Low level laser therapy (LLLT) modulates ovarian function in mature female mice

Abstract

Low level laser therapy (LLLT) modulates ovarian function in mature female mice, Progress in (2018), ABSTRACT 22 It is known that LLLT has beneficial effects on several pathological conditions including 23 wound healing, pain and inflammation. LLLT modulates biological processes, including 24 cell proliferation, apoptosis and angiogenesis. In the present study, we examined the effect 25 of local application of LLLT on follicular dynamics, ovarian reserve, AMH expression, 26 progesterone levels, apoptosis, angiogenesis, and reproductive outcome in adult mice. 27 LLLT (200 J/cm 2 ) increased the percentage of primary and preantral follicles, whilst 28 decreasing the percentage of corpora lutea compared to control ovaries. LLLT-treated 29 ovaries did not exhibit any changes regarding the number of primordial follicles. We 30 observed a higher percentage of AMH-positive follicles (in early stages of development) in 31 LLLT-treated ovaries compared to control ovaries. LLLT reduced the P 4 concentration and 32 the apoptosis in early antral follicles compared to control ones. LLLT caused a reduction in 33 the endothelial cell area and an increase in the periendothelial cell area in the ovary. 34 Additionally, LLLT was able to improve oocyte quality. Our findings suggest that local 35 application of LLLT modulates follicular dynamics by regulating apoptosis and the 36 vascular stability in mouse ovary. In conclusion, these data indicate that LLLT might 37 become a novel and useful tool in the treatment of several pathologies, including female 38 reproductive disorders.39 40

Biological therapies for premature ovarian insufficiency: what is the evidence?

“…Finally, photobiomodulation therapy (PBMT) with low-level laser light therapy (LLLT) is known to exert its rejuvenative effects via targeting the chromophore cytochrome C oxidase in mitochondrial membrane (119). In two rodent studies, PBMT is shown to increase the number of primary and pre-antral follicle count (120,121), to decrease granulosa cell apoptosis and to increase angiogenesis; however, it was not shown to increase the number of Pfs (120).…”