<b>DIFFERENCES IN PROLIFERATION PROPERTIES AMONG SUBLINES OF RAT 3Y1 FIBROBLASTS TRANSFORMED BY VARIOUS AGENTS <i>IN </i></b><b><i>VITRO </i></b>

Abstract

Alterations in cellular characteristics associated with transformation were compared among various agents, including simian virus 40, mouse polyoma virus, adenovirus type 12, the ElA region of adenovirus type 12, Rous sarcoma virus, v-Hras oncogene, and N-methyl-N'-nitro-N-nitrosoguanidine. To avoid the difficulty in interpretation due to differences in the original cellular characteristics, all transformed lines (three to four independent lines for each agent) were derived from the same parental line, i.e. rat clonal diploid fibroblasts 3Y1. Doubling time, saturation density, serum requirement, efficiency of colony formation, Ca dependency, anchorage dependency, cell volume, density-dependent inhibition of proliferation, and alteration of morphology were examined. In the lines transformed either by DNA virus, RNA virus, or transforming oncogene, the characteristics were similar among the lines induced by the same transforming agent but distinguishable between the lines induced by different agents. In such cases, a transformed line displayed phenotypes specific to each transforming agent. In contrast, nitrosoguanidine-transformed lines showed different properties. Only one characteristic was shared by all the transformed lines: the ability to attain a higher saturation density than that of the untransformed parent. The fact that (cellular) densitydependent inhibition of DNA synthesis became weak may be responsible for this characteristic.

Mustard Oil in “Shibori Daikon” a Variety of Japanese Radish, Selectively Inhibits the Proliferation of H-ras-Transformed 3Y1 Cells

“…[4][5][6][7] The HR-3Y1-2 cell line established by Kimura et al was derived from 3Y1 rat fibroblasts transformed with the v-H-ras oncogene. 8) Anchorageindependent proliferation and changed cellular morphology are known features of 3Y1 cells transformed with v-H-ras, 9) and our preliminary study has shown that HR-3Y1-2 cells were capable of proliferating faster than the parent 3Y1cell line. Therefore, these cell lines are useful for evaluating the effects of various treatments on the malignant characteristics derived from Ras activation.…”

Characterization of the kinase activity essential for tyrosine phosphorylation of p130Cas in fibroblasts

“…SR-3Y1 is a 3Y1 cell line transformed by v-Src of Rous sarcoma virus (Zaitsu et al, 1988). Abl-negative ®broblasts are kindly given from Dr BJ Mayer.…”

Reversion of Temperature-Sensitive Mutation by Inhibition of Proteasome-Mediated Degradation of Mutated D123 Protein.

“…ts2 and ts3 are SV3Y1tsD123 derivatives overexpressing the mutated D123 protein of 3Y1tsD123, obtained by transfecting SV-3Y1tsD123 with expression plasmid carrying the mutated D123 cDNA and the hygromycin B marker (Okuda et al, 1999). SV-3Y1-C66 (referred to as SV-3Y1) is an SV40-transformed derivative of 3Y1 (Zaitsu et al, 1988).…”