Low temperature bonding of poly(methylmethacrylate) electrophoresis microchips by in situ polymerisation

Chen, Gang; Li, Jianhua; Qu, Song; Chen, Di; Yang, Penyuan

doi:10.1016/j.chroma.2005.07.093

Cited by 34 publications

(30 citation statements)

References 35 publications

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“…A variety of techniques have been developed for bonding homogeneous or heterogeneous polymer pairings by exposing them to heat 25,26 , microwaves 27 , or solvents 28,29 . Other approaches have used glue 30 , or in situ polymerisation 31 for bonding. Several of these techniques have been tried in the course of this work, but were found to be unsuitable, producing either uneven bonds, or leading to buckling and warping of the microfluidic LoC device.…”

Section: Chip Qualitymentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

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A compact microfludic device for perfusion culture of mammalian cells under in-situ metabolomic observation by NMR spectroscopy is presented. The chip is made from poly(methyl methacrylate) (PMMA), and uses a poly(dimethyl siloxane) (PDMS) membrane to allow gas exchange. It is integrated with a generic micro-NMR detector developed recently in our group [J. Magn. Reson. 262, 73-80 (2016)]. While PMMA is an excellent material in the context of NMR, PDMS is known to produce strong background signals. To mitigate this, the device keeps the PDMS away from the detection area. The oxygen permeation into the device is quantified using a flow chemistry approach. A solution of glucose is mixed on the chip with one of glucose oxidase, before flowing through the gas exchanger. The resulting concentration of gluconate is measured by 1 H NMR spectroscopy as a function of flow rate. An oxygen equilibration rate constant of 2.4 s −1 is found for the device, easily sufficient to maintain normoxic conditions in a cell culture at modest perfusion flow rates.

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Section: Chip Qualitymentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

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Section: Characteristics Of the Microchipmentioning

confidence: 99%

“…8,16,[24][25][26][27] Based on the signal-to-noise ratio of 3, the LOD for Na + was 1.25 mg ml À1 by using our system. As expected, the LOD obtained by our chip is lower than those results obtained from chips with similar electrode geometry, thicker insulating layers and operated at low excitation frequencies, [24][25][26] as shown in Table 1. While it is higher than the results reported by Kubaň and Hauser,8,16,27 the excitation frequencies used in their work (500 kHz, 8 530 kHz, 16 600 kHz 27 ) are much higher than our applied frequency of 60 kHz and the range of the detector (0-300 kHz) used here, which is in line with the conclusion that the output of the detector increased sharply between 400 and 600 kHz.…”

Section: Characteristics Of the Microchipmentioning

confidence: 99%

A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection

et al. 2011

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A three-layer poly (methyl methacrylate) (PMMA) electrophoresis microchip integrated with Pt microelectrodes for contactless conductivity detection is presented. A 50 mm-thick PMMA film is used as the insulating layer and placed between the channel plate (containing the microchannel) and the electrode plate (containing the microelectrode). The three-layer structure facilitates the achievement of a thin insulating layer, obviates the difficulty of integrating microelectrodes on a thin film, and does not compromise the integration of microchips. To overcome the thermal and chemical incompatibilities of polymers and photolithographic techniques, a modified lift-off process was developed to integrate Pt microelectrodes onto the PMMA substrate. A novel two-step bonding method was created to assemble the complete PMMA microchip. A low limit of detection of 1.25 mg ml À1 for Na + and high separation efficiency of 77 000 and 48 000 plates/m for Na + and K + were obtained when operating the detector at a low excitation frequency of 60 kHz.

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“…Much attention has been paid to capillary electrophoresis (CE) microchips, due to their advantages of high performance, design flexibility, reagent economy, high throughput, miniaturization, and automation. [24][25][26][27] These microchip analysis systems hold considerable promise for biomedical and pharmaceutical analysis, clinical diagnostics, environmental monitoring, and forAbstract: We describe the development and application of a novel carbon nanotube/poly(methyl methacrylate) (CNT/PMMA) composite electrode as a sensitive amperometric detector of microchip capillary electrophoresis (CE). The composite electrode was fabricated by the in situ polymerization of a mixture of CNTs and prepolymerized methyl methacrylate in the microchannel of a piece of fused silica capillary under heat.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotube/Poly(methyl methacrylate) (CNT/PMMA) Composite Electrode Fabricated by In Situ Polymerization for Microchip Capillary Electrophoresis

Yao¹,

Wu²,

Wang³

et al. 2007

Chemistry A European J

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We describe the development and application of a novel carbon nanotube/poly(methyl methacrylate) (CNT/PMMA) composite electrode as a sensitive amperometric detector of microchip capillary electrophoresis (CE). The composite electrode was fabricated by the in situ polymerization of a mixture of CNTs and prepolymerized methyl methacrylate in the microchannel of a piece of fused silica capillary under heat. The performance of this unique system was demonstrated by the separation and detection of phenolic pollutants and purines. The new CNT-based CE detector offered significantly lower operating potentials, yielded substantially enhanced signal-to-noise characteristics, and exhibited resistance to surface fouling and, hence, enhanced stability. Long-term stability and reproducibility with relative standard deviations of less than 5 % for the peak current (n=20) were also demonstrated. The simplicity and significant performance exhibited by the CNT/PMMA composite electrode indicate great promise for conventional CE, flowing-injection analysis, and other microfluidic analysis systems.

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Low temperature bonding of poly(methylmethacrylate) electrophoresis microchips by in situ polymerisation

Cited by 34 publications

References 35 publications

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection

Carbon Nanotube/Poly(methyl methacrylate) (CNT/PMMA) Composite Electrode Fabricated by In Situ Polymerization for Microchip Capillary Electrophoresis

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