Low oxygen stimulates proliferation of fibroblasts seeded as single cells

Abstract

In standard tissue culture conditions (20% oxygen), single human dermal fibroblasts (one cell per well) do not proliferate. We now report that low oxygen tension is a potent stimulus for the proliferation and expansion of human adult and neonatal dermal fibroblasts seeded as single cells. This preferential single-cell proliferation in low oxygen is shown to be also a feature of human lung and dermal rodent fibroblasts, but not of human fibrosarcoma and immortalized 3T3 cells, which proliferate without difficulty in standard oxygen conditions. It is suggested that single-cell proliferation and its dramatic stimulation in low oxygen may represent a fundamental biologic process with an opportunity to better understand mammalian cell growth regulation.

Efficient TALEN-mediated gene knockout in livestock

“…In addition, selection is an extremely reliable method for clonal isolation in primary fibroblasts, a cell type averse to low culture density and dilutional cloning (24).…”

Oxygen in wound healing: More than a nutrient

“…After 72 hours exposure to 1% oxygen, the proliferative rate of fibroblasts increases by 71% [10]. Low oxygen tensions have been shown to initiate clonal expansion of fibroblasts [18], a finding that would be surprising if only the previously made statements in this review were taken into account.…”

Effect of Oxygen Tension on the Developmental Potential of Parthenogenetic Oocytes and Nuclear-Transferred Porcine Oocytes Receiving Fetal Fibroblast Cells.

“…The potential of nuclear-transferred oocytes receiving somatic cells cultured under low oxygen tension to develop to the 2-cell, 8-cell, and morula stages was not significantly different, but d e v e l o p m e n t t o t h e b l a s t o c y s t s t a g e w a s significantly lower for oocytes receiving donor cells cultured under high oxygen tension, irrespective of the oxygen tension during embryo culture. The overall proportions of nuclear-transferred oocytes (27) 14 (7) 14 (7) (7) 15 (5) 22.0 ± 8.7 (14) Values with diferent superscripts in the same column differ significantly (p<0.05). 1) High indicates 5% CO2 in air.…”

“…This might be due to the fact that in vitro maturation and culture media for porcine oocytes in recent reports include cysteine or cysteamine [11,12], which increase glutathione concentration in oocytes and protects cells from oxidative damage [13]. Although several reports demonstrated that somatic cells grew better when cultured under low oxygen tension [14,15], recent procedures for in vitro culture of somatic cells for nuclear transfer have used mainly atmospheric o x y g e n t e n s i o n ( 2 0 % ) . A r e c e n t s t u d y demonstrated that high concentrations of hydrogen peroxide, which produces high oxygen tension, induced necrosis of human lung fibroblasts in culture [16].…”