Low dose of protein A pretreatment can alleviate the inflammatory reaction and the bio-safety was evaluated in vivo

Wang, Dong; Liu, Yang; Zhao, Ye; Zhou, Junlin

doi:10.1016/j.jcma.2016.01.010

Cited by 6 publications

(7 citation statements)

References 16 publications

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“…The rats were gently handled and removed from their cages. The probe was inserted 1 cm into the rectum and the temperature was measured at 09:00 a.m. 20 Blood was harvested on post-irrigation days 1, 3, 5, 7, 14 and 28. Blood was centrifuged at 3000 rpm/min for 25 min, to separate the serum.…”

Section: Evaluation Indicatorsmentioning

confidence: 99%

“…The pathway of interleukin‐6 (IL‐6), interleukin‐10 (IL‐10), bone morphogenetic protein (BMP‐2), vascular endothelial growth factor (VEGF) and transforming growth factor (TGF‐β1) during wound healing process were complex. Farahpour et al 20 . evaluated the effects of salvia officinalis essential oil (SOO) on an infected wound mice model.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19] The pathway of interleukin-6 (IL-6), interleukin-10 (IL-10), bone morphogenetic protein (BMP-2), vascular endothelial growth factor (VEGF) and transforming growth factor (TGF-β1) during wound healing process were complex. Farahpour et al 20 evaluated the effects of salvia officinalis essential oil (SOO) on an infected wound mice model. They found that SOO increased the tissue total antioxidant capacity level and reduced the malondialdehyde, interleukin-1β and tumor necrosis factor-α levels.…”

Section: Introductionmentioning

confidence: 99%

“…Farahpour et al . 20 evaluated the effects of salvia officinalis essential oil (SOO) on an infected wound mice model. They found that SOO increased the tissue total antioxidant capacity level and reduced the malondialdehyde, interleukin‐1β and tumor necrosis factor‐α levels.…”

Section: Introductionmentioning

confidence: 99%

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The Toxicity and Antibacterial Effects of Povidone‐Iodine Irrigation in Fracture Surgery

Wang

Huang

et al. 2022

Orthopaedic Surgery

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Objective Surgical site infection is a common complication of surgery, especially in orthopedics. Povidone‐Iodine (PI) is one of the oldest and most commonly used disinfectants in surgery. However, the toxicity and antimicrobial effect of PI have not been discussed. In addition, no study has explored the optimum PI concentration for sterilization and tissue healing. This study explores the germicidal efficacy of different concentrations PI, in addition, the toxicity and antibacterial effects of PI irrigation in fracture surgery are also discussed. Methods Methicillin‐resistant Staphylococcus aureus and Pseudomonas aeruginosa (P. aeruginosa) were used to evaluate the germicidal efficacy of PI in vitro and in vivo. In vitro, the effects of PI on bacterial growth were analyzed. 2.5%, 1.25%, 0.5%, 0.25%, 0.05%, 0.025%, 0.005%, 0.0025% and 0% PI was added into the bacterial suspension, besides, the bacterial algebra and growth rate were tested. Meanwhile, the fluorescence intensity of viable bacteria was also tested to evaluate the effects of PI on bacterial survival. In vivo, first, femoral fracture with wound infection rat models were established. Second, thyroid gland sections, blood thyroxine, urinary iodine, wound local skin, muscle and bone tissue sections, serum creatinine and alanine aminotransferase, serum and bone local tissue interleukin‐6 (IL‐6), interleukin‐10 (IL‐10), bone morphogenetic protein (BMP‐2), vascular endothelial growth factor (VEGF) and transforming growth factor (TGF‐β1) were detected in rat femoral shaft fracture model with 5%, 2.5%, 0.5%, 0.05%, and 0% PI irrigation. Third, tissue bacteria culture was tested in rat femoral fracture with wound infection model with different concentrations PI irrigation. Results In vitro, 2.5%, 1.25%, 0.5% PI inhibited the growth of bacteria. 1.25%, 0.5% PI killed all the bacteria, while 0.25%, 0.05% PI had not killed bacteria after about 10 min. The iodine absorption of 5%, 2.5%, 0.5% PI irrigation did not cause thyroid injury. The 5%, 2.5%, 0.5% PI irrigation did not make serum creatinine and alanine aminotransferase abnormal and can remove bacteria from wounds. The 0.5%, 2.5% PI irrigation can promote tissue healing and increase BMP‐2, VEGF, TGF‐β1, IL‐10, in addition, decrease IL‐6. 5% PI irrigation would inhibit tissue healing, and increase IL‐6, decrease BMP‐2, VEGF, TGF‐β1, IL‐10. Conclusions Povidone‐Iodine was a widely used disinfectant and 2.5%, 1.25% and 0.5% PI could effectively kill bacteria. Five percent and lower concentration PI irrigation was safe and could not cause thyroid, kidney and liver damage. The 0.5% PI irrigation was beneficial for tissue healing but 5% PI irrigation was the opposite.

