To improve the accuracy of stress detection and stress warning errors, this paper designed a new psychological stress detection and warning system based on wireless network transmission. To achieve this objective, we established a real-time setup. The hardware of the system is composed of pulse acquisition module, signal low-pass filtering and amplification module, real-time clock module, wireless network transmission module, and power supply module, respectively. Based on the aforementioned hardware platform, the system software is designed, mainly through the construction of pulse signal noise and the use of wavelet denoising threshold for sensing wavelet packet inverse transformation, to get the reconstructed signal. The peak point of the reconstructed signal is determined, and the value of pulse signal is extracted. According to the characteristic value’s extraction results, the degree of psychological stress is quantified using the psychological stress index (PSI). When the PSI exceeds a predefined threshold, it indicates an early warning of psychological stress. The experimental results show that the psychological stress detection of our system is consistent with the expert evaluation results, the warning time is short, and the practical application effect is good.