Low-tidal-volume prevent ventilation induced inflammation in a mouse model of sepsis

Boehm, Olaf; Rohner, Marc; Ehrentraut, Heidi; Guenther, Ulf; Meyer, Rainer; Knuefermann, Pascal; Baumgarten, Georg; Duerr, Georg Daniel; Velten, Markus

doi:10.1016/j.lfs.2019.117081

Cited by 7 publications

(2 citation statements)

References 35 publications

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“…The mouse model of LPS-induced sepsis is well established, and mirrors gram-negative bacterial infections in a standardized manner. 21,22 LPS is a potent activator of inflammatory responses, leading to cytokine release and leukocyte recruitment. 23 In the peritoneal cavity, the resident leukocyte population is composed of 45%-90% macrophages, 2%-6% dendritic cells, and less than 5% neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Vancomycin and daptomycin modulate the innate immune response in a murine model of LPS-induced sepsis

Muenster

Zschernack

Dierig³

et al. 2021

Int J Immunopathol Pharmacol

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Sepsis is a leading cause of death worldwide, despite the use of multimodal therapies. Common antibiotic regimens are being affected by a rising number of multidrug-resistant pathogens, and new therapeutic approaches are therefore needed. Antibiotics have immunomodulatory properties which appear to be beneficial in the treatment of sepsis. We hypothesized that the last-resort antibiotics vancomycin (VAN) and daptomycin (DMC) modulate cell migration, phagocytosis, and protein cytokine levels in a murine model of lipopolysaccharide (LPS)-induced sepsis. Ten to twelve-week-old C57BL/6 mice ( n = 4–6 animals per group) were stimulated with LPS for 20 h, followed by the administration of VAN or DMC. The outcome parameters were leukocyte accumulation and effector function. Quantification of the immune cells in the peritoneal lavage was performed using flow cytometry analysis. Phagocytosis was measured using pHrodo E. coli BioParticles. The response of the cytokines TNFα, IL-6, and IL-10 was measured in vitro using murine peritoneal macrophages stimulated with LPS and VAN or DMC. VAN decreased both the peritoneal macrophage and the dendritic cell populations following LPS stimulation. DMC reduced the dendritic cell population in the peritoneal cavity in LPS-infected mice. Both antibiotics increased the phagocytic activity in peritoneal macrophages, but this effect was diminished in response to LPS. Phagocytosis of dendritic cells was increased in LPS-infected animals treated with VAN. VAN and DMC differently modulated the levels of pro-and anti-inflammatory cytokines. In a murine model of LPS-induced sepsis, VAN and DMC exhibit immunomodulatory effects on cells involved in innate immunity. The question of whether these antibiotics exhibit synergistic effects in the treatment of septic patients, beyond their bactericidal properties, should be further evaluated in future studies.

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Section: Discussionmentioning

confidence: 99%

Vancomycin and daptomycin modulate the innate immune response in a murine model of LPS-induced sepsis

Muenster

Zschernack

Dierig³

et al. 2021

Int J Immunopathol Pharmacol

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“…A tidal volume or pressure that is too large can cause hyperventilation, barotrauma, and hemodynamic instability [6], [7]. Tidal volume is the maximum volume of the lungs or the amount of air that goes into and out of breath [8]- [11].…”

Section: Introductionmentioning

confidence: 99%

Design of Ventilator with Gas Mixing, Tidal Volume, and Humidifier Parameters

Fajrin¹,

Susanto²,

Kristianto³

et al. 2022

Journal of Robotics and Control

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On the ventilator, there are several important parameters, including gas mixing, which functions to mix oxygen with free air, the tidal volume serves to supply mixed air to the patient, respiratory rate is the frequency of breath given to the patient, and the humidifier functions to regulate the temperature of the air given to the patient. In this research, the author intends to design a ventilator device which uses a working system to open and close the valve to distribute air to the patient. This tool uses several sensors: oxygen sensor KE-25F3, flow sensor yf-s201, pressure sensor MPX 5700, and temperature sensor DS 12B20. The tidal volume (VT) has 3 setting values: 700 ml, 500 ml, and 300 ml. The test is carried out by opening and closing the valve. The respiratory rate has 2 settings of 15 and 20 breaths/minute. In addition, the humidifier has 3 setting modes 32, 35, and 40o C. From the test results, the highest error was obtained in the 300 ml tidal volume test, which was 7.20% and in the respiratory rate test, the highest error value was 0%. The test results with the oxygen concentration parameter obtained the largest error value of 0.1% at 100% oxygen concentration. In testing the temperature and humidity parameters, the largest average error was 2.40% at 40o C setting. So, it can be concluded that the tool is feasible to use because of the level of small error and still within the standard calibration tolerance of 15%.

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