2013
DOI: 10.1002/elan.201200648
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Low‐Voltage Pulsed Electric Field Sterilization on a Microfluidic Chip

Abstract: A polyimide substrate based microfluidic chip with thousands of comb-shaped microelectrodes has been designed, fabricated, and tested for sterilization of bacteria by using pulsed electric field. The performance of bacteria sterilization as functions of the electric field strength, pulse number and width, treatment buffer, bacteria growth status, and bacteria enrichment by positive dielectrophoresis has been experimentally investigated on the microfluidic chip. Experimental results show that only 100 V are suf… Show more

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Cited by 8 publications
(4 citation statements)
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“…A microchip with coplanar electrode arrays of symmetrical comb teeth shown in Figure 1 b was designed and fabricated based on the flexible printed circuit board (FPCB) technique following reference [ 17 ]. The raised comb teeth in the polyimide substrate-based microchip formed a subcell for GUV formation on one hand, and strengthened the electric field on the other hand.…”
Section: Methodsmentioning
confidence: 99%
“…A microchip with coplanar electrode arrays of symmetrical comb teeth shown in Figure 1 b was designed and fabricated based on the flexible printed circuit board (FPCB) technique following reference [ 17 ]. The raised comb teeth in the polyimide substrate-based microchip formed a subcell for GUV formation on one hand, and strengthened the electric field on the other hand.…”
Section: Methodsmentioning
confidence: 99%
“…It takes 3–5 min to align a single cell, parallelization is challenging, and optical forces may not be sufficiently strong to easily move particularly sticky cells . Instead, DEP forces have been used frequently to position cells in microscale EP devices. ,,,,, Unlike optical tweezers, DEP forces are effective for large numbers of cells in parallel. DEP forces are exerted on polarizable particles using a nonuniform electric field to manipulate cells at the microscale and have been widely used for microfluidic cell sorting. , The earliest example of DEP to assist microscale EP used triangular electrode arrays to draw cells toward sharp-edged electrodes in a microfluidic channel for yeast, bacteria, plant, and mammalian cell lysis at low voltages. , Kim et al used DEP forces to guide bacteria into a microwell array with single-cell trapping. The DEP forces were stronger than bacteria motility to restrict cells from swimming out of the microwells.…”
Section: Technological Improvementsmentioning
confidence: 99%
“…The configuration of the microelectrode arrays influenced the bacterial sterilization. Firstly, the bacteria were concentrated in the high electric field region by dielectrophoresis and then the enriched bacteria were killed by the pulsed electric field through microelectrode arrays [ 77 ].…”
Section: Microfluidics Devices Accompanied With Supporting Technolmentioning
confidence: 99%