LptD-antigen system on gold nanoparticles: an innovative strategy in the nanovaccine development

Abstract

Nanovaccine development is a growing research field in which the development of new carriers and bioconjugation approaches is a priority. In this sense, this report describes for the first time, the development of a novel conjugate that consists of gold nanoparticles (AuNPs) obtained by a one-step synthesis using an immunogenic peptide of the Lipopolysaccharide-assembly protein LptD from Vibrio parahaemolyticus bacteria as a reducing and capping agent. The resulting LptD@AuNPs compounds were fully characterized and the results showed the high capacity of the peptide to form complexes and reduce gold ions. The reaction yield estimated was higher than 83% and the chemical integrity of the peptide on the NP surface revealed a tyrosine amino acid bonding on the AuNP surface. Furthermore, the LptD@AuNP system showed high colloidal stability in a wide pH range (3-11 pH values), where the hydrodynamic diameter and Zeta potential behavior were strongly influenced by the functional groups of the antigenic peptide. The cytotoxicity assays showed that the obtained system is safe for mouse leukocytes, while immunized mice with LptD@AuNPs produced specific IgG antibodies. These encouraging results revealed the efficacy of some antigenic peptides as reducers and capping agents, in addition, opening the path to determine immunogenicity and immunoprotective efficacy of the LptD@AuNP system against the disease induced by Vibrio parahaemolyticus.

Metal ions and nanometallic materials in antitumor immunity: Function, application, and perspective

“…As we have generally described, the metabolism of several metal ions in the TME, especially concerning antitumor immunity, has been well established. The slightest change in their extra/intracellular concentration results in amplified effects by signaling cascades that determine cell fate and immunity status [ 141 , 142 ]. Moreover, the self-regulation of metal ion metabolism and application of nanometallic materials influence the network of antitumor immunity through various pathways.…”

Use of nanoparticle biosensors to evaluate carnivore health: A new approach

“…This collective oscillation of electrons depends on electron density, effective medium, refraction index, NP shape and size, and separation between NPs. The maximum lambda spectral position (λ max ) indicates the position of the LSPR, and this can be tuned by modulating the size and shape of nanoparticles and changing the physicochemical properties of the surface and the medium where NPs are found (Aguilera-Juárez et al 2022). The LSPR is composed of the extinction coefficient, which is the sum of the absorption coefficient and the scattering coefficient (C ext = C abs + C scat ), i. e., of the total light photons interacting with the NP, one part is absorbed and the other is scattered mostly without apparent energy loss (elastic dispersion).…”