Low-Intensity Pulsed Ultrasound Enhances BMP-7-Induced Osteogenic Differentiation of Human Fracture Hematoma-Derived Progenitor Cells In Vitro

Lee, Sang Yang; Koh, Akihiro; Niikura, Takahiro; Oe, Keisuke; Koga, Takaaki; Dogaki, Yoshihiro; Kurosaka, Masahiro

doi:10.1097/bot.0b013e3182519492

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…The Institutional Review Board of Kobe University Hospital approved this study and informed consent was obtained from all patients. Human fracture HCs were isolated and cultured as previously described (Oe et al, 2007;Hasegawa et al, 2009;Lee et al, 2013). Briefly, haematoma at the fracture site was harvested and minced into small pieces with growth medium [GM; α-modified minimum essential medium (α-MEM; Sigma, St. Louis, MO, USA) containing 10% heat-inactivated fetal bovine serum (FBS; Sigma), 2 mM L-glutamine (Gibco, Carlsbad, CA, USA) and antibiotics] in a culture dish.…”

Section: Methodsmentioning

confidence: 99%

“…HCs (7 × 10 4 cells/well) were seeded into a six-well plate and were cultured for 20 days in an osteogenic medium consisting of GM supplemented with 10 mM β-glycerophosphate (Sigma) and 50 μg/ml ascorbic acid (Wako, Osaka, Japan) (Hasegawa et al, 2007;Lee et al, 2013). For chondrogenic differentiation, a pellet culture was performed for three-dimensional (3D) culture.…”

Section: Osteogenic and Chondrogenic Inductionmentioning

confidence: 99%

“…At day 20, cells were fixed for 1 h with 95% ethanol and stained with 1% alizarin red S (Hartman Leddon, Philadelphia, PA, USA) (Oe et al, 2007;Hasegawa et al, 2009;Lee et al, 2013). Alizarin red S staining was released from the cell matrix by incubation in 10% ethylpyridinium chloride for 15 min, and the amount of dye released was quantified by spectrophotometry at 562 nm (Hasegawa et al, 2009).…”

Section: Mineralization Assay and Glycosaminoglycan (Gag) Depositionmentioning

confidence: 99%

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Effects of parathyroid hormone 1-34 on osteogenic and chondrogenic differentiation of human fracture haematoma-derived cellsin vitro

Dogaki

Lee

Niikura

et al. 2013

J Tissue Eng Regen Med

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Parathyroid hormone (PTH) 1-34 has been shown to accelerate fracture healing. Previously, we reported that progenitor cells with osteogenic and chondrogenic potential exist in human fracture haematoma, suggesting that the fracture haematoma-derived progenitor cells (HCs) contribute to fracture healing. However, there has been no study investigating the effect of PTH on HCs. We investigated the effect of pulsatile and continuous PTH treatment on human fracture HCs in vitro. HCs were isolated from seven patients. The HCs were divided into four groups: growth medium; control [osteogenic medium (OM) without PTH]; PTH-C (OM with continuous PTH); and PTH-P (OM with pulsatile PTH) groups. Osteogenic differentiation potential and proliferation of HCs were compared among the four groups. For chondrogenesis, the HCs were divided into two groups: control [chondrogenic medium (CM) without PTH]; and PTH-C (CM with continuous PTH) groups, and chondrogenic differentiation potential was analysed. PTH treatment did not affect cell proliferation, regardless of the mode of administration. Osteogenic activity was also not significantly affected by continuous PTH treatment but significantly inhibited by pulsatile PTH treatment. Conversely, chondrogenic differentiation was significantly inhibited by continuous PTH treatment. Our results revealed that PTH treatment on HCs, either continuous or pulsatile, does not exhibit any positive effect, and indicates that exogenous PTH administration after fracture has no effect on HCs. PTH may not have a positive effect at the fracture site during the early stage of fracture healing in which haematoma formation occurs. Copyright © 2013 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Section: Osteogenic and Chondrogenic Inductionmentioning

confidence: 99%

Section: Mineralization Assay and Glycosaminoglycan (Gag) Depositionmentioning

confidence: 99%

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Effects of parathyroid hormone 1-34 on osteogenic and chondrogenic differentiation of human fracture haematoma-derived cellsin vitro

Dogaki

Lee

Niikura

et al. 2013

J Tissue Eng Regen Med

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“…In differentiating murine osteoblasts, LIPUS has been found to enhance RANKL gene expression 10-fold compared to unstimulated controls after 3 weeks of LIPUS treatment [84]. It was also reported that LIPUS combined with growth factors such as calcium-regulating hormones, 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) [86], BMP2 [87], or BMP7 [88], can stimulate bone repair.…”

Section: Physical Stimulation For Bone Regeneration and Fracture Healingmentioning

confidence: 99%

Physical Stimulations for Bone and Cartilage Regeneration

Huang

Das

Patel

et al. 2018

Regen. Eng. Transl. Med.

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A wide range of techniques and methods are actively invented by clinicians and scientists who are dedicated to the field of musculoskeletal tissue regeneration. Biological, chemical, and physiological factors, which play key roles in musculoskeletal tissue development, have been extensively explored. However, physical stimulation is increasingly showing extreme importance in the processes of osteogenic and chondrogenic differentiation, proliferation and maturation through defined dose parameters including mode, frequency, magnitude, and duration of stimuli. Studies have shown manipulation of physical microenvironment is an indispensable strategy for the repair and regeneration of bone and cartilage, and biophysical cues could profoundly promote their regeneration. In this article, we review recent literature on utilization of physical stimulation, such as mechanical forces (cyclic strain, fluid shear stress, etc.), electrical and magnetic fields, ultrasound, shock waves, substrate stimuli, etc., to promote the repair and regeneration of bone and cartilage tissue. Emphasis is placed on the mechanism of cellular response and the potential clinical usage of these stimulations for bone and cartilage regeneration.

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“…We used a low-intensity pulsed US exposure device (Teijin Pharma Ltd, Tokyo, Japan) that was adapted for use with a 6-well culture plate in the in vitro experiments ( Figure 1). 17,[21][22][23] The thickness of the plate bottom was 1.5 mm. The volume of growth medium in each well was 2 mL.…”

Section: Low-intensity Pulsed Us Treatmentmentioning

confidence: 99%

Effect of Low‐Intensity Pulsed Ultrasound on Bone Morphogenetic Protein 7–Induced Osteogenic Differentiation of Human Nonunion Tissue‐Derived Cells In Vitro

Koga

Lee

Niikura

et al. 2013

J of Ultrasound Medicine

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Low-Intensity Pulsed Ultrasound Enhances BMP-7-Induced Osteogenic Differentiation of Human Fracture Hematoma-Derived Progenitor Cells In Vitro

Abstract: These findings demonstrated for the first time the significant effect of LIPUS on the osteogenic differentiation of HCs in the presence of BMP-7. This study may provide significant evidence for the clinical combined application of BMP-7 and LIPUS for the treatment of acute bone fractures.

Cited by 18 publications

References 30 publications

Effects of parathyroid hormone 1-34 on osteogenic and chondrogenic differentiation of human fracture haematoma-derived cellsin vitro

Effects of parathyroid hormone 1-34 on osteogenic and chondrogenic differentiation of human fracture haematoma-derived cellsin vitro

Physical Stimulations for Bone and Cartilage Regeneration

Effect of Low‐Intensity Pulsed Ultrasound on Bone Morphogenetic Protein 7–Induced Osteogenic Differentiation of Human Nonunion Tissue‐Derived Cells In Vitro

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