Low Concentrations of Acid-Soluble Thiol (Cysteine) in the Blood Plasma of HIV-1-Infected Patients

Eck, Hans‐Peter; Gmünder, Helmut; Hartmann, Martin; Petzoldt, D; Daniel, Volker; Dröge, Wulf

doi:10.1515/bchm3.1989.370.1.101

Cited by 325 publications

(150 citation statements)

References 10 publications

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“…Although it is possible that the isolated T cells can take up intact GSH, no evidence for this was found in studies on mouse peripheral lymphocytes in vivo (43), human lymphoid cell lines (31,41), and human peripheral lymphocytes (6,11 (45). Such patients have low levels of plasma cysteine and of GSH in blood mononuclear cells (46,47). The synthesis of GSH by peripheral lymphocytes seems to depend on transport of cysteine moieties.…”

Section: Resultsmentioning

confidence: 99%

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Suthanthiran

Anderson

Sharma

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

336

176

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Regulation of proliferation of normal human T lymphocytes (T cells) by glutathione (GSH) was explored with T-cell activation models that-do not require accessory cell signals. L-Buthionine-(S,R)-sulfoximine (BSO), which inactivates y-glutamylcysteine synthetase and therefore inhibits GSH synthesis, inhibited proliferation elicited by monoclonal antibodies directed at cluster designation 2 (CD2) and CD3

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Section: Resultsmentioning

confidence: 99%

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Suthanthiran

Anderson

Sharma

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

336

176

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“…The Nef protein is mainly localized in the cytoplasm (Franchini et al, 1986), where reducing conditions are prevalent. It was shown that, especially in T cells of HIV-infected patients, the reduced glutathione (GSH) level is very low (Eck et al, 1989;Buhl et al, 1989;Roederer et al, 1991) because of the reduced cysteine and elevated glutamate levels in the blood (Eck et al, 1989;Droge et al, 1988). The intracellular GSH level in these cells is dependent on the uptake of cysteine or cystine, for which a different transport system exists.…”

Section: Discussionmentioning

confidence: 99%

Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1

Kienzle

Freund

Kalbitzer

et al. 1993

European Journal of Biochemistry

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The nef genes, derived from two different human immunodeficiency-virus-type-1 (HIV-1) strains, were expressed in procaryotic cells (Escherichia coli) and in eucaryotic cells (insect cells infected with nef-containing baculovirus). The oligomerization of recombinant Nef protein was studied by NMR spectroscopy and immunoblotting under various experimental conditions. 1H-NMR spectroscopy shows that native folded protein has the tendency to polymerize under low-salt conditions. These oligomers become covalently linked by disulfide bonds after decreasing the reduction potential, a process which is fully reversible. Cross-linking studies with bis(sulfo-succinimidyl)suberate and alkylation with iodoacetic acid under non-reducing and reducing conditions document for the first time that Nef can also form homomeric structures including monomers, dimers, trimers and tetramers in cell lysates and intact cells. We found disulfide-linked as well as non-covalently associated oligomers. Since the Nef molecules are not exclusively found in the cytoplasm of HIV infected cells and since the reduced glutathione concentration in lymphocytes of virus infected persons is known to be unusually low, it might be possible that these Nef oligomers have a biological function in vivo as well

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“…This is supported by the observation that (i) HIV-1-infected individuals have abnormally low levels of antioxidant defences (Eck et al 1989), and (ii) HIV-1 gene activation and virus replication in human T-cells can be induced by reactive oxygen species such as hydrogen peroxide (LegrandPoels et al 1990). At the conference, results obtained both in vitro and in vivo were presented.…”

Section: Iron Oxidative Stress and Hiv-1 Transcriptionmentioning

confidence: 93%

Conference report: International Conference on HIV and iron, Brugge 1997

Boelaert

Piette

Weinberg

1997

Tropical Med Int Health

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Low Concentrations of Acid-Soluble Thiol (Cysteine) in the Blood Plasma of HIV-1-Infected Patients

Cited by 325 publications

References 10 publications

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens.

Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1

Conference report: International Conference on HIV and iron, Brugge 1997

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