Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response

Sun, Bin; Luan, Chunye; Guo, Lisha; Zhang, Bing; Liu, Yufang

doi:10.1590/1414-431x20209501

Cited by 19 publications

(15 citation statements)

References 38 publications

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“…The results showed that serum levels of LDH, AST, ALT, CK-MB, and HDBH in NP treatment groups (0.4 mg/kg and 40 mg/kg) increased significantly, indicating that the myocardial tissue was damaged. This result is consistent with the findings of Wang et al [36], suggesting that the heart may be the target organ of NP poisoning.…”

Section: Discussionsupporting

confidence: 93%

Effects of Long-term Nonylphenol Exposure on Myocardial Fibrosis and Cardiac Function in Rats

Liu

et al. 2021

Preprint

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Background: Myocardial fibrosis is a critical pathological basis for the poor prognosis of cardiovascular diseases. Studies have found that myocardial fibrosis is closely associated with exposure to environmental estrogens such as nonylphenol (NP), as a representative of environmental estrogens. The aim of this study was to examine the effects of NP chronic exposure on myocardial fibrosis as well as cardiac structure and function. Forty Sprague Dawley rats were randomly divided into four groups (n = 10): control group (C), low NP dose (0.4 mg/kg, L), medium NP dose (4 mg/kg, M), and high NP dose (40 mg/kg, H) groups. The NP dose groups were gavaged with NP for 180 days. Results: The NP level in the heart of the NP groups was significantly higher than those in the control group (F = 43.658, P < 0.001). Serum aspartate aminotransferase (AST), creatine kinase (CK), creatine kinase isozyme (CK-MB), lactate dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (α-HBDH) significantly increased in the NP groups compared with the control group (). Histopathological examination of the heart biopsy illustrates that in the medium and high NP groups, the fibrous connective tissue had a disordered and loose gridding shape, muscle fibers had fractured, and muscle fibers were loose with a widened gap. Extensive inflammatory cell infiltration and fibroblast proliferation in the myocardial interstitium were also found. With increasing NP dose, the degree of muscle fiber loosing and disorder became more significant in the NP treatment groups, and the collagen volume fraction (CVF) was higher than that in the control group (P < 0.01). Compared with the control group, the expression of collagen I and collagen III increased significantly in the medium and high NP groups (P < 0.05). The values of the systolic thickness of the left ventricular anterior wall (LVAWs), the diastolic thickness of the left ventricular posterior wall (LVPWd), the systolic thickness of the left ventricular posterior wall (LVPWs), and the left ventricular anterior wall (LVAWd) in the NP groups are were slightly lower than those in of the control group. The values of left ventricular end systolic dimensions (LVIDs) in the NP groups increased compared with the control group. Conclusions: Long-term NP exposure could lead to fibrosis in the rat myocardium, which is characterized by increased expressions of myocardial collagen I and collagen III, as well as elevated cardiac enzymes. In addition, the cardiac structure was affected and changes were observed in the thinner ventricular wall and as an enlarged ventricular cavity.

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Section: Discussionsupporting

confidence: 93%

Effects of Long-term Nonylphenol Exposure on Myocardial Fibrosis and Cardiac Function in Rats

Liu

et al. 2021

Preprint

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“…Sun et al recognized a positive correlation between miR-328 serum level and sepsis in human patients [ 68 ]. Moreover, miR-328 showed to be correlated with cardiac dysfunction in the CLP rat model, while the injection of its antagomiR seemed to reduce inflammation and ameliorate the impairment.…”

Section: Resultsmentioning

confidence: 99%

“…Ge et al 2018 [52] MiR-214-3p Sang et al 2020 [53] MiR-218a-5p ↑ -Zhang et al [69] MiR-219a-1-3p ↑ -Zhang et al [69] MiR-223-5p ↓ Sema3A Wang et al 2014 [32] MiR-233-3p ↑ -Zhang et al [69] MiR-233-5p ↑ -Zhang et al [69] MiR-322-5p ↓ -Zhang et al [69] MiR-328 ↑ its inhibition is protective -Sun et al 2020 [68] MiR-330-5p ↑ -Zhang et al [69] ↓ its up-regulation is protective TRAF6 Xing et al 2020 [74] MiR-339-3p ↑ -Zhang et al [69] MiR-340-3p ↑ -Zhang et al [69] MiR-362-5p ↓ -Zhang et al [69] MiR-369-5p ↑ -Zhang et al [69] MiR-378a-5p ↓ -Zhang et al [69] MiR-379-5p ↑ -Zhang et al [69] MiR-380-3p ↑ -Zhang et al [69] MiR-409a-3p ↑ -Zhang et al [69] MiR-425-3p ↓ -Zhang et al [69] MiR-434-5p ↑ -Zhang et al [69] MiR-466b-5p ↑ -Zhang et al [69] MiR-490-5p ↑ -Zhang et al [69] MiR indicates microRNA; HSPA4, the heat shock protein previously known as hsp70; SORBS2, sorbin and SH3 domain containing 2; TGIF1, 5 TG3 -interacting factor 1; PTEN, phosphatase and tensin homolog; TRAF6, tumor necrosis factor receptor-associated factor 6; IκκB, inhibitor of nuclear factor kappa-B kinase subunit beta; XIAP, X-chromosome-linked inhibitor of apoptosis; Nrf2, nuclear factor (erythroid-derived 2)-like 2; SIRT1, sirtuin 1; TRL4, toll-like receptor 4; HMGA2, high mobility group at-hook 2; STX2, syntaxin-2; IRAK1, interleukin-1 receptor-associated kinase 1; ErbB4, erb-B2 receptor tyrosine kinase 4; Akt2, serine/threonine kinase 2; Pea15a, phosphoprotein enriched in astrocytes; Arrb2, β-arrestin 2; SOCS2, suppressor of cytokine signaling 2; Sema3A, semaphorin3A; AQP1, aquaporin 1.…”

