Loss of Peptides and Proteins Upon Sterile Filtration Due to Adsorption to Membrane Filters

Oetelaar, Piet J.M. van den; Mentink, Ingrid M.; Brinks, G.J.

doi:10.3109/03639048909040198

Cited by 19 publications

(5 citation statements)

References 5 publications

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“…Throughput and protein uptake initially increased proportionally. Interestingly, protein adsorption/deposition on the membrane remained nearly constant after a certain throughput, showing a plateau for all protein concentrations, similar to results obtained previously by van den Oetelaar et al, [13] with a slight variation between the different protein concentrations. Higher concentrations of protein (i.e., 100 g L −1 ) showed a greater amount of protein uptake as expected.…”

Section: Sterile Filtration For Final Filingsupporting

confidence: 89%

“…Most studies of protein adsorption have been performed in static conditions by immersing the membrane in the protein solution, which may not be representative of the conditions encountered during the filtration process. [11,12] Van den Oetelaar et al [13] evaluated the protein losses on five types of sterile filters during both static adsorption and filtration conditions. Adsorptive losses of BSA and IgG were less than 2 mg (for a 47 mm diameter disk) when filters were simply soaked in 1 mg mL −1 of solutions of the protein.…”

Section: Manufacturer Membranementioning

confidence: 99%

“…[ 11,12 ] Van den Oetelaar et al. [ 13 ] evaluated the protein losses on five types of sterile filters during both static adsorption and filtration conditions. Adsorptive losses of BSA and IgG were less than 2 mg (for a 47 mm diameter disk) when filters were simply soaked in 1 mg mL −1 of solutions of the protein.…”

Section: Introductionmentioning

confidence: 99%

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Protein loss during membrane processes in biopharmaceutical manufacturing

Na,

Behboudi,

Mun

et al. 2024

Biotechnology Journal

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Maximizing product yield in biopharmaceutical manufacturing processes is a critical factor in determining the overall cost of goods, especially given the high value of these biological products. However, there has been relatively limited research on the quantitative analysis of protein losses due to adsorption and fouling during the different membrane filtration processes employed in typical downstream operations. This study aims to provide a comprehensive analysis of protein loss in the range of membrane systems used in downstream processing including clarification, virus removal filtration, ultrafiltration/diafiltration for formulation, and final sterile filtration, all using commercially available membranes with three model proteins (bovine serum albumin, human serum albumin, and immunoglobulin G). The correlation between protein loss and various parameters (i.e., protein type, protein concentration, throughput, membrane morphology, and protein removal mechanism) was also investigated. This study provides important insights into the nature of protein loss during membrane processes as well as a methodology for quantifying protein yield loss in bioprocesses.

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Section: Sterile Filtration For Final Filingsupporting

confidence: 89%

Section: Manufacturer Membranementioning

confidence: 99%

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Protein loss during membrane processes in biopharmaceutical manufacturing

Na,

Behboudi,

Mun

et al. 2024

Biotechnology Journal

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“…The subsequent binding of analyte to the capture layer can be quantitatively investigated by many of the techniques to be discussed here. The deposition of proteins on electron microscope grids, and the screening of materials for affinity chromatography (Kopaciewicz, 1983;Place et al 1991), are further fields in which a quantitative knowledge of the kinetics of protein adsorption is indispensible for obtaining deeper insight; others are biocompatibility in medicine, which, with the growing use of artificial implants, internal organs and in vivo sensors is growing in importance, and the fouling of filters used in ultrafiltration and other techniques of protein purification (van den Oetelaar et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Experimental methods for investigating protein adsorption kinetics at surfaces

Ramsden¹

1994

Quart. Rev. Biophys.

177

131

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The adsorption of proteins at the solid-liquid interface is a process of fundamental importance in nature. Extensive reviews (MacRitchie, 1978; Andrade & Hlady, 1986; Norde, 1986) testify to the strong interest which has been shown in the problem during the past few decades. Norde & Lyklema (1978) have rightly pointed out that protein adsorption is scientifically intriguing; the phenomenology is complicated and includes many presently apparently irreconcilable observations.

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“…To maintain protein activity and function, an undamaged sustainment of the 3-D protein structure is obligatory. Batch processing of HuFF included sterile filtration that can have a negative impact on protein quality and quantity [ 121 , 122 ]. To minimize further negative effects, HuFF samples were frozen in 1 ml aliquots to avoid repeated freezing thawing cycles and stored at − 196 °C in liquid nitrogen to keep protein degradation at a low level.…”

Section: Limitationsmentioning

confidence: 99%

In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions

Schallmoser

Einenkel

Färber

et al. 2022

Arch Gynecol Obstet

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Background Despite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer subtypes may lead to the transfer of malignant cells when transplantation of affected ovarian tissue is conducted. IVG and subsequent isolation of vital follicles obtained from frozen thawed ovarian tissue for further in vitro maturation (IVM) would expand current fertility protection techniques while reducing the risk of retransplanting malignant cells. Methods A total of 216 cortical biopsies from 3 patients were included in this study in 4 treatment groups. After freezing, thawing and 8 days of hypoxic tissue culture supplemented with different concentrations of human follicular fluid (HuFF) and follicle-stimulating hormone (FSH), follicles were isolated enzymatically and stained with calcein to determine follicular viability. Numbers and size of vital follicles were assessed by fluorescence microscopy (Ti2, Nikon) and specified by computer assisted, semi-automated measurement (NIS software, Nikon). To estimate the effect of in vitro culture on apoptosis, tissue sections were stained for nicked DNA (TUNEL) prior and after tissue culture. Results Analysing 3025 vital follicles, we observed significant differences [P < 0.01] regarding follicle size when hypoxic tissue culture was supplemented with HuFF compared with the control group on day 1, individual follicles reached sizes > 100 µm. Conclusions The results implicate that HuFF contains valuable factors contributing to significant IVG of follicles in human ovarian tissue and could be regarded as an additional tool in personalized fertility restoration prior to retransplantation of ovarian tissue.

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Loss of Peptides and Proteins Upon Sterile Filtration Due to Adsorption to Membrane Filters

Cited by 19 publications

References 5 publications

Protein loss during membrane processes in biopharmaceutical manufacturing

Protein loss during membrane processes in biopharmaceutical manufacturing

Experimental methods for investigating protein adsorption kinetics at surfaces

In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions

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