This work analyzes the life cycle environmental impacts of producing a particleboard based on cardoon fibers and a starch/chitosan adhesive from a “cradle-to-gate” perspective, considering the following life cycle steps: raw material production, adhesive preparation (component mixing and heating), cardoon fiber preparation (crushing and sieving), adhesive and fiber mixing, hot-pressing and final processing. The functional unit is a particleboard with the dimensions of 220 × 220 × 16 mm3. For the life cycle inventory, experimental data obtained from the production of particleboard on a pilot scale were used. The Aspen Plus V9 software was used to simulate the heating process in the manufacture of the biological adhesive and obtain the data associated with this stage. Portuguese or European conditions were considered for the background processes, using data from the EcoInvent V3.5 LCI database. The environmental impacts were quantified using the RECIPE methodology. To complement the study, the VOCs present in the panel were analyzed using the “active headspace” technique. The results show that for most of the environmental impact categories, energy consumption is dominant, followed by starch and chitosan production. Using fully renewable electricity produced in photovoltaic panels, instead of the Portuguese electricity mix, significantly reduces the impacts in most of the environmental impact categories, for example, the carbon footprint is reduced by 34%. Future studies will analyze how the environmental impacts can be further reduced, and how process scale-up may influence them.