Low-cost and highly efficient DNA biosensor for heavy metal ion using specific DNAzyme-modified microplate and portable glucometer-based detection mode

Zhang, Jin; Tang, Ying; Teng, Liumei; Lu, Minghua; Tang, Dianping

doi:10.1016/j.bios.2015.01.001

Cited by 51 publications

(16 citation statements)

References 36 publications

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“…Compared to antibodies, aptamers and DNAzymes have distinct advantages originating from their nucleic acid properties: 1) they can be synthesized in the test tubes at a much lower cost than that of antibodies; 2) they can be readily conjugated to many signal transducing elements, such as fluorophores, nanomaterials and enzymes at an exact location; 3) they are more stable than antibodies at room temperature and during storage; 4) unlike antibodies, they can be denatured during manufacturing process and storage and can regain binding or catalytic activity after renaturation at physiological condition before sensing applications; 5) since the DNA and RNA can be synthesized chemically, they have minimal batch-to-batch variations, and 6) they have been shown to bind small molecules such as metal ions much more selectively than those of antibodies. These properties make them excellent molecular recognition elements in developing BGM-based biosensors to quantify a variety of analytical targets, such as cocaine (Xiang and Lu, 2011, Yan et al, 2013, Hou et al, 2014, Zhou et al, 2014b), adenosine (Xiang and Lu, 2011, Liu et al, 2012), ATP (Yan et al, 2013, Hou et al, 2014), dopamine (Hun et al, 2015b), melamine (Gu et al, 2015), myoglobin (Wang et al, 2015a), platelet-derived growth factor-BB (PDGF-BB) (Ma et al, 2014), interferon-gamma (Xiang and Lu, 2011, Lan et al, 2015), uranium (UO 2 2+ ) (Xiang and Lu, 2011, 2013), and lead (Pb 2+ ) (Fu et al, 2013, Xiang and Lu, 2013, Liao and Li, 2014, Zhang et al, 2015a). …”

Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

“…Besides aptamers, metal ion-dependent DNAzymes have also been successfully transformed into BGM-based sensors for highly selective quantification metal ions (Xiang and Lu, 2011, Fu et al, 2013, Xiang and Lu, 2013, Liao and Li, 2014, Zhang et al, 2015a). By introducing a DNA-conjugated invertase to the substrate of DNAzyme via hybridization, Xiang and co-workers developed a general methodology using BGMs to detect metal ions (Xiang and Lu, 2013), including Pb 2+ and UO 2 2+ .…”

Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

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Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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Recent advances in mobile network and smartphones have provided an enormous opportunity for transforming in vitro diagnostics (IVD) from central labs to home or other points of care (POC). A major challenge to achieving the goal is a long time and high costs associated with developing POC IVD devices in mobile Health (mHealth). Instead of developing a new POC device for every new IVD target, we and others are taking advantage of decades of research, development, engineering and continuous improvement of the blood glucose meter (BGM), including those already integrated with smartphones, and transforming the BGM into a general healthcare meter for POC IVDs of a wide range of biomarkers, therapeutic drugs and other analytical targets. In this review, we summarize methods to transduce and amplify selective binding of targets by antibodies, DNA/RNA aptamers, DNAzyme/ribozymes and protein enzymes into signals such as glucose or NADH that can be measured by commercially available BGM, making it possible to adapt many clinical assays performed in central labs, such as immunoassays, aptamer/DNAzyme assays, molecular diagnostic assays, and enzymatic activity assays onto BGM platform for quantification of non-glucose targets for a wide variety of IVDs in mHealth.

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Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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show abstract

“…Based on the Enz-AuNP-DNA amplification strategy, Pb 2+ as low as 1.0 pM could be detected under the optimal conditions (Zhang et al, 2015a). …”

Section: Personal Glucose Metermentioning

confidence: 99%

A mini-review on functional nucleic acids-based heavy metal ion detection

Zhan

Wang

et al. 2016

Biosensors and Bioelectronics

148

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“…Tsekenis et al designed an RNA-cleaving DNAzyme formed by hybridizing a laser printed and surface sensor-immobilized 17-E strand with the 17-S DNA substrate which was cleaved on introduction of Pb 2+ to induce a surface stress measured by changes in the device capacitance [124]. Zhang et al reported a Pb 2+ sensor based on the personal glucometer [125]. They immobilized the RNAcleaving DNAzyme on a microplate that contains invertase-labeled ssDNA strands immobilized on AuNPs.…”

Section: Dnazymes In the Detection Of Heavy Metalsmentioning

confidence: 99%

Aptamer Utility in Sensor Platformsfor the Detection of Toxins and Heavy Metals

2017

J Toxins

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Nucleic acid research has expanded in recent years through the development of an in vitro methodology for the derivation of functional nucleic acids, capable of binding to specific targets with high specificity and sensitivity. These activities are similar to those of antibodies, yet the single-stranded oligonucleotide, called aptamer, can be readily created on a large-scale using solid-phase oligonucleotide synthesis. This relative ease of synthesis has made aptamers a highly versatile tool for the detection of important targets for applications in imaging, diagnostics and therapeutics. Aptamers are able to show high fidelity to specific targets due to their selective affinities. They undergo structural alteration and topological changes when they bind to specific targets and most sensors using aptamers today leverage on this characteristic to record a detectable signal. The use of aptamers in modern applications is vast and cannot possibly be covered in a single review. This review explicitly focuses on DNA aptamers used for detecting toxins and heavy metals, and outlines their utility in contemporary sensor designs, and their future prospects as analytical tools.

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Low-cost and highly efficient DNA biosensor for heavy metal ion using specific DNAzyme-modified microplate and portable glucometer-based detection mode

Cited by 51 publications

References 36 publications

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

A mini-review on functional nucleic acids-based heavy metal ion detection

Aptamer Utility in Sensor Platformsfor the Detection of Toxins and Heavy Metals

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