2007
DOI: 10.1074/jbc.m610249200
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Loss of Phylloquinone in Chlamydomonas Affects Plastoquinone Pool Size and Photosystem II Synthesis

Abstract: Phylloquinone functions as the electron transfer cofactor at the A 1 site of photosystem I. We have isolated and characterized a mutant of Chlamydomonas reinhardtii, menD1, that is deficient in MenD, which encodes 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, an enzyme that catalyzes the first specific step of the phylloquinone biosynthetic pathway. The mutant is photosynthetically active but light-sensitive. Analysis of total pigments by mass spectrometry reveals that phylloquinone is absent… Show more

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Cited by 37 publications
(45 citation statements)
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“…PQ, on the other hand, was shown to partially complement the lack of phylloquinone in photosystem I of Synechocystis sp. and Chlamydomonas reinhardtii but not in Arabidopsis thaliana (42)(43)(44).…”
Section: Discussionmentioning
confidence: 99%
“…PQ, on the other hand, was shown to partially complement the lack of phylloquinone in photosystem I of Synechocystis sp. and Chlamydomonas reinhardtii but not in Arabidopsis thaliana (42)(43)(44).…”
Section: Discussionmentioning
confidence: 99%
“…Among the mutants identified, some affect state transitions indirectly. This is the case for stt10 and stt2, which are deficient in the expression of MenC and MenD, two genes involved in the phylloquinone biosynthetic pathway [28]. In at least one of these mutants, phylloquinone, which normally acts as an electron carrier within PSI, is replaced by plastoquinone.…”
Section: P700mentioning
confidence: 99%
“…Chorismate and 2-oxoglutarate serve as precursors for the formation of the naphthalenoid ring, which is prenylated and then methylated. Phylloquinone biosynthetic mutants in the cyanobacterium Synechocystis sp PCC 6803 and the green alga Chlamydomonas reinhardtii are still able to grow photoautotrophically, for these species recruit plastoquinone-9 in place of phylloquinone at the A 1 site of photosystem I (Semenov et al, 2000;Lefebvre-Legendre et al, 2007). By contrast, loss of phylloquinone biosynthesis in Arabidopsis thaliana results in seedling lethal phenotype (van Oostende et al, 2011).…”
Section: Introductionmentioning
confidence: 99%