Low Prevalence of Human Papilloma Virus in Patients with Breast Cancer, Kerman; Iran

Afshar, Reza Malekpour; Balar, Negin; Mollaei, Hamid Reza; Arabzadeh, Seyed Alimohammad; Iranpour, Maryam

doi:10.31557/apjcp.2018.19.11.3039

Cited by 22 publications

(16 citation statements)

References 20 publications

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“…The present results indicated that 53% of the identified HPVs belonged to the high-risk group. This finding is consistent with previous studies by Ngamkham et al, (2017);Malekpour Afshar et al, (2018) and Sigaroodi et al, (2012) indicating high-risk genotypes as the predominant type in breast tissue samples.…”

Section: Resultssupporting

confidence: 94%

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Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens

Alinezhadi

Makvandi

Kaydani

et al. 2022

Asian Pac J Cancer Prev

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Background: According to several studies, there is an association between human papillomavirus (HPV) and breast cancer. Therefore, detection and genotyping of HPV seem important. The present study aimed to investigate the presence of HPV DNA in breast tissues by analyzing the L1 gene. Materials and Methods: This case-control study was conducted on 63 formalin-fixed paraffin-embedded (FFPE) tissues of invasive ductal carcinoma (IDC) as the case group and 32 FFPE tissues of fibroadenoma as the control group. HPV DNA was detected using the polymerase chain reaction assay. Positive samples were then subjected to genotyping. All statistical analyses were performed in SPSS version 22.0. Results: The patients' age ranged from 15 to 92 years, with a mean age of 43.54±16.36 years. HPV DNA was detected in 17/95 (17.89%) samples, including 9/32 (28.12%) fibroadenoma samples and 8/63 (12.69%) IDC samples. No significant difference was observed regarding the presence of HPV DNA between the IDC and fibroadenoma tissues (P=0.08). However, a significant difference was found in the detection of high-risk HPV (HR-HPV) between the case and control groups (P=0.03). In the case group, 87.5% of the detected viruses (7/8 samples) were HR-HPV, while in the control group, 22.22% of positive samples (2/9 samples) were HR-HPV (P=0.03). Based on the results, HR-HPV and low-risk HPV genotypes were detected in 53% (9/17) and 47% (8/17) of positive samples, respectively. Conclusion: In this study, 12.69% of IDC samples were positive for HPV genomes, and HR-HPV was detected in 87.5% of these samples. The present results suggest the important role of HR-HPV in the development of breast cancer.

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Section: Resultssupporting

confidence: 94%

“…Different rates of HPV have been reported among BC patients in different parts of Iran. In agreement with our findings, a study by Malekpour Afshar et al, (2018), detected HPV DNA in 8.2% (8/98) of BC patients. Overall, 62.5% and 37.5% of positive samples were related to oncogenic genotypes 16/18 and 31/33, respectively.…”

Section: Resultssupporting

confidence: 93%

Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens

Alinezhadi

Makvandi

Kaydani

et al. 2022

Asian Pac J Cancer Prev

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show abstract

“…However, a study conducted in Indian women with breast cancer found no traces of either HPV DNA or E6 and E7 proteins, both, by conventional and real time PCR ( Hedau et al, 2011 ). Similarly, another study done on patients with breast cancer in Iran found DNA of HPV in only eight out of the 98 patient samples, which is an extremely low prevalence; among HPV positive samples, 62.5% of DNA was of HPV 16 and 18 ( Malekpour Afshar et al, 2018 ). In addition, Kwong et al (2013) found no traces of HPV by PCR in their study of 102 breast cancer patient samples in Hong Kong.…”

Section: Discussionmentioning

confidence: 86%

Interrelated Oncogenic Viruses and Breast Cancer

Afzal

Fiaz

Noor

et al. 2022

Front. Mol. Biosci.

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Breast Cancer is a multifactorial disease and recent evidence that viruses have a greater role in its aetiology and pathophysiology than previously hypothesized, has garnered a lot of attention in the past couple of years. After the role of Mouse Mammary Tumour Virus (MMTV) in the oncogenesis of breast cancer has been proved in mice, search for similar viruses found quite a plausible relation of Human Papilloma Virus (HPV), Epstein–Barr virus (EBV), and Bovine Leukaemia Virus (BLV) with breast cancer. However, despite practical efforts to provide some clarity in this issue, the evidence that viruses cause breast cancer still remains inconclusive. Therefore, this article aims to clarify some ambiguity and elucidate the correlation of breast cancer and those particular viruses which are found to bring about the development of tumorigenesis by a previous infection or by their own oncogenic ability to manipulate the molecular mechanisms and bypass the immune system of the human body. Although many studies have reported, both, the individual and co-existing presence of HPV, EBV, MMTV, and BLV in patient sample tissues, particularly in Western women, and proposed oncogenic mechanisms, majority of the collective survey of literature fails to provide a delineated and strong conclusive evidence that viruses do, in fact, cause breast cancer. Measures to prevent these viral infections may curb breast cancer cases, especially in the West. More studies are needed to provide a definite conclusion.

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“…In order to investigate JCPyV miRNA expression, the reverse-transcribed RNA was used for PCR amplification of miR-J1-5p coding region (Giovannelli et al 2015). DNA and cDNA were further analyzed for cellular p53 by PCR (Malekpour Afshar et al 2016). JCPyV DNA was detected with a viral load value of 6.0 × 10 4 gEq/mL.…”

Section: Case Presentationmentioning

confidence: 99%

Detection of human neurotropic JCPyV DNA sequence in pediatric anaplastic xanthoastrocytoma

et al. 2023

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Due to its peculiar histopathological findings, pleomorphic xanthoastrocytoma (PXA), a rare cerebral tumor of young adults with a slow growth and a good prognosis, resembles to the lytic phase of progressive multifocal leukoencephalopathy, a fatal neurodegenerative disease caused by JC polyomavirus (JCPyV). Therefore, the presence of JCPyV DNA was examined in an 11-year-old child with xanthoastrocytoma, WHO grade 3, by quantitative PCR (qPCR) and nested PCR (nPCR) using primers amplifying sequences encoding the N- and C-terminal region of large T antigen (LTAg), the non-coding control region (NCCR), and viral protein 1 (VP1) DNA. The expression of transcripts from LTAg and VP1 genes was also evaluated. In addition, viral microRNAs’ (miRNAs) expression was investigated. Cellular p53 was also searched at both DNA and RNA level. qPCR revealed the presence of JCPyV DNA with a mean value of 6.0 × 104 gEq/mL. nPCR gave a positive result for the 5ʹ region of the LTAg gene and the NCCR, whereas 3ʹ end LTAg and VP1 DNA sequences were not amplifiable. Only LTAg transcripts of 5ʹ end were found whereas VP1 gene transcript was undetectable. Although in most cases, either Mad-1 or Mad-4 NCCRs have been identified in association with JCPyV-positive human brain neoplasms, the archetype NCCR structure was observed in the patient’s sample. Neither viral miRNA miR-J1-5p nor p53 DNA and RNA were detected. Although the expression of LTAg supports the possible role of JCPyV in PXA, further studies are warranted to better understand whether the genesis of xanthoastrocytoma could depend on the transformation capacity of LTAg by Rb sequestration.

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Low Prevalence of Human Papilloma Virus in Patients with Breast Cancer, Kerman; Iran

Cited by 22 publications

References 20 publications

Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens

Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens

Interrelated Oncogenic Viruses and Breast Cancer

Detection of human neurotropic JCPyV DNA sequence in pediatric anaplastic xanthoastrocytoma

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