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Section: Evaluation Indicatorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Toxicity and Antibacterial Effects of Povidone‐Iodine Irrigation in Fracture Surgery

Wang

Huang

et al. 2022

Orthopaedic Surgery

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“…The movement of the hip and knee, the contraposition and alignment of the fracture, as well as the xation of the fracture, were examined. The method of causing bacterial infection was described as the previously study [10]. Brie y, the bacterial suspension was administered to the rats by gradual dripping onto the surface of the muscle and embrocation with a sterile bacterial inoculation needle.…”

Section: Animalsmentioning

confidence: 99%

Gentamicin Sponge for Anti-infection, a Controversial Old Topic and Preliminary Exploration of the Solution, in Vitro and in Vivo Experiments

Wang

Zhou

2021

Preprint

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Purpose: Our study was to construct drug-loading and drug-release quantitative equation of gentamicin sponge, in addition, obtain the wound infection prevented and treated scheme of gentamicin sponge. Methods: Sterile sponge was cut into 1×1×0.5cm size and immersed into 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml or 0mg/ml gentamicin solution for 12h, 24h, 48h, 96h or 120h to evaluate gentamicin-loading of sponge. Sponge was immersed in gentamicin solution of different concentrations for 48h and then air-dried. Air-dried sponge was immersed into 10ml 0.9% physiological saline to evaluated gentamicin-release. Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa (P. aeruginosa) were used to explore infection prevented scheme of gentamicin sponge. Besides, femur fractured with wound infection rat model was used to discuss the infection treated scheme.Results: The equation of gentamicin-loading of sponge was: z=(0.03718±0.01672)x+(-4.578e-4±0.06253)y+(-2.50935e-4±1.47521e-4)x2+(0.00303±0.00149)y2+(0.00408±3.52827e-4)xy (R2 was 0.97) and drug-release equation was z=(4.37205±1.18048)x+(-7.05921±3.09628)y+(-0.04596±0.01287)x2+(0.3309±0.07912)y2+(0.31559±0.02754)xy (R2 was 0.95). The antibacterial zone sizes of sponges immersed in 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml gentamicin solution were larger than 0mg/ml air-dried sponge, and the difference had statistically significant (p<0.01). Rats of groups of 40mg/ml air-dried sponge, 16mg/ml air-dried sponge, 8mg/ml air-dried sponge had no wound suppuration in both MSSA and P. aeruginosa infection rat model.Conclusions: The quantified equation of gentamicin-loading and release of sponge was with high accuracy. 1.6 mg/ml air-dried sponge and 0.8mg/ml air-dried sponge were enough to prevent wound infection. Besides, if wound were confirmed to be sensitive bacteria infected, we recommended to use 40mg/ml air-dried sponge, 16mg/ml air-dried sponge or 8mg/ml air-dried sponge to treat.

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Binimetinib ameliorates the severity of septic cardiomyopathy by downregulating inflammatory factors

Zheng

Wang

et al. 2022

International Immunopharmacology

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Low dose of protein A pretreatment can alleviate the inflammatory reaction and the bio-safety was evaluated in vivo

Abstract: SPA pretreatment can effectively decrease the infected severity of a wound infected by various bacteria in a BALB/c mouse model. The best pretreatment dose is 1 mg/kg, and this dose does not damage organ function in rats up to a point.

Cited by 6 publications

References 16 publications

The Toxicity and Antibacterial Effects of Povidone‐Iodine Irrigation in Fracture Surgery

The Toxicity and Antibacterial Effects of Povidone‐Iodine Irrigation in Fracture Surgery

Gentamicin Sponge for Anti-infection, a Controversial Old Topic and Preliminary Exploration of the Solution, in Vitro and in Vivo Experiments

Binimetinib ameliorates the severity of septic cardiomyopathy by downregulating inflammatory factors

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