Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Shi, Jun; Wei, Pin‐Kang

doi:10.3892/ol.2015.3641

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…Interestingly, the comparative gene-specific sgRNA enrichment analysis further highlights the global depletion of sgRNA in vivo, but it also shows that sgRNAs targeting certain genes are depleted more profoundly in vivo (Supplementary Figure 3a-b ). Notably, genes that play a key role in cell adhesion and migration such as IL8 20 , 21 , cadherin coding FZR1 22 , and metastasis-related EGFL6 23 are among the most depleted genes discovered in the in vivo screen (Supplementary Figure 3a ). Overall, CRISPR-mediated gene-based viability scores also identified genes that are consistently depleted in both in vitro and in vivo control samples.…”

Section: Resultsmentioning

confidence: 99%

CRISPR knockout screening identifies combinatorial drug targets in pancreatic cancer and models cellular drug response

Szlachta¹,

Kuscu²,

Tufan³

et al. 2018

Nat Commun

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Predicting the response and identifying additional targets that will improve the efficacy of chemotherapy is a major goal in cancer research. Through large-scale in vivo and in vitro CRISPR knockout screens in pancreatic ductal adenocarcinoma cells, we identified genes whose genetic deletion or pharmacologic inhibition synergistically increase the cytotoxicity of MEK signaling inhibitors. Furthermore, we show that CRISPR viability scores combined with basal gene expression levels could model global cellular responses to the drug treatment. We develop drug response evaluation by in vivo CRISPR screening (DREBIC) method and validated its efficacy using large-scale experimental data from independent experiments. Comparative analyses demonstrate that DREBIC predicts drug response in cancer cells from a wide range of tissues with high accuracy and identifies therapeutic vulnerabilities of cancer-causing mutations to MEK inhibitors in various cancer types.

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Section: Resultsmentioning

confidence: 99%

CRISPR knockout screening identifies combinatorial drug targets in pancreatic cancer and models cellular drug response

Szlachta¹,

Kuscu²,

Tufan³

et al. 2018

Nat Commun

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“…In the present study, we classified the expression level of CXCL8 into three groups: negative, low, and high. As low-dose CXCL8 was reported to induce the migration and invasion of human gastric cancer cell lines [ 45 ], we suspected that even a low expression level of CXCL8 would be associated with tumor progression. Our investigation revealed that a high expression level of CXCL8 by ESCC tissue was significantly associated with DFS, lymph node metastasis, a high expression level of CXCR2, and the invasion of high numbers of CD204-positive macrophages.…”

Section: Discussionmentioning

confidence: 99%

CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells

et al. 2017

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Tumor-associated macrophages (TAMs) are involved in tumor progression and poor prognosis in several malignancies. We previously demonstrated the interaction between high numbers of infiltrating TAMs and poor prognosis in esophageal squamous cell carcinomas (ESCCs). To investigate the significance of TAMs in ESCC, we conducted a cDNA microarray analysis of peripheral blood monocytes (PBMo)-derived macrophages and PBMo-derived macrophages stimulated with conditioned media of TE-series ESCC cell lines (TAM-like PBMo-derived macrophages). C-X-C motif chemokine ligand 8 (CXCL8) was up-regulated in the TAM-like PBMo-derived macrophages. Here we confirmed a high expression level of CXCL8 in TAM-like PBMo-derived macrophages and the expression of CXCR1/2, known as CXCL8 receptors, in TE-series ESCC cell lines. Recombinant human CXCL8 induced the ESCC cell lines’ migration and invasion by the phosphorylation of Akt and Erk1/2. In indirect co-cultures, not only signal pathway inhibitors but also neutralizing antibodies against CXCL8, CXCR1 and CXCR2 suppressed these phenotypes induced by TAM-like PBMo-derived macrophages. Immunohistochemical analysis of 70 resected ESCC samples showed that high expression levels of CXCL8 in ESCC tissues were significantly associated with lymph node metastasis and poor prognosis. These results suggest that CXCL8 up-regulated in the microenvironment may contribute to ESCC progression by promoting cancer cells’ migration and invasion.

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“…Previous studies have reported the impact of IL-8 on the migration and invasion of tumor cells (21). However, those studies neglected that SKOV3 cells themselves can produce IL-8 thus impacting cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, identification of appropriate therapeutic targets is crucial in the latest evolution in the treatment of ovarian cancer. Previous studies have reported the impact of IL-8 on the migration and invasion of tumor cells ( 21 ). However, those studies neglected that SKOV3 cells themselves can produce IL-8 thus impacting cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells

Liu

Yin

et al. 2016

Oncology Letters

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The aim of the study was to determine whether interleukin-8 (IL-8) affects human SKOV3 ovarian cancer cell migration and invasion by targeting silencing of IL-8 expression. Silencing small-interfering RNA (siRNA) targeting IL-8 gene was constructed to infect SKOV3 cells by lentiviral vector. The expression of IL-8 and p-nuclear factor (NF)-κB protein was detected by western blot analysis. The wound scratch and Transwell tests were used to assay the cell migration and invasiveness of SKOV3 cells infected with lentiviral vector targeting IL-8 gene siRNA. The levels of IL-8 protein expressed by SKOV3 cells infected by lentiviral vector targeting IL-8 gene siRNA decreased by 72.3%. IL-8 (50 ng/ml) increased the ability of SKOV3 cells to suppress cell migration (p<0.01). Cisplatin and silencing of IL-8 achieved the ability to inhibit SKOV3 cell invasion (p<0.01), and 100 ng/ml concentration of IL-8 enhanced the ability of SKOV3 invasion (p<0.01). Silencing of IL-8 to a certain extent reduced the expression of p-NF-κB proteins, but it was not statistically significant. In conclusion, silencing of IL-8 may inhibit the migration and invasion of SKOV3 cells, which may be independent of the p-NF-κB protein.

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Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Cited by 7 publications

References 40 publications

CRISPR knockout screening identifies combinatorial drug targets in pancreatic cancer and models cellular drug response

CRISPR knockout screening identifies combinatorial drug targets in pancreatic cancer and models cellular drug response

CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells

Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells

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