Low-Intensity Pulsed Ultrasound Reduces the Inflammatory Activity of Synovitis

Abstract

The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) on the cell proliferation and growth of synovial membrane cells stimulated with inflammatory cytokines, and to evaluate the effectiveness of LIPUS treatment of synovitis in the knee joints of animal models for rheumatoid arthritis. The rabbit knee synovial membrane cell line, HIG-82, was cultured in medium with or without IL-1β or TNF-α. Four hours after stimulation with the cytokines, the cells received LIPUS or sham exposure. Cell proliferation and growth were then analyzed. Using MRL/lpr mice, the anti-inflammatory effects of LIPUS were also evaluated in vivo. Stimulation with proinflammatory cytokines significantly up-regulated cell proliferation which was significantly down-regulated by LIPUS exposure. In MRL/lpr mice, exposure of knee joints to LIPUS caused a significant reduction of histological damage compared to the control. Histological lesions were significantly reduced in the joints treated with LIPUS for 3 weeks. Cox-2-positive cells in the knee joints treated with LIPUS were markedly decreased compared to the control joints. Therefore, LIPUS stimulation may be a medical treatment for joint inflammatory diseases, such as synovitis.

Hesperidin and Experimentally-Induced Arthritis in Male Rats

“…The current study revealed increased serum levels of both IL-1 and TNF-in the rheumatoid arthritis groups. These results run parallel to several previous reports that showed RA progression accelerated by pro-inflammatory cytokines (TNF-α and IL-1β) and chemokines (29,30) . In comparison to the arthritic control, the serum samples from the animals receiving hesperidin treatment demonstrated a significant drop in the levels of IL-1 and TNF-.…”

Ultrasound Modulates Skeletal Muscle Cytokine Levels in Rats with Heart Failure

“…Recently, some studies have reported on the influence of TU on inflammatory conditions in vivo (Signori et al 2011) and in vitro (Nakamura et al 2011). The results of our study indicate an important effect of TU on TNF-a and IL-6.…”

“…In recent years, some studies have reported the effectiveness of TU in the management of the inflammatory process (Nakamura et al 2011;Signori et al 2011). However, despite the positive effects of TU on the acute inflammatory profile, no studies have evaluated the effect of TU on the inflammatory process in a chronic heart failure model.…”

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

“…16,23,24,29 In addition, it has been demonstrated that LIPUS can inhibit osteoclast activity by decreasing the Receptor activator of nuclear factor kappa-B ligand/Osteoprotegerin (RANKL/OPG) ratio. 23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells. 32 Our findings are in accordance with previous reports 15,25,30,31 that showed significantly fewer RL in LIPUS-treated teeth compared to control teeth, except for at the distal surface.…”

“…23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells. 32 Our findings are in accordance with previous reports 15,25,30,31 that showed significantly fewer RL in LIPUS-treated teeth compared to control teeth, except for at the distal surface. The distal surface in the LIPUS group showed a lower number of RL compared to the control; however, this difference was not significant.…”