Low temperature organic phase biocatalysis using cold-adapted enzymes

Owusu‐Apenten, Richard

doi:10.1007/978-3-642-58607-1_3

Cited by 5 publications

(1 citation statement)

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“…Cold‐adapted enzymes have potential application in mixed aqueous‐organic or non‐aqueous solvents for the purpose of organic synthesis. Their utility derives from their inherent flexibility, which counteracts the stabilizing effects of low water activity in organic solvents ( Owusu Apenten, 1999 ; Sellek and Chaudhuri, 1999 ; Gerday et al ., 2000 ). Cold‐adapted esterases and lipases have been found to exhibit a high level of stereospecificity during fine chemical synthesis ( Aurilia et al ., 2008 ; Joseph et al ., 2008 ; Tutino et al ., 2009 ).…”

Section: Overview Of the Use Of Cold‐adapted Enzymes For Biotechnologmentioning

confidence: 99%

Biotechnological uses of enzymes from psychrophiles

Cavicchioli

Charlton

Ertan

et al. 2011

Microbial Biotechnology

300

169

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SummaryThe bulk of the Earth's biosphere is cold (e.g. 90% of the ocean's waters are ≤ 5°C), sustaining a broad diversity of microbial life. The permanently cold environments vary from the deep ocean to alpine reaches and to polar regions. Commensurate with the extent and diversity of the ecosystems that harbour psychrophilic life, the functional capacity of the microorganisms that inhabitat the cold biosphere are equally diverse. As a result, indigenous psychrophilic microorganisms provide an enormous natural resource of enzymes that function effectively in the cold, and these cold‐adapted enzymes have been targeted for their biotechnological potential. In this review we describe the main properties of enzymes from psychrophiles and describe some of their known biotechnological applications and ways to potentially improve their value for biotechnology. The review also covers the use of metagenomics for enzyme screening, the development of psychrophilic gene expression systems and the use of enzymes for cleaning.

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