Section: Ptenmentioning

confidence: 99%

MicroRNAs and Sepsis-Induced Cardiac Dysfunction: A Systematic Review

Manetti

Maiese

Paolo

et al. 2020

IJMS

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Sepsis is a severe condition characterized by systemic inflammation. One of the most involved organs in sepsis is the heart. On the other hand, heart failure and dysfunction are some of the most leading causes of death in septic patients. miRNAs are short single-strand non-coding ribonucleic acids involved in the regulation of gene expression on a post-transcriptional phase, which means they are a part of the epigenetic process. Recently, researchers have found that miRNA expression in tissues and blood differs depending on different conditions. Because of this property, their use as serum sepsis biomarkers has also been explored. A narrative review is carried out to gather and summarize what is known about miRNAs’ influence on cardiac dysfunction during sepsis. When reviewing the literature, we found at least 77 miRNAs involved in cardiac inflammation and dysfunction during sepsis. In the future, miRNAs may be used as early sepsis-induced cardiac dysfunction biomarkers or as new drug targets. This could help clinicians to early detect, prevent, and treat cardiac damage. The potential role of miRNAs as new diagnostic tools and therapeutic strategies worth deepening the complex network between non-coding RNA and biological pathways. Additional studies are needed to further investigate their role in sepsis-induced myocardium injury.

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“…However, the expression levels of novel miRNA were lower than those of known miRNAs. Low miRNA expression plays a certain role in the regulation of cells biological responses [ 29 ]. Conversely, high miRNA expression plays a key roles in the regulation of targeted molecules in tissues and cells, indicating that miRNAs are highly involved in intracellular molecular regulation [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Multi–Omics Analysis of Key microRNA–mRNA Metabolic Regulatory Networks in Skeletal Muscle of Obese Rabbits

Wang

Elzo

et al. 2021

IJMS

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microRNAs (miRNAs), small non-coding RNA with a length of about 22 nucleotides, are involved in the energy metabolism of skeletal muscle cells. However, their molecular mechanism of metabolism in rabbit skeletal muscle is still unclear. In this study, 16 rabbits, 8 in the control group (CON–G) and 8 in the experimental group (HFD–G), were chosen to construct an obese model induced by a high–fat diet fed from 35 to 70 days of age. Subsequently, 54 differentially expressed miRNAs, 248 differentially expressed mRNAs, and 108 differentially expressed proteins related to the metabolism of skeletal muscle were detected and analyzed with three sequencing techniques (small RNA sequencing, transcriptome sequencing, and tandem mass tab (TMT) protein technology). It was found that 12 miRNAs and 12 core genes (e.g., CRYL1, VDAC3 and APIP) were significantly different in skeletal muscle from rabbits in the two groups. The network analysis showed that seven miRNA-mRNA pairs were involved in metabolism. Importantly, two miRNAs (miR-92a-3p and miR-30a/c/d-5p) regulated three transcription factors (MYBL2, STAT1 and IKZF1) that may be essential for lipid metabolism. These results enhance our understanding of molecular mechanisms associated with rabbit skeletal muscle metabolism and provide a basis for future studies in the metabolic diseases of human obesity.

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Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response

Cited by 19 publications

References 38 publications

Effects of Long-term Nonylphenol Exposure on Myocardial Fibrosis and Cardiac Function in Rats

Effects of Long-term Nonylphenol Exposure on Myocardial Fibrosis and Cardiac Function in Rats

MicroRNAs and Sepsis-Induced Cardiac Dysfunction: A Systematic Review

Multi–Omics Analysis of Key microRNA–mRNA Metabolic Regulatory Networks in Skeletal Muscle of Obese Rabbits

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