Low dose effect of bisphosphonates on hMSCs osteogenic response to titanium surface in vitro

Abstract

Since the 1980s, titanium (Ti) implants have been routinely used to replace missing teeth. This success is mainly due to the good biocompatibility of Ti and the phenomenon of osseointegration, with very early events at implant placement being important in determining good osseointegration. However, enhancing implant performance with coatings such as hydroxyapatite (HA) and calcium phosphate has proved largely unsuccessful. Human mesenchymal stem cells (hMSCs) are the first osteogenic cells to colonise implant surfaces and offer a target for enhancing osseointegration. We previously reported that small doses of bisphosphonate (BP) may play an integral role in enhancing hMSC proliferation and osteogenic differentiation. The aim of this study is to investigate whether small doses of bisphosphonates enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces, to enhance bone osseointegration and to accelerate wound healing around the implant surface. Our data suggests that treating cells with small doses of BP (100 nM & 10 nM) induces significant hMSC stimulation of osteogenic markers including calcium, collagen type I and ALP compared to control group on titanium surfaces (P < 0.05). In addition, cell proliferation and migration were significantly enhanced on titanium surfaces (P < 0.05).

The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

“…The osteoconductivity of P‐34, particularly the increased in‐vitro mineralization in SaOS‐2 cells with P‐34 treatment, was probably due to the PVPA‐ co ‐AA chemical structure, which mimics the action of bisphosphonates, due to a similar chemical structure and the ability of the PO 3 H 2 group to chelate Ca 2+ ions, essentially forming a “bone hook,” with the potential to enhance bone mineralization. Furthermore, bisphosphonates exert stimulatory effects on MSCs proliferation and osteogenic differentiation . As PVPA‐co‐AA has the chemical structure which resembles bisphosphonates, the polymer may mimic the action of bisphosphonate on hBM‐MSCs through as yet an unknown mechanism.…”

Bisphosphonate-based surface biofunctionalization improves titanium biocompatibility

“…Even in small doses of 10 nM, bisphosphonates are able to enhance proliferation and osteogenic differentiation of hMSCs on Ti surfaces. It is currently in the literature that bisphosphonates induce an elevation of ALP activity in drug-treated cells, which produces more calcium when compared to control cells [26,27]. This performance makes bisphosphonates the most employed drugs for the treatment of osteoporosis, and other diseases characterized by abnormally high bone resorption [28,29].…”

Análise Do Percentual De Colágeno Na Dentina Humana Entre Diferentes Etnias, Gêneros E Idades

“…There are countless tests that evaluate cell viability, however, some stand out for being widely used. Among these testes, MTT (Aranha, 2014;Abreu, 2008;Gau, Lai, Leung, 2012;Junior, 2012;Wu et al, 2017;Baili et al, 2016;Galeotti et al, 2013;Hao et al, 2017;Kandiah et al, 2017;Karahalil et al, 2014;Preedy, Perni, Prokopovich, 2017;Silva, 2011;Subramani et al, 2016), MTS (Silva, 2011;Chellini et al, 2017, Gosau et al, 2016Lee et al, 2017), XTT (Aranha, 2014;Coelho, 2013, Fotakis e Timbrell, 2006, Neutral Red (Abreu, 2008;Azevedo, Cruz, Pinto, 2006;Pereira, 2008;Rogero et al, 2003), Alamar Blue (Aranha, 2014;Gao, Lai, Leung, 2012;Gosau et al, 2016;Alqhtani et al, 2017), and the synthesis of nitric oxide (Felgueiras, 2011;Parida et al, 2017;Silva, 2017;Yarlagadda et al, 2017;Zhang, Li, Yang, 2015) can be mentioned.…”