Low-waste technology for the production of bioethanol from sorghum grain: Comparison of Zymomonas mobilis and Saccharomyces cerevisiae in fermentation with stillage reusing

Szambelan, Katarzyna; Szwengiel, Artur; Nowak, J.; Jeleń, Henryk H.; Frankowski, Jakub

doi:10.1016/j.jclepro.2022.131607

Cited by 7 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VOC emissions are also related to plant species [64,65] and environmental factors [63,[66][67][68][69][70][71][72]. Scientific research confirms the wide possibilities of using sorghum in various industrial fields [43][44][45][46][47][48][70][71][72].…”

Section: Introductionmentioning

confidence: 83%

The Content of Antioxidant Compounds and VOCs in Sorghum Grain Grown in Central and Eastern Europe

Przybylska-Balcerek,

Frankowski,

Sieracka

et al. 2024

Agronomy

Self Cite

View full text Add to dashboard Cite

Sorghum is a plant belonging to the Poaceae family. It is drought-resistant and has low soil requirements. In the face of climate change, it is increasingly cultivated in Europe. Poland is a country with great agricultural potential; it is thus important to develop effective and economic methods of agricultural production, which is confirmed by the introduction of sorghum into cultivation. The aim of this study was to characterize the composition of bioactive compounds (i.e., phenolic acids, flavonoids, carotenoids, and phytosterols) and VOCs in sorghum grain of two varieties, i.e., white ‘Sweet Caroline’ and red ‘Sweet Susana’ grown in the temperate climate (Pętkowo, Poland (52°12′40″ N 17°15′31″ E)). The following tests were carried out: analysis of phenolic acids, flavonoids, carotenoids, phytosterols, antioxidant activity (ABTS), free phenolic acids (FPAs); elemental analysis; and water, fat and starch content analysis. Based on the conducted research, it was concluded that Poland has appropriate conditions for growing sorghum, as the content of bioactive (antioxidant) compounds was at a similar level to those grown in tropical and subtropical climates. Of the nine phenolic acids and seven flavonoids determined, the highest concentrations in both sorghum grain varieties were found for ferulic, p-coumaric and protocatechuic acids. The content of ferulic acid was three times higher in Sweet Caroline grains than in Sweet Susana grains. Differences in the content of these compounds may be the result of genetic differences between the Sweet Susana and Sweet Caroline varieties.

show abstract

Section: Introductionmentioning

confidence: 83%

The Content of Antioxidant Compounds and VOCs in Sorghum Grain Grown in Central and Eastern Europe

Przybylska-Balcerek,

Frankowski,

Sieracka

et al. 2024

Agronomy

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reducing sugars concentration was determined by liquid chromatography‐mass spectrometry (LC‐MS) equipped with an ACQUITY UPLC® BEH Amide 1.7 μm column (Waters Corp., Milford, MA, USA) and the MS detector as described by Szambelan et al 50 …”

Section: Methodsmentioning

confidence: 99%

“…Protein content was estimated as total nitrogen (N × 6.25) by the Kjeldahl method. 49 Reducing sugars concentration was determined by liquid chromatography-mass spectrometry (LC-MS) equipped with an ACQUITY UPLC® BEH Amide 1.7 μm column (Waters Corp., Milford, MA, USA) and the MS detector as described by Szambelan et al 50 The fermented mashes (without solids separation) were distilled on a Super Dee Digital Distillator (Gibertini, Paderno Dugnano, Italy) and ethanol concentration was measured using a DDM 2909 Automatic Density Meter (Rudolph Research Analytical, Hackettstown, NJ, USA) using a mechanical oscillator method. A density meter measures the density of the sample and automatically converts the density to alcohol concentration using the officially recognized AOAC alcohol table (http://rudolfresearch.com/ products/density-meters/ddm-2911/).…”

