Low fouling strategies for electrochemical biosensors targeting disease biomarkers

Liu, Nianzu; Xu, Zhenying; Morrin, Aoife; Liu, Xiang

doi:10.1039/c8ay02674b

Cited by 92 publications

(60 citation statements)

References 75 publications

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“…On the PEG hydrogel sample, aggregates of platelets were observed throughout the surface of the hydrogel, although their morphology was generally round, indicating that the platelets were not activated. Similarly, PHEMA hydrogel surface exhibited aggregates of round platelets present across the surface 56 .…”

Section: Protein Adsorption and Platelet-resistant Hydrogelsmentioning

confidence: 95%

“…The use of platelet counts allowed for inputs that can link polymer behaviors to anti-biofouling performance, in contrast to protein adsorption assays that may not yield predictive models 41 . The long-withstanding hypothesis regarding the anti-fouling mechanism of the "gold standard" polymers is the presence of a tight hydration layer between the material surface and water that forms a physical and energetic barrier to protein adsorption, determined often by the physicochemical properties of the materials and the surface packing 56 . These properties include surface wettability and hydrophilicity, electrical neutrality, H-bonding, and prevalence of highly hydrated chemical groups.…”

Section: Identifying Key Features Of Monomers For Anti-biofouling Promentioning

confidence: 99%

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Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

Chan

Chien

Axpe

et al. 2020

Preprint

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Biofouling on the surface of implanted medical devices severely hinders their functionality and drastically shortens their lifetime. Currently, poly(ethylene glycol) and zwitterionic polymers are considered “gold standards” for device coatings to reduce biofouling. To discover novel antibiofouling materials, we created a combinatorial library of polyacrylamide-based copolymer hydrogels and screened their ability to prevent fouling from serum and platelet-rich plasma in high-throughput. We found certain non-intuitive copolymer compositions exhibit superior antibiofouling properties than current gold-standard materials and employed machine learning to identify key molecular features underpinning their performance. For validation, we coated the surfaces of electrochemical biosensors with our hydrogels and evaluated their anti-biofouling performance in vitro and in rodent models. Our copolymer hydrogels preserved device function over multiple days and enabled continuous measurements of a small-molecule drug in vivo. The novel methodology we describe enables the discovery of anti-biofouling materials that can extend the lifetime of implanted devices.

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Section: Protein Adsorption and Platelet-resistant Hydrogelsmentioning

confidence: 95%

Section: Identifying Key Features Of Monomers For Anti-biofouling Promentioning

confidence: 99%

Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

Chan

Chien

Axpe

et al. 2020

Preprint

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“…Small published by Wiley-VCH GmbH in spurious signals and eventual loss of signal when the surface is covered by a so-called protein corona. [157,158] In point-of-care biosensors, biofouling has even led to patient infection. [159,160] Because the materials used vary between different sensor designs, a set of universal antifouling strategies is impossible to identify.…”

