Low‐power laser stimulation of tissue engineered cartilage tissue formed on a porous calcium polyphosphate scaffold

Gan, Lu; Tse, Carmen; Pilliar, Robert M.; Kandel, Rita A.

doi:10.1002/lsm.20471

Cited by 8 publications

(3 citation statements)

References 46 publications

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“…On day 5, the serum concentration was increased to 20%, and the medium was supplemented with ascorbic acid (100 mg/mL; Sigma Chemical Co.). 5,13,35,37 At this time, cultures were incubated in the presence or absence of various concentrations of sodium phosphate glasses (inorganic polyphosphate; average chain lengths of 5, 45, and 75 phosphate residues; Sigma Chemical Co.) or sodium phosphate buffer. The concentrations of polyphosphate utilized for each experiment are calculated based on the phosphate content.…”

Section: Tissue Culturementioning

confidence: 99%

“…6 Our group and others have shown that mechanical stimulation of in vitroformed cartilage increases extracellular matrix accumulation and yields improved tissue mechanical properties. 4,[7][8][9][10] Other techniques such as the application of ultrasound, 11,12 laser stimulation, 13 as well as changes in the culture conditions such as oxygen tension 14,15 and temperature 16 have all resulted in modest improvements in matrix accumulation. Despite these efforts, in vitro-formed cartilage remains qualitatively and functionally inferior to native articular cartilage and our understanding of the signals that stimulate extracellular matrix deposition by chondrocytes is still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Inorganic Polyphosphate Stimulates Cartilage Tissue Formation

St-Pierre

Wang

et al. 2012

Tissue Engineering Part A

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Clinical utilization of tissue-engineered cartilage constructs has been limited by their inferior mechanical properties compared to native articular cartilage. A number of strategies have been investigated to increase the accumulation of major extracellular matrix components within in vitro-formed cartilage, including the administration of growth factors and mechanical stimulation. In this study, the anabolic effect of inorganic polyphosphates, a linear polymer of orthophosphate residues linked by phosphoanhydride bonds, was demonstrated in both chondrocyte cultures and native articular cartilage cultured ex vivo. Compared to untreated controls, polyphosphate treatment of three-dimensional primary chondrocyte cultures induced increased glycosaminoglycan and collagen accumulation in a concentration- and chain length-dependent manner. This effect was transient, because chondrocytes express exopolyphosphatases that hydrolyze polyphosphate. The anabolic effect of polyphosphates was accompanied by a lower rate of DNA increase within the chondrocyte cultures treated with inorganic polyphosphate. Inorganic polyphosphate enhances cartilage matrix accumulation and is a promising approach to improve the quality of tissue-engineered cartilage constructs.

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Section: Tissue Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inorganic Polyphosphate Stimulates Cartilage Tissue Formation

St-Pierre

Wang

et al. 2012

Tissue Engineering Part A

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“…It was demonstrated that CPP showed good property in vitro along with attached marrow-derived mesenchymal cells for transplantation into a site for bone regeneration in vivo (Lee et al, 2001). And in the recent years, some researchers have found that the chondrocytes would not get calcification in the porous material, but showed good appearance and cellularity (Gan, Tse, Pilliar, & Kandel, 2007).…”

Section: Introductionmentioning

confidence: 98%

Evaluation of novel alginate dialdehyde cross-linked chitosan/calcium polyphosphate composite scaffolds for meniscus tissue engineering

Wang

Zhang

et al. 2010

Carbohydrate Polymers

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Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source

Çakmak

Vatansever

et al. 2017

Lasers Med Sci

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Low-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590-1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration.

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Low‐power laser stimulation of tissue engineered cartilage tissue formed on a porous calcium polyphosphate scaffold

Abstract: Short exposures to low-power laser stimulation using a laser diode with 3 J/cm(2) dose improves cartilage tissue formation.

Cited by 8 publications

References 46 publications

Inorganic Polyphosphate Stimulates Cartilage Tissue Formation

Inorganic Polyphosphate Stimulates Cartilage Tissue Formation

Evaluation of novel alginate dialdehyde cross-linked chitosan/calcium polyphosphate composite scaffolds for meniscus tissue engineering

Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source

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