Low-temperature calorimetry of biological macromolecules

Mrevlishvili, G. M.

doi:10.3367/ufnr.0128.197906c.0273

Cited by 27 publications

(23 citation statements)

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“…Exceptions are a few vibrations (7,24,36,37,38,40, and 41, see Table 1), for which we observe a small amount (~5%) of mixing of the Gua and Cyt vibrations, although they are mainly localized on one of the bases. For all the vibrations of the pair except 24, the vibrations mainly localized on the molecular fragments of the amine and carbonyl groups of Gua and Cyt do mix: for vibrations 7, 40, and 41, these are bending and stretching vibrations; for vibrations 36, 37, 38, these are bending vibrations of the internal angles of the pyrimidine and purine rings adjacent to these fragments.…”

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confidence: 86%

“…A large number of theoretical and experimental papers are available that examine various aspects of the hydrogen bond in DNA, such as the role of the hydrogen bond in formation of the spatial structure and in tautomeric interconversions, the effect of hydrogen bonds on the physicochemical properties and stability of hydrogen-bonded pairs, etc. [1][2][3][4][5][6][7]. One of the most perfect tools for studying the properties of the hydrogen bond is vibrational spectroscopy.…”

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confidence: 99%

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Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

2009

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We have calculated the frequencies of the normal vibrations of the complementary nucleic acid base pairs adenine-thymine, guanine-cytosine, adenine-uracil, corresponding to the Watson-Crick structure, and the adenine-uracil pair, corresponding to the Hoogsteen structure, in condensed states and we interpret the spectra. We determine the contributions of hydrogen bonds to the vibrational modes of the complementary pairs. We have analyzed the nature of the relative displacements of the nucleic acid bases as integral molecular units along the hydrogen bonds. We show the role of hydrogen bonds in tautomeric interconversions of complementary nucleic acid base pairs.Introduction. The hydrogen bond joining nucleic acid base pairs into an integral unit plays a number of roles that allow the DNA to fulfill its main purpose: storing and replicating genetic information. A large number of theoretical and experimental papers are available that examine various aspects of the hydrogen bond in DNA, such as the role of the hydrogen bond in formation of the spatial structure and in tautomeric interconversions, the effect of hydrogen bonds on the physicochemical properties and stability of hydrogen-bonded pairs, etc. [1][2][3][4][5][6][7]. One of the most perfect tools for studying the properties of the hydrogen bond is vibrational spectroscopy. The effect of a hydrogen bond on the vibrational spectrum can be determined by comparing the spectra of the isolated molecule and the molecule in the liquid or solid phase, or the spectra of the isolated molecule and the spectra of isolated dimers or an isolated complex of the molecule, for example with water. In all these cases the position of the bands in the IR spectra and their intensity change, which is the major carrier of information about the effect of a hydrogen bond on the change in structure and properties of the molecule.For nucleic acid base pairs, an analogous study has been carried out in the following directions: the change in the IR and Raman spectra of the complementary pairs has been studied in different phase states and different temperature intervals; the spectra of isolated nucleic acid bases have been compared with the spectra of the complementary pairs; the spectra of the pairs adenine-thymine, guanine-cytosine have been compared with the spectra of the corresponding nucleotides and nucleosides [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Usually study of the experimental spectra is accompanied by nonempirical calculations of the normal vibrations and their interpretations. As shown by analysis of the vibrational spectra, in the range 200-1800 cm -1 major changes are observed in the spectra of complementary pairs compared with the

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Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

2009

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“…The DNA melting temperature T 0 may be defined as the first moment of the differential melting curve (-d8/dT): [7) After integration we have the following expression [8) Here TGC s = f (1-8) dT TAT is numerically equal to the square limited by the melting curve (1 -8(T)) the temperature axis and the T = TGc vertical line. It follows from Equation [8) that T 0 varies if the shape and place of melting curve change.…”

Section: Resultsmentioning

confidence: 99%

“…The calorimetry method is well developed to determine energetic properties ofbiopolymers (7). However it is very significant to have a method to obtain the value of ~H in the conditions where the influence of denaturation of DNA on pH of solution as well as the intermolecular interactions could be neglected.…”

Section: Introductionmentioning

confidence: 99%

Enthalpy of Helix-Coil Transition of DNA: Dependence on Na⁺Concentration and GC-Content

Karapetian¹,

Вардеванян

Frank‐Kamenetskii

1990

Journal of Biomolecular Structure and Dynamics

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The enthalpy of helix-coil transition of DNA (delta H) is determined from the experiments on DNA melting with ligands by means of absolutely general formula, which contains only values directly known from the experiment (M.D. Frank-Kamenetskii, and A.T. Karapetian, Mol. Biol. USSR 6, 621 (1972)) with the combination of the "area" method (P.O. Vardevanian, et al., Biophysica 28, 130 (1983)). The experimentally obtained data show that delta H depends on both concentration of Na+ in solution and GC-content of DNA and is of high accuracy.

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“…As mentioned before, heat capacity depends entirely on the material's composition . Previous works have shown that fibrillar proteins exhibit a larger heat capacity than other protein families . For example, globular proteins such as insulin, albumin, and chymotrypsinogen‐A possess a heat capacity value around 30 cal/100 g °K, whereas it increases more than ten times for type I collagen .…”

Section: Discussionmentioning

confidence: 97%

Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated‐differential scanning calorimetry for research in hepatology

Escobedo

Arjona‐Román

Meléndez‐Pérez

et al. 2012

Hepatology Research

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Low-temperature calorimetry of biological macromolecules

Cited by 27 publications

References 5 publications

Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

Enthalpy of Helix-Coil Transition of DNA: Dependence on Na⁺Concentration and GC-Content

Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated‐differential scanning calorimetry for research in hepatology

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Low-temperature calorimetry of biological macromolecules

Cited by 27 publications

References 5 publications

Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

Calculation and analysis of vibrational spectra of adenine–thymine, guanine–cytosine, and adenine–uracil complementary pairs in the condensed state

Enthalpy of Helix-Coil Transition of DNA: Dependence on Na+Concentration and GC-Content

Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated‐differential scanning calorimetry for research in hepatology

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Enthalpy of Helix-Coil Transition of DNA: Dependence on Na⁺Concentration and GC-Content