Section: (14 Of 19)mentioning

confidence: 99%

Plasmonic Assemblies for Real‐Time Single‐Molecule Biosensing

Armstrong

Horáček

Zijlstra

2020

Small

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and bound states in the continuum, [27,28] and have been used in applications like biosensing [29] and color printing. [30] Plasmonic assemblies have proven particularly powerful in the field of biosensing. The presence of a biomarker results in, e.g., the (dis-)assembly of solution-phase plasmonic particles, or to changes in the particle-spacing in an assembly. Both result in strong changes in the far-field optical response of the plasmonic assemblies that is most often monitored using spectroscopy in a UV-vis spectrometer. In solution-based sensors, clustering of metal nanoparticles can be induced by target molecules, shifting the optical extinction spectrum of the nanoparticle solution (Figure 1d). [31] Such clustering assays based on particle (dis-) assembly have resulted in colorimetric assays for a range of analytes including proteins, DNA/RNA sequences, heavy ions, and toxic compounds. [31,32] Single-molecule biosensors, on the other hand, detect analytes by probing the optical response of a single nanoparticle. An important advantage of single-molecule sensing is the ability to construct histograms of sensor properties at the single-molecule level, e.g., the induced plasmon shift and statistical parameters like the waiting-time between events. Single-molecule biosensing using plasmonic sensors has been demonstrated in 2012 by Figure 1. Reported applications of nanoparticle assemblies. a) Left: Calculated SERS enhancement factors for a sphere dimer with a 2 nm gap spacing. Right: A Raman spectra of methylene blue dispersed on a monolayer (black) and bilayer (red) of gold nanoparticles. Adapted with permission. [11] Copyright 2019, American Chemical Society. (https://pubs.acs.org/doi/10.1021/acsnano.9b04224) Further permissions related to this material should be directed to the ACS. b) Non-linear emission spectrum of noncentrosymmetric patterned gold nanorods. The third-harmonic generation (THG) peak is in green and the second-harmonic generation (SHG) is in orange. Top inset-scheme of the assembly's THG (green) and SHG (orange) stemming from the red excitation wavelength (ω). Bottom inset-electron microscopy image of the nanoassembly (scale bar 200 nm). Adapted with permission. [16] Copyright 2019, American Chemical Society. c) Examples of metamaterials comprised of plasmonic assemblies (scale bars are 500 nm). Reproduced with permission. [25] Copyright 2014, Springer Nature. d) Left: A cluster assay biosensor in which nanoparticle assembly is induced by a target molecule. Right: An example UV-vis spectrum of the gold nanoparticle monomers (red) and subsequent assemblies upon addition of target molecules (blue).

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“…This can be related to the fouling of the electrode ascribed to the nonspecific protein adsorption and cell adhesion. In analytical electrochemistry, cell biofouling is a well-known phenomenon that has a harmful effect on the functioning of the electrochemical sensing interface when exposed to a biofluid (Liu, et al, 2019). Different open-circuit values were obtained for each electrode mentioned above.…”

Section: Electrochemical Cleaning Process and Surface Conditions Of Gmentioning

confidence: 99%

Limpieza de electrodos y reproducibilidad de medidas de impedancia eléctrica en células HeLa en solución acuosa

Pinto

Pinzón

Meléndez

et al. 2020

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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La espectroscopia de impedancia eléctrica (EIS) se emplea en el estudio de las propiedades eléctricas de células en suspensión. Para medir las propiedades eléctricas de materiales se utilizan dos (sistema de electrodo bipolar), tres (tripolares) y cuatro (tetrapolares) electrodos, siendo las medidas tetrapolares la mejor opción para minimizar los efectos de polarización de electrodo. Cuando se repiten las medidas de EIS en una misma muestra, se espera obtener espectros similares, sin embargo, en algunas situaciones se encuentran diferencias significativas asociadas con la baja reproducibilidad y repetibilidad. Aquí se utilizaron células HeLa dispersas en solución acuosa para evaluar también el efecto de la limpieza en el funcionamiento de los electrodos de oro. La limpieza de las superficies de los electrodos de oro se hizo por voltamperometría cíclica. Se encontró que la reproducibilidad y la repetibilidad de las medidas de impedancia aumentaba al limpiar los electrodos. Estos resultados evidenciaron que la limpieza de la superficie de los electrodos minimizó las contribuciones de la polarización de los electrodos en las mediciones. Además, se propone un modelo de circuito para describir el efecto de las superficies sin limpiar en las medidas eléctricas. Dicho modelo confirma que el efecto observado no se debió a una impedancia de polarización convencional. Más bien se trató de una polarización activa (como un potencial), la cual se presenta a bajas frecuencias y es independiente de la frecuencia de la señal de excitación.

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Low fouling strategies for electrochemical biosensors targeting disease biomarkers

Abstract: This minireview summarizes recent trends in the development of low fouling electrochemical biosensors based on different antifouling materials.

Cited by 92 publications

References 75 publications

Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

Plasmonic Assemblies for Real‐Time Single‐Molecule Biosensing

Limpieza de electrodos y reproducibilidad de medidas de impedancia eléctrica en células HeLa en solución acuosa

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