Ligno-cellulosic natural fiber like Banana can be acclimated to manufacture hybrid or union fabric with mostly used jute fiber which can get colossal popularity as a wearable product because of the health and hygiene issues. Banana fibers, extracted from the sheath, can be utilized for manufacturing fiber, can diminish the pressure on the jute fiber. It will be very frugal, and ineluctably it will be a great opportunity to blend it with the other natural fiber. In this study, a Jute-Banana hybrid fabric (JBHF) was developed by using Jute yarn (12 Ne, in warp) and Banana yarn (18 Ne, in weft). In order to evaluate physico-mechanical properties of naturally dyed Jute-Banana hybrid fabric, different tests like Stiffness, Thickness, Tensile strength, Tear strength, Drape and Crease recovery were conducted which provided satisfactory results. Colorfastness to wash, water, rubbing and perspiration of naturally dyed Jute-Banana hybrid fabrics (JBHF) also provided acceptable outcomes. FTIR test also provided the confirmation of this two fibers and Spectrophotometer was used to illustrate the dyeing quality. It was an endeavor to minimize the dependency on a single quality fiber and find an alternative way to prepare the hybrid fabric by blending two different quality natural fibers.