Section: Methodsmentioning

confidence: 99%

Bioethanol production from sorghum grain with Zymomonas mobilis: increasing the yield and quality of raw distillates

et al. 2023

Self Cite

View full text Add to dashboard Cite

BACKGROUND The present study aimed to demonstrate the superiority of bioethanol yield and its quality from sorghum using the granular starch degrading enzyme Stargen™ 002 over simultaneous saccharification and fermentation, and separate hydrolysis and fermentation using Zymomonas mobilis CCM 3881 and Ethanol Red® yeast. RESULTS Bacteria were found to produce ethanol at higher yield than the yeast in all fermentations. The highest ethanol yield was obtained with Z. mobilis during 48 h of simultaneous saccharification and fermentation (83.85% theoretical yield) and fermentation with Stargen™ 002 (81.27% theoretical yield). Pre‐liquefaction in fermentation with Stargen™ 002 did not improve ethanol yields for both Z. mobilis and Saccharomyces cerevisiae. Chromatographic analysis showed twice less total volatile compounds in distillates obtained after bacterial (3.29–5.54 g L−1) than after yeast (7.84–9.75 g L−1) fermentations. Distillates obtained after bacterial fermentation were characterized by high level of aldehydes (up to 65% of total volatiles) and distillates obtained after yeast fermentation of higher alcohols (up to 95% of total volatiles). The process of fermentation using granular starch hydrolyzing enzyme cocktail Stargen™ 002 resulted in low amounts of all volatile compounds in distillates obtained after bacterial fermentation, but the highest amounts in distillates obtained after yeast fermentation. CONCLUSION The present study emphasizes the great potential of bioethanol production from sorghum with Z. mobilis using granular starch hydrolyzing enzyme Stargen™ 002, which leads to reduced water and energy consumption, especially when energy sources are strongly related to global climate change. © 2023 Society of Chemical Industry.

show abstract

“…Saccharomyces , Schizosaccharomyces , Candida, and Aspergillus other microorganisms are common microorganisms used for fermentation to produce fatty alcohols. In addition, Clostridium thermocellum and Zymomonas mobilis are also reported to be used in the synthesis of fatty alcohols (Hon et al 2017 ; Szambelan et al 2022 ). Carbohydrates (e.g., glucose, and xylose) are usually the main carbon sources for fatty alcohol synthesis by microbial fermentation.…”

Section: Conversion Of Co 2 To Fatty Acid Derivati...mentioning

confidence: 99%

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Liu

Luo

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Environmental problems such as greenhouse effect, the consumption of fossil energy, and the increase of human demand for energy are becoming more and more serious, which force researcher to turn their attention to the reduction of CO2 and the development of renewable energy. Unsafety, easy to lead to secondary environmental pollution, cost inefficiency, and other problems limit the development of conventional CO2 capture technology. In recent years, many microorganisms have attracted much attention to capture CO2 and synthesize valuable products directly. Fatty acid derivatives (e.g., fatty acid esters, fatty alcohols, and aliphatic hydrocarbons), which can be used as a kind of environmentally friendly and renewable biofuels, are sustainable substitutes for fossil energy. In this review, conventional CO2 capture techniques pathways, microbial CO2 concentration mechanisms and fixation pathways were introduced. Then, the metabolic pathway and progress of direct production of fatty acid derivatives from CO2 in microbial cell factories were discussed. The synthetic biology means used to design engineering microorganisms and optimize their metabolic pathways were depicted, with final discussion on the potential of optoelectronic–microbial integrated capture and production systems.

show abstract

Low-waste technology for the production of bioethanol from sorghum grain: Comparison of Zymomonas mobilis and Saccharomyces cerevisiae in fermentation with stillage reusing

Cited by 7 publications

References 35 publications

The Content of Antioxidant Compounds and VOCs in Sorghum Grain Grown in Central and Eastern Europe

The Content of Antioxidant Compounds and VOCs in Sorghum Grain Grown in Central and Eastern Europe

Bioethanol production from sorghum grain with Zymomonas mobilis: increasing the yield and quality of raw distillates

Synthetic biology promotes the capture of CO2 to produce fatty acid derivatives in microbial cell factories

Contact Info

Product

Resources

About