verotoxin-Producing Escherichia coli O157:H7 Surviving under Starvation Conditions

Cited by 23 publications

References 16 publications

Antibody-based Detection of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium Grown in Low-shear Modeled Microgravity

Antibody-based Detection of Escherichia coli O157:H7 and Salmonella enterica Serovar Typhimurium Grown in Low-shear Modeled Microgravity

A Stable Bioluminescent Construct of Escherichia coli O157:H7 for Hazard Assessments of Long-Term Survival in the Environment

Variation in Stress Resistance Patterns among stx Genotypes and Genetic Lineages of Shiga Toxin-Producing Escherichia coli O157

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