Low Salivary Amylase Gene (AMY1) Copy Number Is Associated with Obesity and Gut Prevotella Abundance in Mexican Children and Adults

León-Mimila, Paola; Villamil‐Ramírez, Hugo; López-Contreras, Blanca E.; Morán-Ramos, Sofía; Macías-Kauffer, Luis; Acuña-Alonzo, Víctor; Río-Navarro, Blanca Estela Del; Salmerón, Jorge; Velázquez‐Cruz, Rafael; Villarreal-Molina, Teresa; Aguilar-Salinas, Carlos A.; Canizales‐Quinteros, Samuel

doi:10.3390/nu10111607

Cited by 39 publications

(43 citation statements)

References 57 publications

(89 reference statements)

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“…Although we had the same division for the low AMY1 group, their high AMY1 group included only participants with ≥8 copy numbers. As overweight/obese subjects have lower AMY1 copy numbers, [ 2,6,8 ] this could explain the lower median observed in our population.…”

Section: Discussionmentioning

confidence: 84%

“…[ 2,4 ] Hence, reduced AMY1 copy numbers have also been associated with increased body mass index (BMI), obesity and cardiovascular risk, IR, and inflammation in both adults and children. [ 2–8 ] However, the association between the AMY1 copy number and obesity has been challenged by recent studies that have found no significant associations despite having a strong statistical power. [ 9–12 ] These conflicting results could be partially attributed to differences in the starch intake.…”

Section: Introductionmentioning

confidence: 99%

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Low AMY1 Copy Number Is Cross‐Sectionally Associated to an Inflammation‐Related Lipidomics Signature in Overweight and Obese Individuals

Mayneris‐Perxachs

Mousa

Naderpoor

et al. 2020

Molecular Nutrition Food Res

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Scope Reduced amylase 1 (AMY1) copy numbers are associated with obesity, insulin resistance, and inflammation. Although mechanisms linking AMY1 copy number with metabolic disorders are poorly understood, recent findings suggest that lipids play a key role. Methods and results Plasma lipidomic signatures associated with AMY1 copy number are explored in 57 non‐diabetic overweight/obese subjects aged 18–60. Serum amylase and inflammatory cytokines levels are also measured. AMY1 copy number is strongly associated with the serum amylase concentration. Participants are divided into low‐(≤4) and high‐(>4) AMY1 carriers based on the median. Low‐AMY1 carriers have higher BMI and fat mass. They also have higher levels of dihexosylceramides (R = −0.27, p = 0.044), cholesterol esters (CE) (R = −0.32, p = 0.020), alkylphosphatidylcholines [PC(O)] (R = −0.33, p = 0.014), and sphingomyelins (SM) (R = −0.38, p = 0.005). From 459 lipid species, 28 differ between low‐ and high‐AMY1 carriers. These include CE species with long‐chain PUFA; PC(O) and PC plasmalogens containing arachidonic acid; and PC, mono‐, di‐, and tri‐hexosylceramides, and SM containing saturated fatty acids (mainly C16:0 and C20:0). This lipidomic signature is strongly associated with inflammatory cytokines, which are also negatively associated with the AMY1 copy number. Conclusion A lipidomics signature associated with low AMY1 copy numbers is revealed, which is linked to obesity and chronic low‐grade inflammation.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Low AMY1 Copy Number Is Cross‐Sectionally Associated to an Inflammation‐Related Lipidomics Signature in Overweight and Obese Individuals

Mayneris‐Perxachs

Mousa

Naderpoor

et al. 2020

Molecular Nutrition Food Res

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“…9,10 The genetic connection between AMY1 CNV and obesity showed that low AMY1 copy number significantly associated with high BMI. 4,5,[11][12][13] Recently from our lab, we showed the inverse association of AMY1 copy number and the obesity measurement in children. 13 Falchi et al first reported the association of AMY1 CNV and the high risk of obesity in a longitudinal study.…”

Section: Introductionmentioning

confidence: 95%

<p>Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children</p>

Selvaraju¹,

Venkatapoorna²,

Babu³

et al. 2020

DMSO

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Background: We have recently shown that the copy number of salivary amylase (AMY1) gene was significantly decreased, and the obesity-related salivary biomarkers resistin, MCP-1, TNF-α, IL-6, and CRP were significantly increased in overweight/obese children compared to normal weight. This study aimed to evaluate the association of AMY1 copy number variant (CNV) with obesity and inflammatory markers. Seventy-six participants aged between 6 and 10 years have participated, and the saliva samples were collected along with the anthropometric measurements. Methods: AMY1 copy number was analyzed by 3D digital PCR, and obesity-related biomarkers were performed with a Bioplex multiplex analyzer. Results: The mean AMY1 copy number was higher in normal weight (7.90 ± 0.38) compared to the overweight/obese group (6.20 ± 0.29). The association of AMY1 CNV with obesity and inflammatory markers showed significant negative correlation [CRP, β = −0.238 (p < 0.05); resistin, β = −0.25 (p < 0.05); MCP-1, β = −0.304 (p < 0.01)] except for complement factor D, TNF α and IL-6. The anti-inflammatory cytokine, IL-10 reported a positive correlation with AMY1 copy number with a β = 0.268 (p < 0.05). The multivariable model adjusted with age and gender depicted a similar correlation with obesity markers. Conclusion: Our results report that AMY1 CNV is associated with obesity and inflammatory biomarkers in children's saliva sample.

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“…body mass index (BMI) and waist circumference 7,[12][13][14][15][16][17][18][19][20][21] . However, other studies reported no association [22][23][24] or a positive association between AMY1 gene CN and BMI 25 .…”

mentioning

confidence: 99%

High plasma salivary α-amylase, but not high AMY1 copy number, associated with low obesity rate in Qatari adults: cross-sectional study

Al-Akl

Thompson

Arredouani

2020

Sci Rep

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The relationship between salivary α-amylase activity (psAAa) or AMY1 copy number and the risk of obesity remains controversial. We aimed to assess this relationship in a cohort from Qatar, where obesity affects 43% of adults. The relationship was investigated cross-sectionally in 923 Qatari adults from the Qatar biobank cohort. AMY1 CN was estimated form whole genome sequencing data. The associations with obesity prevalence were assessed by linear and logistic regressions. We found no difference in AMY1 CN between obese and normal-weight individuals. However, the psAAa was significantly lower in obese individuals. Significant inverse correlations were found between adiposity markers and psAAa in both sexes, but were marginally stronger in men. A significant effect of high psAAa, but not AMY1 CN, on reduced obesity rates was identified in men (OR per psAAa unit 0.957 [95% CI 0.937–0.977], p < 0.001, with psAAa ranging between 5 to 66 U/L). A significantly higher prevalence of obesity was observed in the lowest quartile of psAAa in men (75% (Q1) vs. 36% (Q4), p < 0.001) and women (74% (Q1) vs 56% (Q4), p = 0.009). Our findings suggest that high psAAa, but not AMY1 CN, has a potential positive benefit against obesity in the Qatari population.

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Low Salivary Amylase Gene (AMY1) Copy Number Is Associated with Obesity and Gut Prevotella Abundance in Mexican Children and Adults

Cited by 39 publications

References 57 publications

Low AMY1 Copy Number Is Cross‐Sectionally Associated to an Inflammation‐Related Lipidomics Signature in Overweight and Obese Individuals

Low AMY1 Copy Number Is Cross‐Sectionally Associated to an Inflammation‐Related Lipidomics Signature in Overweight and Obese Individuals

<p>Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children</p>

High plasma salivary α-amylase, but not high AMY1 copy number, associated with low obesity rate in Qatari adults: cross-sectional study

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