Low concentrations of glycine inhibit photorespiration and enhance the net rate of photosynthesis in Caragana korshinskii

Kang, Tai; Wu, Hongdou; Lu, Baiyan; Luo, Xueli; Gong, Chunmei; Bai, Juan

doi:10.1007/s11099-017-0688-1

Cited by 15 publications

(7 citation statements)

References 36 publications

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“…To further explore the role of photorespiration in cucumber resistance to FOC infection, 0.2 mg/L isoniazid (INH) was used to specifically inhibit photorespiration by suppressing the conversion of glycine to serine (Ahammed et al, 2017; Kang et al, 2018). INH spraying significantly reduced the values of C i * and Γ* under both infected and uninfected conditions (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Nitrate mediated resistance against Fusarium infection in cucumber plants acts via photorespiration

Sun

et al. 2021

Plant Cell & Environment

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Fusarium wilt is one of the major biotic factors limiting cucumber (Cucumis sativus L.) growth and yield. The outcomes of cucumber‐Fusarium interactions can be influenced by the form of nitrogen nutrition (nitrate [NO3−] or ammonium [NH4+]); however, the physiological mechanisms of N‐regulated cucumber disease resistance are still largely unclear. Here, we investigated the relationship between nitrogen forms and cucumber resistance to Fusarium infection. Our results showed that on Fusarium infection, NO3− feeding decreased the levels of the fungal toxin, fusaric acid, leaf membrane oxidative, organelle damage and disease‐associated loss in photosynthesis. Metabolomic analysis and gas‐exchange measurements linked NO3− mediated plant defence with enhanced leaf photorespiration rates. Cucumber plants sprayed with the photorespiration inhibitor isoniazid were more susceptible to Fusarium and there was a negative correlation between photorespiration rate and leaf membrane injury. However, there were positive correlations between photorespiration rate, NO3− assimilation and the tricarboxylic acid (TCA) cycle. This provides a potential electron sink or the peroxisomal H2O2 catalysed by glycolate oxidase. We suggest that the NO3− nutrition enhanced cucumber resistance against Fusarium infection was associated with photorespiration. Our findings provide a novel insight into a mechanism involving the interaction of photorespiration with nitrogen forms to drive wider defence.

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Section: Resultsmentioning

confidence: 99%

Nitrate mediated resistance against Fusarium infection in cucumber plants acts via photorespiration

Sun

et al. 2021

Plant Cell & Environment

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“…The CA activity results showed that only GT-DEF transgenic plants exhibited higher activity than WT controls, whereas there was no significant difference in DEF, GT, and WT controls. Glycolate oxidase (GO) is a key enzyme in the oxidation pathway of glycolic acid, and its activity affects the rate of photorespiration (Long et al, 1994; Kang et al, 2018). The results of GO assay showed that the GO activity in DEF and GT-DEF transgenic plants was significantly lower than in WT controls, whereas the GO activity of GT transgenic plants was not significantly different from the control (Figure 8).…”

Section: Resultsmentioning

confidence: 99%

“…RuBisCO, the key enzyme for carbon fixation; RuBisCO activase (RCA), which regulates the activity of RuBisCO enzyme (Bi et al, 2017; Mcfarlane et al, 2018). Glycolate oxidase (GO), the key enzyme in the oxidation pathway of glycolic acid, which affects the rate of photorespiration (Long et al, 1994; Kang et al, 2018). The third true leaves of transgenic cucumber plants were selected for enzyme extraction.…”

Section: Methodsmentioning

confidence: 99%

Introduction of Exogenous Glycolate Catabolic Pathway Can Strongly Enhances Photosynthesis and Biomass Yield of Cucumber Grown in a Low-CO2 Environment

