Low-Intensity Pulsed Ultrasound Therapy Stimulates Callus Formation between Host Femur and Cortical Onlay Strut Allograft

Akiyama, Haruhiko; Hachiya, Yudo; Otsuka, Hiromi; Kurisuno, Makoto; Kawanabe, Keiichi; Katayama, Naoyuki; Ohura, Hisanori; Yamamoto, Kōji; Sato, Keiji; Matsuda, Shuichi

doi:10.1016/j.ultrasmedbio.2013.12.030

Cited by 7 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the use of ultrasound in the DBM group with the profile of 1 MHz and intensity of 30 mW/cm 2 and 20 minutes/day scheme, caused a slight (non-significant) delay in the healing process. This finding is in contrast with stud- ies that showed a positive effect of ultrasound on fracture healing and osseous implants (12,26). However, our results are similar with the findings of other researchers, who showed no effect for ultrasound on fracture healing such as Thus, the ultrasonic effect might only influence cells on the periosteal surface and in the outermost layers of the bone cortex.…”

Section: Discussionsupporting

confidence: 79%

“…In this study the effect of DBM in combination with LI-PUS treatment was investigated on tibial fracture healing in a rabbit model. Use of LIPUS for treatment of diseases was investigated in the last two decades and defined as an effective method in several studies (12)(13)(14). Due to hazardous effect of high intensity ultrasound (US) on osseous tissue and fracture healing, low intensity US was used in the present study (15).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Low Intensity Pulsed Ultrasound Effects on the Osteogenesis Potential of Demineralized Bone Matrix in Experimental Tibial Defect in Rabbits

Givi

Baniadam

Esmaeilzadeh

et al. 2016

Jentashapir J Health Res

View full text Add to dashboard Cite

Background: Many studies have assessed the effects of either low intensity pulsed ultrasound (LIPUS) or demineralized bone matrix (DBM) on bone repair; however, an evaluation of the combination of these modalities (LIPUS + DBM) has not yet been considered.Objectives: This study aimed to investigate combined effects of DBM and LIPUS on fracture healing. Methods: Bilateral 5-mm tibial defects were created in male Dutch rabbits (n = 30). Animals were divided to two groups of empty defect (A) and DBM group (B), in which commercial DBM putty was used in defects. In each animal left tibia was treated with LIPUS (intensity = 30 mW/cm 2 , I SATA, 1 MHz, 20 min/day, pulsed duty 1:4) and the contralateral limb was used as the control. Animals, after 14, 28 and 60 days, were submitted to radiographic or computerized tomography (CT) scanning analysis.Results: At two weeks, LIPUS had no substantial effect on bone formation. Slight increase of average rates in LIPUS group (A2) were seen compared to the empty defect group (A1) at day 21 and 28. In the DBM-treated group compared with the sham LIPUS, bone-healing rate was reduced at the end of the period (60 days) after surgery. The average healing rate in group B at the end of the 60-day period was less than group A after 21 days. Conclusions:The present study discusses systemic effect of LIPUS because of non-significant results between treated group and control group and is the first to demonstrate that LIPUS decreases bone formation induced by DBM.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Evaluation of Low Intensity Pulsed Ultrasound Effects on the Osteogenesis Potential of Demineralized Bone Matrix in Experimental Tibial Defect in Rabbits

Givi

Baniadam

Esmaeilzadeh

et al. 2016

Jentashapir J Health Res

View full text Add to dashboard Cite

show abstract

“…LIPUS is a clinically established, widely used and FDA approved therapy to enhance bone growth during healing of non-union, fractures and other osseous defects [ 28 , 48 ]. LIPUS induces mechanical stress in bone that, in turn, stimulates ossification of a soft callus through the modulation of calcium ion channels [ 49 – 52 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells

