2000
DOI: 10.1093/toxsci/55.1.97
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Low-Dose Whole-Body Irradiation (LD-WBI) Changes Protein Expression of Mouse Thymocytes: Effect of a LD-WBI-Enhanced Protein RIP10 on Cell Proliferation and Spontaneous or Radiation-Induced Thymocyte Apoptosis

Abstract: Low-dose radiation (LDR) can potentiate cellular metabolic activities or immune functions in vivo (hormesis), and can render cells resistant to DNA or chromosome damage caused by subsequent high-dose radiation (adaptive response). Protein synthesis was required for these cellular responses to LDR. In the present study, the early expression of proteins by thymocytes in response to low-dose whole-body irradiation (LD-WBI) was investigated. The expression of novel and previously existing proteins was found in the… Show more

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Cited by 49 publications
(28 citation statements)
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“…8,9 Although the dose level is far from lethal, it might be high enough to cause biological effects that could interfere with the investigation. Several low-dose radiation effects have been reported [10][11][12][13][14][15][16][17][18][19] including effects on tumor growth and hematopoiesis. As microCT technology evolves rapidly, the limiting factor in high quality images will be most likely the x-ray dose administered.…”
Section: Introductionmentioning
confidence: 99%
“…8,9 Although the dose level is far from lethal, it might be high enough to cause biological effects that could interfere with the investigation. Several low-dose radiation effects have been reported [10][11][12][13][14][15][16][17][18][19] including effects on tumor growth and hematopoiesis. As microCT technology evolves rapidly, the limiting factor in high quality images will be most likely the x-ray dose administered.…”
Section: Introductionmentioning
confidence: 99%
“…Flow cytometry analysis of apoptotic cell death in cultured thymocytes and splenocytes has been extensively used in our previous studies. [11][12][13] In addition, apoptotic cell death has also been confirmed by another standard method, DNA fragmentation (Fig. 2).…”
Section: Discussionmentioning
confidence: 70%
“…However, little is known about the effects of bFGF on organs involved in immune regulation such 655-659 (2006) mocyte suspensions were prepared as described in previous studies. [11][12][13] Both types of cells were incubated in 24-well plates at 5 × 10 6 cells/ml in the presence or absence of bFGF 1.0 or 2.0 µg/ml. Since it is well known that radiation-induced cell death has two phases, the immediate effect of predominantly necrotic cell death and the delayed mainly apoptotic cell death, the antiapoptotic effects of bFGF were observed by adding it to the cultured cells when they were collected from animals 14 hr after irradiation.…”
Section: )mentioning
confidence: 99%
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“…Ionizing radiation has been shown to modulate gene and protein expression as well as cellular signal transduction [19][20][21]. Next, we investigated the effect of low-dose irradiation on the tyrosine-phosphorylation of cytosolic and nuclear proteins.…”
Section: Alteration In the Level Of Tyrosinephosphorylated Proteins Imentioning
confidence: 99%