Low temperature emergence potential of short season corn hybrids grown under controlled environment and plot conditions

Hope, H. J.; Maamari, R.; Séguin, Sylvie; Hamilton, R. I.; Dwyer, L. M.; White, Ronald P.

doi:10.4141/cjps92-009

Cited by 23 publications

(10 citation statements)

References 16 publications

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“…As a consequence, maize is inherently sensitive to low temperatures [ 2 ], particularly during germination, and thus is seldom cultivated at higher latitudes or in mountainous regions. When maize is cultivated in cold zones, the plants grow more slowly and have a relatively short growing season, which leads to lower seedling vitality and reduced yields [ 3 , 4 ]. It is known that the minimum temperature for maize seed germination is approximately 10 °C.…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches

Zhang

et al. 2020

BMC Plant Biol

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Background Maize ( Zea mays L.) is one of the main agricultural crops with the largest yield and acreage in the world. However, maize germplasm is very sensitive to low temperatures, mainly during germination, and low temperatures significantly affect plant growth and crop yield. Therefore, the identification of genes capable of increasing tolerance to low temperature has become necessary. Results In this study, fourteen phenotypic traits related to seed germination were used to assess the genetic diversity of maize through genome-wide association study (GWAS). A total of 30 single-nucleotide polymorphisms (SNPs) linked to low-temperature tolerance were detected (−log10( P ) > 4), fourteen candidate genes were found to be directly related to the SNPs, further additional 68 genes were identified when the screen was extended to include a linkage disequilibrium (LD) decay distance of r 2 ≥ 0.2 from the SNPs. RNA-sequencing (RNA-seq) analysis was then used to confirm the linkage between the candidate gene and low-temperature tolerance. A total of ten differentially expressed genes (DEGs) (|log 2 fold change (FC)| ≥ 0.585, P < 0.05) were found within the set distance of LD decay ( r 2 ≥ 0.2). Among these genes, the expression of six DEGs was verified using qRT-PCR. Zm00001d039219 and Zm00001d034319 were putatively involved in ‘mitogen activated protein kinase (MAPK) signal transduction’ and ‘fatty acid metabolic process’, respectively, based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Thus, these genes appeared to be related to low-temperature signal transduction and cell membrane fluidity. Conclusion Overall, by integrating the results of our GWAS and DEG analysis of low-temperature tolerance during germination in maize, we were able to identify a total of 30 SNPs and 82 related candidate genes, including 10 DEGs, two of which were involved in the response to tolerance to low temperature. Functional analysis will provide valuable information for understanding the genetic mechanism of low-temperature tolerance during germination in maize.

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Section: Introductionmentioning

confidence: 99%

Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches

Zhang

et al. 2020

BMC Plant Biol

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“…M AIZE is one of the most economically important plants grown in North America and is often subjected to cool conditions after sowing, which can disrupt development (Cutworth and Shaykewich, 1990;Hope et al, 1992). Maize lines that respond well under chilling conditions in temperate regions would aid in optimizing yields.…”

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confidence: 99%

“…Few previous methods of determining the degree of tolerance of maize lines to chilling have used inbred lines (Mock and McNeill, 1979); most studies have involved hybrids (Markowski, 1988;Hope et al, 1992). Inbred lines tend to be more susceptible to cold than hybrids (Janowiak and Markowski, 1987).…”

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confidence: 99%

A Chilling Response Test for Early Growth Phase Maize

Hodges

Hamilton²,

Charest

1995

Agronomy Journal

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Results from a new laboratory screening technique for the determination of chilling responses in maize (Zea mays L.) at the early growth phase were compared with those obtained from the field. This laboratory technique consisted of growing plants hydroponically in Styrofoam rafts floated on Hoagland's solution at a constant low temperature. Seedlings of eight inbred maize lines were pinned into the rafts and placed in controlled environment chambers at 11°C and 98% relative humidity for 28 d until the four‐leaf stage. A control test used a constant 25°C for 7 d, at which point all lines were at the four‐leaf growth stage. Total, shoot, and root dry mass, and the shoot and root water content were the responses measured. Comparisons between the chilling and the chilling/control ratios showed that they were good indicators of chilling tolerance. Field trials of the same eight inbreds were sown in Ottawa (45°24′ N, 75°43′ W) in the early spring of 1992 and 1993 and the shoot dry mass recorded at the four‐leaf stage. The chill/control ratios of the shoot dry mass of seven of the eight lines from the laboratory were correlated with the averaged field shoot dry mass (r = 0.780, P = 0.032). Thus, this laboratory screening procedure was successful in that the lines indicated by shoot dry mass to be the most chilling resistant or the most sensitive in the laboratory responded similarly in the field.

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“…Under these conditions, seeds normally won't germinate and the growth of seedlings will stop [1]. Thus 10℃ has normally been chosen as the growth conditions for the identification and screen of proper maize germplasm [3,4,5]. In recent years, the occurrence of cold weather becomes more uncertain and low temperature weather occurs more frequently, especially during the germination period and early developmental stage of the seedlings.…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate genes for low temperature tolerance during germination of maize using a genome-wide association study and RNA-sequencing

Zhang

et al. 2020

Preprint

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Background: Maize ( Zea mays L.) is the largest agricultural crop in the world based on acreage and yield, however, it is inherently sensitive to low temperatures. The growth and yield of maize can be affected by low temperature during its whole growth period, particularly during germination. Therefore, it is urgent to identify the new gene(s) related to the low temperature tolerance during maize germination. Results: In this study, 14 phenotypic traits related to seed germination were used to explore the genetic architecture of maize through genome-wide association analysis (GWAS). A total of 30 single nucleotide polymorphisms (SNPs) associated with low temperature tolerance were detected (–log10( P ) > 4); 14 candidate genes were detected as being directly associated with these SNPs and 81 candidate genes were identified when the screen was extended to a distance of 30 kb from these SNPs. The candidate genes were predicted by conjoint analysis with RNA-sequencing (RNA-seq) to evaluate whole-genome gene expression levels. A total of nine differentially expressed genes (DEGs) (|log2foldchange|≥0.585， P <0.05) were found within distance of 30 kb, including two DEGs ( GRMZM2G101383 and GRMZM2G402584 ), which were associated with SNPs directly. The differential expression of these candidate genes was verified using qRT-PCR. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms of DEGs GRMZM2G052129 and GRMZM2G038964 were, ‘fatty acid metabolic process’, ‘Mitogen-activated protein kinase (MAPK) signal transduction’, and so on, which are related to the fluidity of the cell membrane and low temperature signal transduction. Conclusion: Therefore, further functional analysis of GRMZM2G052129 and GRMZM2G038964 will provide valuable information for understanding the genetic mechanism of low temperature tolerance during germination in maize.

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Low temperature emergence potential of short season corn hybrids grown under controlled environment and plot conditions

Cited by 23 publications

References 16 publications

Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches

Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches

A Chilling Response Test for Early Growth Phase Maize

Identification of candidate genes for low temperature tolerance during germination of maize using a genome-wide association study and RNA-sequencing

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