Low Distortion Solvent Bonding of Microfluidic Chips

Ng, Shu Pei; Wiria, Florencia Edith; Tay, Nam Beng

doi:10.1016/j.proeng.2015.09.212

Cited by 27 publications

(18 citation statements)

References 8 publications

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“… 8 However, as a strong solvent could dissolve PMMA, microchannel clogging is a universal drawback of solvent bonding. 9 , 10 As an alternative, a solvent with a moderate dissolution rate was applied for bonding PMMA with the help of heat, and the overall process took more than 10 min. 8 In our previous studies, PMMAs were successfully bonded using 90% ethanol as a solvent and UV light, and the bond strength was approximately 6.17 MPa.…”

Section: Introductionmentioning

confidence: 99%

Rapid Fabrication of Poly(methyl methacrylate) Devices for Lab-on-a-Chip Applications Using Acetic Acid and UV Treatment

et al. 2020

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In the present study, we introduce a new approach for rapid bonding of poly(methyl methacrylate) (PMMA)-based microdevices using an acetic acid solvent with the assistance of UV irradiation. For the anticipated mechanism, acetic acid and UV irradiation induced free radicals on the PMMA surfaces, and acrylate monomers subsequently formed cross-links to create a permanent bonding between the PMMA substrates. PMMA devices effectively bonded within 30 s at a low pressure using clamps, and a clogging-free microchannel was achieved with the optimized 50% acetic acid. For surface characterizations, contact angle measurements and bonding performance analyses were conducted using predetermined acetic acid concentrations to optimize bonding conditions. In addition, the highest bond strength of bonded PMMA was approximately 11.75 MPa, which has not been reported before in the bonding of PMMA. A leak test was performed over 180 h to assess the robustness of the proposed method. Moreover, to promote the applicability of this bonding method, we tested two kinds of microfluidic device applications, including a cell culture-based device and a metal microelectrode-integrated device. The results showed that the cell culture-based application was highly biocompatible with the PMMA microdevices fabricated using an acetic acid solvent. Moreover, the low pressure required during the bonding process supported the integration of metal microelectrodes with the PMMA microdevice without any damage to the metal films. This novel bonding method holds great potential in the ecofriendly and rapid fabrication of microfluidic devices using PMMA.

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Section: Introductionmentioning

confidence: 99%

Rapid Fabrication of Poly(methyl methacrylate) Devices for Lab-on-a-Chip Applications Using Acetic Acid and UV Treatment

et al. 2020

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“…The copyright holder for this preprint this version posted December 29, 2020. ; https://doi.org/10.1101/2020.12.28.424535 doi: bioRxiv preprint devices, and this process might disrupt the microfluidic structure (Ng et al, 2016;Shah et al, 2006) as well as cause leakage from the solvents used, thus causing cellular damage (Wan et al, 2017). PMMA also has issues with generating clacking after alcohol treatments (ethanol or isopropanol) for sterilization prior to use in cell culture.…”

Section: Discussionmentioning

confidence: 99%

In vitrononalcoholic fatty liver disease model with cyclo-olefin-polymer-based microphysiological systems

Wen

Yamanaka

Terada

et al. 2020

Preprint

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Nonalcoholic fatty liver disease (NAFLD) is one of the common chronic liver conditions, whose treatment involves curing patients without liver transplantation. Understanding the mechanism of NAFLD initiation and progression would enable development of new diagnostic tools and drugs; however, until now, the underlying mechanisms of this condition remain largely unknown owing to the lack of experimental settings that can simplify the complicated NAFLD process in vitro. Microphysiological systems (MPSs) have long been used to recapture human pathophysiological conditions in vitro for applications in drug discovery. However, polydimethylsiloxane (PDMS) has been used in most of these MPSs as the structural material; it absorbs hydrophobic molecules, such as free fatty acids (FFAs), which are the key components that initiate NAFLD. Therefore, the current PDMS-based MPSs cannot be directly applied to in vitro NALFD modeling. In this work, we present an in vitro NAFLD model with an MPS made of cyclo-olefin polymer (COP), namely COP-MPS, to prevent absorption of FFAs. We demonstrated induction of the NAFLD-like phenotype in HepaRG hepatocyte-like cells cultured in the COP-MPS by introducing FFAs. The FFAs induced lipid accumulation in the HepaRG cells, resulting in inactivation of the apoptotic cells. We believe that the proposed COP-MPS can contribute toward investigations of NAFLD mechanisms and identification of new drugs to prevent the progression of liver disease and avoid liver transplantation.

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“…Ng et al proposed controlling the process parameter by using a thermally activated solvent bonding process. The bonding is based on the temperature-dependent solubility of polymer in a liquid that is not a solvent at room temperature, but which after thermal activation becomes transformed into a solvent of the polymer, creating a chain interdiffusion at the bonding interface [ 58 ]. However, in most cases the effective solubility of solvents for bonding different thermoplastics is obtained by mixing different solvents.…”

Section: Direct Bondingmentioning

confidence: 99%

Recent Advances in Thermoplastic Microfluidic Bonding

Giri

Tsao

2022

Micromachines

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Microfluidics is a multidisciplinary technology with applications in various fields, such as biomedical, energy, chemicals and environment. Thermoplastic is one of the most prominent materials for polymer microfluidics. Properties such as good mechanical rigidity, organic solvent resistivity, acid/base resistivity, and low water absorbance make thermoplastics suitable for various microfluidic applications. However, bonding of thermoplastics has always been challenging because of a wide range of bonding methods and requirements. This review paper summarizes the current bonding processes being practiced for the fabrication of thermoplastic microfluidic devices, and provides a comparison between the different bonding strategies to assist researchers in finding appropriate bonding methods for microfluidic device assembly.

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Low Distortion Solvent Bonding of Microfluidic Chips

Cited by 27 publications

References 8 publications

Rapid Fabrication of Poly(methyl methacrylate) Devices for Lab-on-a-Chip Applications Using Acetic Acid and UV Treatment

Rapid Fabrication of Poly(methyl methacrylate) Devices for Lab-on-a-Chip Applications Using Acetic Acid and UV Treatment

In vitrononalcoholic fatty liver disease model with cyclo-olefin-polymer-based microphysiological systems

Recent Advances in Thermoplastic Microfluidic Bonding

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