Carina

Costa

Pagani

et al. 2018

J Exp Clin Cancer Res

View full text Add to dashboard Cite

BackgroundBone tissue is one of the main sites for breast metastasis; patients diagnosed with advanced breast cancer mostly develop bone metastasis characterized by severe osteolytic lesions, which heavily influence their life quality. Low Intensity Pulsed Ultrasound (LIPUS) is a form of mechanical energy able to modulate various molecular pathways both in cancer and in health cells.The purpose of the present study was to evaluate for the first time, the ability of LIPUS to modulate osteolytic capability of breast cancer cells.MethodsTwo different approaches were employed: a) Indirect method -conditioned medium obtained by MDA-MB-231 cell line treated or untreated with LIPUS was used to induce osteoclast differentiation of murine macrophage Raw264.7 cell line; and b) Direct method -MDA-MB-231 were co-cultured with Raw264.7 cells and treated or untreated with LIPUS.ResultsLIPUS treatment impaired MDA-MB-231 cell dependentosteoclast differentiation and produced a reduction of osteoclast markers such as Cathepsin K, Matrix Metalloproteinase 9 and Tartrate Resistant Acid Phosphatase, suggesting its role as an effective and safe adjuvant in bone metastasis management.ConclusionLIPUS treatment could be a good and safety therapeutic adjuvant in osteolyitic bone metastasis not only for the induction properties of bone regeneration, but also for the reduction of osteolysis.

show abstract

“…This might prevent some potential candidates from receiving non-invasive treatment strategies such as LIPUS. Furthermore, a large number of studies dedicated to LIPUS stimulation of fresh fractures are either based on case studies (data not shown), retrospective studies (Akiyama et al, 2014;Arima et al, 2017;Kinami et al, 2013;Ota et al, 2018;Ota et al, 2017;Song et al, 2019;Zura et al, 2015b) or prospective trials conducted in an unblinded manner and/or without sham controls (Arimoto et al, 2019;Brand et al, 1999;Dudda et al, 2011;El-Mowafi and Mohsen, 2005;Gan et al, 2014;Gold and Wasserman, 2005;Gopalan et al, 2020;Leung et al, 2004b;Liu et al, 2014;Patel et al, 2015;Salem and Schmelz, 2014;Santana-Rodríguez et al, 2019;Tsumaki et al, 2004;Urita et al, 2013) (Table 1), challenging the credibility of the LIPUS therapy. Additionally, the small size of patient cohorts of several prospective, randomised, double-blind, placebo-controlled trials diminish the importance of their findings (Emami et al, 1999;Handolin et al, 2005a;Handolin et al, 2005b;Raza et al, 2016).…”

Section: Lipus and Fresh Fractures: Surgery Vs Castmentioning

confidence: 99%

Pulsed ultrasound for bone regeneration – outcomes and hurdles in the clinical application: a systematic review

Puts

Vico²,

Beilfuss³

et al. 2021

eCM

View full text Add to dashboard Cite

Impaired bone-fracture healing is associated with long-term musculoskeletal disability, pain and psychological distress. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive and side-effect-free treatment option for fresh, delayed- and non-union bone fractures, which has been used in patients since the early 1990s. Several clinical studies, however, have questioned the usefulness of the LIPUS treatment for the regeneration of long bones, including those with a compromised healing. This systematic review addresses the hurdles that the clinical application of LIPUS encounters. Low patient compliance might disguise the effects of the LIPUS therapy, as observed in several studies. Furthermore, large discrepancies in results, showing profound LIPUS effects in regeneration of small-animal bones in comparison to the clinical studies, could be caused by the suboptimal parameters of the clinical set-up. This raises the question of whether the so-called “acoustic dose” requires a thorough characterisation to reveal the mechanisms of the therapy. The adequate definition of the acoustic dose is especially important in the elderly population and patients with underlying medical conditions, where distinct biological signatures lead to a delayed regeneration. Non-industry-funded, randomised, double-blind, placebo-controlled clinical trials of the LIPUS application alone and as an adjuvant treatment for bones with complicated healing, where consistent control of patient compliance is ensured, are required.

show abstract

Low-Intensity Pulsed Ultrasound Therapy Stimulates Callus Formation between Host Femur and Cortical Onlay Strut Allograft

Cited by 7 publications

References 24 publications

Evaluation of Low Intensity Pulsed Ultrasound Effects on the Osteogenesis Potential of Demineralized Bone Matrix in Experimental Tibial Defect in Rabbits

Evaluation of Low Intensity Pulsed Ultrasound Effects on the Osteogenesis Potential of Demineralized Bone Matrix in Experimental Tibial Defect in Rabbits

Inhibitory effects of low intensity pulsed ultrasound on osteoclastogenesis induced in vitro by breast cancer cells

Pulsed ultrasound for bone regeneration – outcomes and hurdles in the clinical application: a systematic review

Contact Info

Product

Resources

About