Low-molecular-weight sclera protein biologically active at ultralow doses

Experimental Models for Culturing of Eye Tissues of Pleurodeles Waltl for Evaluation of Specific Effects of Sclera Bioregulator in Ultra-Low Doses

“…They constitute a new group of protein compounds characterized by the capacity to modulate vital biological processes (migration, adhesion, proliferation, and cell differentiation) in ULD. We previously found a bioregulator active in ULD in bovine sclera [2]. By its physicochemical properties it corresponded to this group of bioregulators.…”

“…Bioregulators active in ULD were found in various mammalian tissues [1][2][3][4][5][6][7][8][9]. They constitute a new group of protein compounds characterized by the capacity to modulate vital biological processes (migration, adhesion, proliferation, and cell differentiation) in ULD.…”

“…The fraction of sclera bioregulator in a concentration of 10 -9 mg/ml was added to vials of experimental series before culturing. Sclera bioregulator was isolated from cattle sclera tissue using a previously developed approach [2]. Previously developed method of biotesting [4] was used at each stage of purifi cation to identify the bioregulator in fractions.…”

Cryostructuring of Polymeric Systems. 57. Spongy Wide-Porous Cryogels Based on the Proteins of Blood Serum: Preparation, Properties and Application as the Carriers of Peptide Bioregulators

“…To this end, sponge-like proteinaceous cryogels were prepared, originating from the total proteins of bovine blood serum; then, some physico-chemical characteristics of such materials and their macroporous morphology were evaluated followed by loading of the cryogels with the bioregulators isolated either from the serum of bovine blood [45] or from the sclera of bovine eyes [46]. The subsequent biological testing of the delivery matrices thus obtained was accomplished using the earlier developed biological model [47].…”