et al. 2019

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Carbon dioxide (CO 2 ) is very important for photosynthesis of green plants. CO 2 concentration in the atmosphere is relatively stable, but it drops sharply after sunrise due to the tightness of the greenhouse and the absorption of CO 2 by vegetable crops. Vegetables in greenhouses are chronically CO 2 starved. To investigate the feasibility of using genetic engineering to improve the photosynthesis and yield of greenhouse cucumber in a low CO 2 environment, five genes encoding glyoxylate carboligase (GCL), tartronic semialdehyde reductase (TSR), and glycolate dehydrogenase (GlcDH) in the glycolate catabolic pathway of Escherichia coli were partially or completely introduced into cucumber chloroplast. Both partial pathway by introducing GlcDH and full pathway expressing lines exhibited higher photosynthetic efficiency and biomass yield than wild-type (WT) controls in low CO 2 environments. Expression of partial pathway by introducing GlcDH increased net photosynthesis by 14.9% and biomass yield by 44.9%, whereas the expression of the full pathway increased seed yield by 33.4% and biomass yield by 59.0%. Photosynthesis, fluorescence parameters, and enzymatic measurements confirmed that the introduction of glycolate catabolic pathway increased the activity of photosynthetic carbon assimilation-related enzymes and reduced the activity of photorespiration-related enzymes in cucumber, thereby promoting the operation of Calvin cycle and resulting in higher net photosynthetic rate even in low CO 2 environments. This increase shows an improvement in the efficiency of the operation of the photosynthetic loop. However, the utilization of cucumber of low concentration CO 2 was not alleviated. This study demonstrated the feasibility of introducing the pathway of exogenous glycolate catabolic pathway to improve the photosynthetic and bio-yield of cucumber in a low CO 2 environment. These findings are of great significance for high photosynthetic efficiency breeding of greenhouse cucumber.

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“…Another support for this view came from the increased activity of GS, whose chloroplastic GS2 isoform is responsible for the re-assimilation of NH 4 + derived from photorespiration (Thomsen et al, 2014;Zhong et al, 2019). On the other hand, photorespiration increased the consumption of carbohydrates and consequently reduced the photosynthesis and growth of plants (Kang et al, 2018).…”

Section: Sulfur-regulated Trade-off Between Growth and Andrographolide Compound Accumulation Was Associated With The Balance In Carbon Anmentioning

confidence: 99%

Sulfur Regulates the Trade-Off Between Growth and Andrographolide Accumulation via Nitrogen Metabolism in Andrographis paniculata

et al. 2021

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Nitrogen (N) and sulfur (S) are essential mineral nutrients for plant growth and metabolism. Here, we investigated their interaction in plant growth and andrographolide accumulation in medicinal plant Andrographis paniculata grown at different N (4 and 8 mmol·L−1) and S concentration levels (0.1 and 2.4 mmol L−1). We found that increasing the S application rate enhanced the accumulation of andrographolide compounds (AGCs) in A. paniculata. Simultaneously, salicylic acid (SA) and gibberellic acid 4 (GA4) concentrations were increased but trehalose/trehalose 6-phosphate (Tre/Tre6P) concentrations were decreased by high S, suggesting that they were involved in the S-mediated accumulation of AGCs. However, S affected plant growth differentially at different N levels. Metabolite analysis revealed that high S induced increases in the tricarboxylic acid (TCA) cycle and photorespiration under low N conditions, which promoted N assimilation and S metabolism, and simultaneously increased carbohydrate consumption and inhibited plant growth. In contrast, high S reduced N and S concentrations in plants and promoted plant growth under high N conditions. Taken together, the results indicated that increasing the S application rate is an effective strategy to improve AGC accumulation in A. paniculata. Nevertheless, the interaction of N and S affected the trade-off between plant growth and AGC accumulation, in which N metabolism plays a key role.

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Low concentrations of glycine inhibit photorespiration and enhance the net rate of photosynthesis in Caragana korshinskii

Cited by 15 publications

References 36 publications

Nitrate mediated resistance against Fusarium infection in cucumber plants acts via photorespiration

Nitrate mediated resistance against Fusarium infection in cucumber plants acts via photorespiration

Introduction of Exogenous Glycolate Catabolic Pathway Can Strongly Enhances Photosynthesis and Biomass Yield of Cucumber Grown in a Low-CO2 Environment

Sulfur Regulates the Trade-Off Between Growth and Andrographolide Accumulation via Nitrogen Metabolism in Andrographis paniculata

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