Low-Dose Ionizing Radiation Stimulates Transcription and Production of Endothelin by Human Vein Endothelial Cells

Lanza, Valentina; Fadda, Paolo; Iannone, Camilla; Negri, Rodolfo

doi:10.1667/rr0780.1

Cited by 20 publications

(17 citation statements)

References 54 publications

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“…This probably reflects the peculiarity of the response of endothelial cells to irradiation which has been previously described in detail (17). Furthermore most of the experiments performed on melanoma cell lines (experiments 159–164) tend to cluster together independently from irradiation with X-rays or HZE particles.…”

Section: Applicationsupporting

confidence: 54%

“…Low-LET radiation is comprised of X-, β-and γ-rays, while neutrons, high charge ( Z ) and energy ( E ) nuclei (HZE) and α-particles are high-LET radiation. There are qualitative differences between high- and low-LET radiation both in the induction and repair of DNA damage (14,16,17). Although high-LET radiation is widely used in medical applications and knowledge of its biological effects is crucial for human protection during space flights (14), at present, very few studies are available on its impact on gene transcription modulation.…”

Section: Introductionmentioning

confidence: 99%

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Radiation Genes: a database devoted to microarrays screenings revealing transcriptome alterations induced by ionizing radiation in mammalian cells

Chiani¹,

Iannone²,

Negri³

et al. 2009

Database

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The analysis of the great extent of data generated by using DNA microarrays technologies has shown that the transcriptional response to radiation can be considerably different depending on the quality, the dose range and dose rate of radiation, as well as the timing selected for the analysis. At present, it is very difficult to integrate data obtained under several experimental conditions in different biological systems to reach overall conclusions or build regulatory models which may be tested and validated. In fact, most available data is buried in different websites, public or private, in general or local repositories or in files included in published papers; it is often in various formats, which makes a wide comparison even more difficult. The Radiation Genes Database (http://www.caspur.it/RadiationGenes) collects microarrays data from various local and public repositories or from published papers and supplementary materials. The database classifies it in terms of significant variables, such as radiation quality, dose, dose rate and sampling timing, as to provide user-friendly tools to facilitate data integration and comparison.

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Section: Applicationsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Radiation Genes: a database devoted to microarrays screenings revealing transcriptome alterations induced by ionizing radiation in mammalian cells

Chiani¹,

Iannone²,

Negri³

et al. 2009

Database

Self Cite

View full text Add to dashboard Cite

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“…Indeed, in vitro and in vivo studies have shown that various chemical and physical stresses modify ET-1 gene expression and lead to its secretion by EC. 51 The study by Lanza et al 50 showed that irradiated HUVEC cells increased production of ET-1, but the ET-1 then accumulated within the cytoplasm of the endothelial cell.…”

Section: Radiation Effectsmentioning

confidence: 99%

“…Irradiation significantly increases the response to an inflammatory stimulus leading to the upregulation of ICAM-1. 49 Lanza et al 50 studied the production of ET-1 by irradiated cultured HUVEC. ET-1, highly expressed by AVM EC, 34 is a potent vasoconstrictor peptide that has a role in the proliferation of smooth muscle cells.…”

Section: Radiation Effectsmentioning

confidence: 99%

Endothelial cells in the context of brain arteriovenous malformations

Sammons

Davidson

et al. 2011

Journal of Clinical Neuroscience

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“…Immediate and early responses to radiation have been observed with kinetics of less than 30 min. Such rapid responses include conversion of sphingomyelin to ceramide and the induction of endothelin transcripts (44,45). The differences in kinetics could arise from the endothelial cell type as discussed above or from a difference in the extracellular matrix (ECM).…”

Section: Discussionmentioning

confidence: 99%

Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation

Young

Smilenov

2011

Radiation Research

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The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported.

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Low-Dose Ionizing Radiation Stimulates Transcription and Production of Endothelin by Human Vein Endothelial Cells

Cited by 20 publications

References 54 publications

Radiation Genes: a database devoted to microarrays screenings revealing transcriptome alterations induced by ionizing radiation in mammalian cells

Radiation Genes: a database devoted to microarrays screenings revealing transcriptome alterations induced by ionizing radiation in mammalian cells

Endothelial cells in the context of brain arteriovenous malformations

Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation

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